Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,193,357	A→G	V271A (GTG→GCG)	mrp ←	P‑loop NTPase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,193,357	0	A	G	100.0%	110.3 / NA	31	V271A (GTG→GCG)	mrp	P‑loop NTPase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

CGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCA  >  NC_000913/2193227‑2193485                                                                                                                                   |                                                                                                                                 cGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCt                                                                                                                         >  1:516156/1‑140 (MQ=255)             cagcagCTGTGTATGGTATTTTTCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGa                                                                                                             <  2:471970/140‑1 (MQ=255)              agcagcTGTGTATGGTGATTTTTAGCCAGTTTCTCAGCATCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGaa                                                                                                            <  2:325435/140‑1 (MQ=255)                      tgtATGGTATTTTTCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACaa                                                                                                    <  1:671440/140‑1 (MQ=255)                        tATGGTATTTTTCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATg                                                                                                  >  1:325435/1‑140 (MQ=255)                                ttttCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCTGAACCTCCACTCTCTCGAACACCACAATGCCtttttt                                                                                          >  1:254894/1‑140 (MQ=255)                                ttttCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCtttttt                                                                                          >  1:704844/1‑140 (MQ=255)                                  ttCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCg                                                                                        >  2:491462/1‑140 (MQ=255)                                           tttCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCTGAACCTCGACTTACTCGAACATCACAATGCTTTTTTTCGCATCGATCa                                                                               >  2:216962/1‑140 (MQ=255)                                                  gCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGAt                                                                        <  1:491462/140‑1 (MQ=255)                                                     ccaccGGTACCGAAAATCGGTTCATGGTGACCACACTTAATGCAAATACGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTc                                                                     <  2:198740/140‑1 (MQ=255)                                                        ccGGTACCGAAAATCTGTTCATAGTGACCCCAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTg                                                                  <  1:270408/140‑1 (MQ=255)                                                             aCCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGCACATCACATTGCGTTTATTCGCATCGATCAACGCGATGTCCTGCgggg                                                             >  2:219388/1‑140 (MQ=255)                                                               cGAAAATCGGTTCATGGTGACCCCAGTTACTCCAAATATCCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGggtg                                                           <  2:254894/140‑1 (MQ=255)                                                               cGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGggtg                                                           <  1:649706/140‑1 (MQ=255)                                                               cGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGggtg                                                           >  2:649706/1‑140 (MQ=255)                                                                    aTCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGAc                                                      <  2:445923/140‑1 (MQ=255)                                                                              ggTGACCACAGTTACCGCAAATCTGCACACTCATGTTTTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCa                                            <  1:219388/140‑1 (MQ=255)                                                                                  aCCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCgg                                        >  1:580720/1‑140 (MQ=255)                                                                                  aCCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCgg                                        >  1:444323/1‑140 (MQ=255)                                                                                  aCCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCg                                         >  1:472968/1‑139 (MQ=255)                                                                                  aCCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCg                                         <  2:472968/139‑1 (MQ=255)                                                                                               cAAATATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGtt                           <  2:516156/140‑1 (MQ=255)                                                                                                 aaTATGCACACTCATGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGAGCACGACTGCACCGCTTACTGGAATGtttt                         >  1:300263/1‑140 (MQ=255)                                                                                                               tGTTCTCGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAAt           <  2:663028/140‑1 (MQ=255)                                                                                                                    tcGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGAGCACGACTGCACCGGTTCCTGGAATGATTTGCGCCAGCGTCAATTGAAt      >  2:266545/1‑140 (MQ=255)                                                                                                                    tcGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGt               <  2:391401/131‑1 (MQ=255)                                                                                                                    tcGACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGt               >  1:391401/1‑131 (MQ=255)                                                                                                                      gACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCGCGACTGCAACGGCTACAGGAATGTTTTGCGACAGCGTCAATTGAATGt    >  2:250616/1‑140 (MQ=255)                                                                                                                      gACAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGt    >  1:502211/1‑140 (MQ=255)                                                                                                                        cAATACCGAGCGCCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCa  >  2:608750/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                 CGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTGCACCACCGGTACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCA  >  NC_000913/2193227‑2193485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑