| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,104 | A→G | V151A (GTG→GCG) | wcaB ← | putative colanic acid biosynthesis acyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,104 | 0 | A | G | 93.5% | 89.5 / ‑1.8 | 32 | V151A (GTG→GCG) | wcaB | putative colanic acid biosynthesis acyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (11/18); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCAATGTGTGGACATGCC > NC_000913/2131973‑2132238 | gACGCCAGCCCCTGTTTCAGCGCACGCTGGTCGGGTTCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGcgcg < 2:344910/140‑1 (MQ=255) cGCCAGCCCCTGTTGCATCGCATGCTGGTGGTGATGTAATGCCACACCCGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGtt < 2:331686/140‑1 (MQ=255) cAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTc > 2:416302/1‑140 (MQ=255) cAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTc < 1:636405/140‑1 (MQ=255) cAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCCTCACTCGTGCTGTTGCTCCCATCGCCAGCGCGTTGTc > 1:344910/1‑140 (MQ=255) tGTTGCAGCGCACGCTGGTGGATATCTAACGCCACCCCTTCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACa < 2:278427/140‑1 (MQ=255) gCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCATTTTCTCTCACGGCCAGCGCGGTGTCCGGGACAGAGt > 1:233109/1‑140 (MQ=255) cAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTc < 1:669806/140‑1 (MQ=255) cAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTc < 1:619391/140‑1 (MQ=255) gagaTCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGcc < 1:416302/140‑1 (MQ=255) tCTAACGCCACACCTGCCGCCCGGCCTTCCGTCAGGCGCGCATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCcgcg < 2:233109/140‑1 (MQ=255) cTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCTTTGTCCGCGACTGAATCGAGCGCCACGCTGCCCgcgc > 1:210597/1‑140 (MQ=255) aaCGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGccc > 1:37287/1‑140 (MQ=255) gCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACg > 1:394229/1‑140 (MQ=255) cacCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGTACCACGCTGACCGCGCCCACGGTGa > 1:355193/1‑140 (MQ=255) cacCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGa < 1:659729/140‑1 (MQ=255) cacCTGCTGCCCCGCCGTCCGCCAGTCGCACGTTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGa < 2:328168/140‑1 (MQ=255) ccTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACg < 2:677380/140‑1 (MQ=255) tgctgcCCCGCCTTCGGCCAGTTGCCCATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACgtt < 1:320951/140‑1 (MQ=255) ctgcCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAATCGAGCAACACCCTCCCCGCGCCCACGGTGACgttgt > 1:300088/1‑140 (MQ=255) gCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAg > 2:68917/1‑140 (MQ=255) tCCGCCAGTCGCACATTCAATTCCAAAATATTCATCTAACTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTg < 2:210597/140‑1 (MQ=255) gTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCAc > 1:411672/1‑140 (MQ=255) tCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCAcc < 2:411672/140‑1 (MQ=255) tCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCACGCCCCCACGGTGACGATGTTACCAAGCGTGATATCAcc > 2:246320/1‑140 (MQ=255) aaaTTGCAAAATATTCAGTTAATTACCTTCAGCCGTGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGaataat < 2:295586/140‑1 (MQ=255) aaaaTATTCATTTAACTACCTTTACCCGCTCTTTTTCCCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTg < 1:215503/140‑1 (MQ=255) atatTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCa < 2:394229/140‑1 (MQ=255) aTTACCTTCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGAc > 1:60127/1‑140 (MQ=255) tCACTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACAAAGAATAATGAAGTCGGCACCTAGTTCGACGCCGTTg > 1:249433/1‑140 (MQ=255) aCTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGcc < 2:37287/140‑1 (MQ=255) cTCGCGCTTTTTCTCCCACCGCCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCa < 1:146915/140‑1 (MQ=255) ctcCCACCTCCAGCGCTCTGTCCGGGACAGACTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCAATGTGTGGACAt < 2:355193/140‑1 (MQ=255) ccaccgccaGCGCGTTGTCCGGCACAGAGTCGAGCGCCAGGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCAATGTGTGGACATGcc < 2:249433/140‑1 (MQ=255) | GACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCAATGTGTGGACATGCC > NC_000913/2131973‑2132238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |