Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,081,698	T→C	T284A (ACG→GCG)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,698	0	T	C	100.0%	85.9 / NA	25	T284A (ACG→GCG)	dacD	D‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/16);  total (9/16)

GCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAG  >  NC_000913/2081562‑2081821                                                                                                                                         |                                                                                                                            gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgcagt                                                                                                                          >  2:555272/1‑140 (MQ=255) gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGGTCCGACCATTTTCCCAGGGTGGAAAATTTGCACCgcagt                                                                                                                          >  2:198059/1‑140 (MQ=255)            tgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTttgtt                                                                                                               <  1:602595/140‑1 (MQ=255)                      gCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACg                                                                                                     <  2:141308/140‑1 (MQ=255)                           cGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTa                                                                                                <  1:362869/140‑1 (MQ=255)                           cGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTa                                                                                                <  1:622168/140‑1 (MQ=255)                                 cACCATCCAGAACTCCTGTTCCCTTCCCAGGTCGATACTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTc                                                                                          <  1:294943/140‑1 (MQ=255)                                      tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATGTGCACCGCAGTAGAGTTTTGTTGCCGCCAACGCAGTAATTTTCTTGcc                                                                                     >  2:294943/1‑140 (MQ=255)                                               ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                                                            <  1:130755/140‑1 (MQ=255)                                                 tGTTCCGTTCCCAGGTCGATATCTTCTTTATAGACGTACCAGATGCGTGCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACg                                                                          <  2:312659/140‑1 (MQ=255)                                                        ttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTACCTCTTCCTCACGACCtttt                                                                   >  1:433123/1‑140 (MQ=255)                                                          cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc                                                                 >  2:372013/1‑140 (MQ=255)                                                          cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAAAGCAGTAAATTTCTTGCCTCTTCCTCACGACCTTTTGc                                                                 >  1:312659/1‑140 (MQ=255)                                                           ccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                                                                <  2:409676/140‑1 (MQ=255)                                                            cAGGTCGATATTTTCCTTATCGCCATACCGGATGCGTGCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCAc                                                               <  1:238450/140‑1 (MQ=255)                                                                  gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg                                                         <  2:543246/140‑1 (MQ=255)                                                                  gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACACAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg                                                         <  2:525787/140‑1 (MQ=255)                                                                    tatTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                       <  2:397452/140‑1 (MQ=255)                                                                    agacagcTTTATCGCCATACCACATGCGTTCGGTTTCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                       <  2:243096/134‑1 (MQ=255)                                                                                     tACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCAGGACCTTTTGCACTGTCAGCCCGCATTACCAcctgtct                                      >  1:243096/1‑134 (MQ=255)                                                                                               gttccgttccGACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGc                            <  2:511960/140‑1 (MQ=255)                                                                                                ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCt                           >  2:615458/1‑140 (MQ=255)                                                                                                ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCt                           >  1:181802/1‑140 (MQ=255)                                                                                                 tccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTg                          >  2:489671/1‑140 (MQ=255)                                                                                                  ccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGc                         <  2:181802/140‑1 (MQ=255)                                                                                                                     aCGGTGCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCaa      <  2:433123/140‑1 (MQ=255)                                                                                                                         ggCAAAATTTGCACCGCAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgag  >  2:57246/2‑140 (MQ=255)                                                                                                                                         |                                                                                                                            GCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAG  >  NC_000913/2081562‑2081821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑