Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,232,893	T→C	F6L (TTC→CTC)	sanA →	DUF218 domain‑containing protein SanA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,232,893	0	T	C	100.0%	87.3 / NA	25	F6L (TTC→CTC)	sanA	DUF218 domain‑containing protein SanA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

TCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGC  >  NC_000913/2232760‑2233028                                                                                                                                      |                                                                                                                                        tCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCa                                                                                                                                   >  2:594089/1‑140 (MQ=255)  ccGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAg                                                                                                                                  <  1:486263/140‑1 (MQ=255)   cGGCTTGCCAGCCCTCTCCTGGGGTCGAATTTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGc                                                                                                                                 <  1:193497/140‑1 (MQ=255)     gCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCt                                                                                                                               >  2:466084/1‑140 (MQ=255)                        gtgtCGAAATTCCCGGCAAACATTTTGCCGTCTCTTGCGCAAAACCAGTGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTtg                                                                                                            <  2:203761/140‑1 (MQ=255)                        gtgtCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTtg                                                                                                            >  2:468492/1‑140 (MQ=255)                        gtgtCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTtg                                                                                                            <  2:702746/140‑1 (MQ=255)                         tgtCGAAATTCCCGGTAAACAGTTTGCCGCTTCTTGCCCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTtgc                                                                                                           <  1:312109/140‑1 (MQ=255)                          gtCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTtgct                                                                                                          <  1:685716/140‑1 (MQ=255)                           tCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTtgctg                                                                                                         >  1:135986/1‑140 (MQ=255)                                   cccGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGAc                                                                                                 >  1:476535/1‑140 (MQ=255)                                                                           gTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACcgcg                                                         <  1:478076/140‑1 (MQ=255)                                                                            gaaaGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCGGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCgcgc                                                        >  2:685788/2‑140 (MQ=255)                                                                              aaGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTCCTCCGCCACGATCGCTGGATGACCTGGAAAACCGCGCCt                                                      >  2:337585/1‑140 (MQ=255)                                                                                      tGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTa                                              <  1:468492/140‑1 (MQ=255)                                                                                        aTGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCTATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTacg                                            >  2:279418/1‑140 (MQ=255)                                                                                        aTGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTacg                                            >  2:51258/1‑140 (MQ=255)                                                                                        aTGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTacg                                            >  2:483879/1‑140 (MQ=255)                                                                                                            gAGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTcccc                        <  1:51258/140‑1 (MQ=255)                                                                                                             aGTCTCCTGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCt                       <  1:483879/140‑1 (MQ=255)                                                                                                                    tGCATGTTAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCa                <  2:135986/140‑1 (MQ=255)                                                                                                                       aTGTTAAAGCGCGTGATCCTCAGCCAGTTGGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGt             <  1:337585/140‑1 (MQ=255)                                                                                                                           tAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGt             >  2:36775/1‑136 (MQ=255)                                                                                                                           tAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGt             <  1:36775/136‑1 (MQ=255)                                                                                                                           tAAAGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGgt         >  2:441798/1‑140 (MQ=255)                                                                                                                              aGCGCGTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGtgtg      <  1:441798/140‑1 (MQ=255)                                                                                                                                  cgTGCTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGc  >  2:76610/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGATCGCTGGATGAGCTGGAAAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGC  >  NC_000913/2232760‑2233028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑