Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,381,001	A→G	R9R (CGT→CGC)	elaB ←	tail anchored inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,381,001	0	A	G	100.0%	123.5 / NA	37	R9R (CGT→CGC)	elaB	tail anchored inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/24);  total (13/24)

TTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATACGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTT  >  NC_000913/2380872‑2381131                                                                                                                                  |                                                                                                                                   ttttttCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCAc                                                                                                                          >  1:551540/1‑140 (MQ=255)     ttCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCATCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCaaa                                                                                                                      <  2:367716/140‑1 (MQ=255)              tcCAGCGCTTTTTCTGCCCGCGTTTTCAGCTCAACATATCTCTGATCGTCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGa                                                                                                             <  2:200031/140‑1 (MQ=255)                    gcTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAGCTGATTAGACATtct                                                                                                       >  1:353395/1‑140 (MQ=255)                    gcTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATtct                                                                                                       >  1:397784/1‑140 (MQ=255)                    gcTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATtct                                                                                                       <  2:397742/140‑1 (MQ=255)                      tttttCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCg                                                                                                     <  1:138549/140‑1 (MQ=255)                       ttttCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGt                                                                                                    >  2:666831/1‑140 (MQ=255)                              cACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCAt                                                                                             <  2:420273/140‑1 (MQ=255)                               aCGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCAtt                                                                                            <  2:570203/140‑1 (MQ=255)                                   gcTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCg                                                                                        <  2:109358/140‑1 (MQ=255)                                                cATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCac                                                                           <  2:718875/140‑1 (MQ=255)                                                cATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCac                                                                           <  2:551540/140‑1 (MQ=255)                                                      tCTGATCGGCGGGATCGCCAGAGGAGCGGAGGCCCCCTTCCAGTGTTTCACTAAGCAGCGGCAGGTCGTCATCGATGCGTGTATCACCCAACTGATTATAAATTATCGTTTTCCATTAGCGTAAAACACGTCactaactt                                                                     >  2:304519/1‑139 (MQ=255)                                                      tCTGATCGGCGGGATCGCCAGAAGAGCGGGGCACCTCATCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCactaacta                                                                     <  1:304519/140‑1 (MQ=255)                                                        tGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACtata                                                                   >  2:565017/1‑140 (MQ=255)                                                          aTCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGa                                                                 >  2:49390/1‑140 (MQ=255)                                                          aTCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGa                                                                 >  1:106724/1‑140 (MQ=255)                                                            cggcggGACCGCCAGAGGAGCGCAGCACCTTCTTCTGTGGTTCACTAATCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACa                                                               <  2:353395/140‑1 (MQ=255)                                                               cggGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCact                                                                          >  2:104851/1‑126 (MQ=255)                                                               cggGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCact                                                                          <  1:104851/126‑1 (MQ=255)                                                                         agagGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGAtt                                                  <  2:530567/140‑1 (MQ=255)                                                                            ggagcggagcACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACACTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACg                                               >  2:559481/1‑140 (MQ=255)                                                                                cggagcACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTg                                           >  1:594965/1‑140 (MQ=255)                                                                                   agcACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAAt                                        <  2:164031/140‑1 (MQ=255)                                                                                              cAGTGTTTCACTAAGCAGCGTCCGGCCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTg                             <  1:237486/140‑1 (MQ=255)                                                                                              cAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTg                             <  2:540835/140‑1 (MQ=255)                                                                                               aGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGc                            <  2:93735/140‑1 (MQ=255)                                                                                                    ttCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATcc                       <  1:49390/140‑1 (MQ=255)                                                                                                     tCACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATccc                      >  1:631095/1‑140 (MQ=255)                                                                                                      cACTAAGCAGCGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCa                     <  2:594965/140‑1 (MQ=255)                                                                                                           agcagcGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAAtgtg                >  1:693789/1‑140 (MQ=255)                                                                                                             cagcGTCAGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACACTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAAtgtgtg              <  1:559481/140‑1 (MQ=255)                                                                                                                    aGGTCGTCATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATAcc       <  2:397784/140‑1 (MQ=255)                                                                                                                    aGGTCGTCATCGATGCGAGTATCACCAAACTGATTCGACTTTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATAcc       <  2:212096/140‑1 (MQ=255)                                                                                                                      gtcgtcATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCAt     <  2:106724/140‑1 (MQ=255)                                                                                                                           cATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCAtctt  >  2:457242/1‑138 (MQ=255)                                                                                                                           cATCGATGCGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCAtctt  <  1:457242/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   TTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGCAGCGTCAGGTCGTCATCGATACGTGTATCACCAAACTGATTAGACATTCTCGTTCTCCATTTGCGTAAAACCTGTCACTAACTATAGACAAGGGTTGTTGATTACGCCTGAATTACCTCGCGTGCCATCCCAATGTGTGGGATACCATCTT  >  NC_000913/2380872‑2381131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑