Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,125,570	T→C	K59K (AAA→AAG)	wcaI ←	putative colanic biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,125,570	0	T	C	100.0%	121.0 / NA	34	K59K (AAA→AAG)	wcaI	putative colanic biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/23);  total (11/23)

ACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGTTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATG  >  NC_000913/2125441‑2125699                                                                                                                                  |                                                                                                                                  aCGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCTTGGCGGCCCCCTCTTCTCGCTTGTAGCGcc                                                                                                                         <  2:449149/140‑1 (MQ=255) aCGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGcc                                                                                                                         <  2:29766/140‑1 (MQ=255)    ttGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAgcg                                                                                                                           <  2:509100/135‑1 (MQ=255)    ttGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAgcg                                                                                                                           >  1:509100/1‑135 (MQ=255)                   aaagaaaCTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGc                                                                                                       <  2:158273/140‑1 (MQ=255)                              tgACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGCGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCAt                                                                                            <  1:189796/140‑1 (MQ=255)                               gACGGCAAAACTGCCTAGATGCGACAGGCATTTCAGGATGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCAtt                                                                                           <  1:223730/140‑1 (MQ=255)                                 cGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGc                                                                                         <  1:628445/140‑1 (MQ=255)                                       aaCTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGgtag                                                                                   <  2:46854/140‑1 (MQ=255)                                            cccAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCAtt                                                                                           <  2:625570/127‑1 (MQ=255)                                            cccAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCAtt                                                                                           >  1:625570/1‑127 (MQ=255)                                            cccAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAgg                                                                              <  2:420309/140‑1 (MQ=255)                                                gATGCAACAGGCGTTTCTGGGTCCTCGGCTGCTTTGGCACATACAGCGCGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGgcggt                                                                          <  2:201557/140‑1 (MQ=255)                                                 aTGCAACATGCGTTTCAGGGTGATCGGCTGTTTGGACACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCGCCTGCCATTGCGGGTAGTAAGgcggtg                                                                         <  2:356742/140‑1 (MQ=255)                                                 aTGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGgcggtg                                                                         <  2:459749/140‑1 (MQ=255)                                                           cGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAgg                                                                              >  2:361881/1‑125 (MQ=255)                                                           cGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAgg                                                                              <  1:361881/125‑1 (MQ=255)                                                           cGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGAc                                                               >  2:435344/1‑140 (MQ=255)                                                           cGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGAc                                                               >  1:68099/1‑140 (MQ=255)                                                            gTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGAcc                                                              >  1:509925/1‑140 (MQ=255)                                                            gTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGAcc                                                              <  2:91874/140‑1 (MQ=255)                                                              ttCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGTCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCg                                                            <  2:327091/140‑1 (MQ=255)                                                              ttCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCg                                                            <  1:166007/140‑1 (MQ=255)                                                              ttCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCg                                                            <  1:435344/140‑1 (MQ=255)                                                                     tGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTa                                                                    >  2:121373/1‑125 (MQ=255)                                                                     tGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTa                                                                    <  1:121373/125‑1 (MQ=255)                                                                                                 gCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGGCCTTGTGCCCCCAGCCATTccacca                         >  1:294727/1‑140 (MQ=255)                                                                                                   aGCGCCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCAtc                       >  1:564651/1‑140 (MQ=255)                                                                                                    gcgcCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCAATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCAtct                      <  1:395442/140‑1 (MQ=255)                                                                                                    gcgcCACACCGTGGCCGCCCCCGCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCAtct                      <  1:359900/140‑1 (MQ=255)                                                                                                     cgcCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCgccagcc                                  >  1:473833/1‑127 (MQ=255)                                                                                                     cgcCACACCGTGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCgccagcc                                  <  2:473833/127‑1 (MQ=255)                                                                                                               tGGCGGCCCCCTCTTCTCGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACGTTGTGCCGCCAGCCGTTCCACGATCTCGCCGGTGTAt           >  1:336982/1‑140 (MQ=255)                                                                                                                               tcGCTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATg  <  2:433894/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                                  ACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTTGGCACATACAGCGGGCAGCGCCACACCGTGGCGGCCCCCTCTTCTCGTTTGTAGCGCCAGGCGGAATAGTTCTCGCCCACCTGCCATTGCGGGTAGTAAGGCGGTGCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATG  >  NC_000913/2125441‑2125699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑