Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,442,697	T→C	Y312H (TAT→CAT)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,442,697	0	T	C	100.0%	103.8 / NA	30	Y312H (TAT→CAT)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

AGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGA  >  NC_000913/2442561‑2442833                                                                                                                                         |                                                                                                                                         aGCGGCGATTATCGGCGGTTGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATc                                                                                                                                       <  1:71699/140‑1 (MQ=255)       gATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGttat                                                                                                                                 <  1:508781/140‑1 (MQ=255)       gATTATCGGCGGTGGTATTGCCAGCGCGTAGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGttat                                                                                                                                 <  1:322291/140‑1 (MQ=255)                        ttGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAg                                                                                                                <  2:429478/140‑1 (MQ=255)                               cgcgTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTaa                                                                                                         >  2:559703/1‑140 (MQ=255)                                      ttgtCGCTGGCGCTATTACGGCGCGGCTGCCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCtt                                                                                                  <  1:245147/140‑1 (MQ=255)                                       tgtCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCttt                                                                                                 <  1:422669/140‑1 (MQ=255)                                             tGGCGCTAGTACGGCGTAGCTGCCAGGTAAATCCTCATTGCGCGGATGGGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTctct                                                                                           <  2:338607/140‑1 (MQ=255)                                                             cgGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTg                                                                           >  2:25595/1‑140 (MQ=255)                                                               gCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCt                                                                         >  2:658524/1‑140 (MQ=255)                                                               gCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCt                                                                         >  2:714730/1‑140 (MQ=255)                                                                        tAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGACGCGCTAAACCGCTTTTTCTCTAATGCGTTTCCTTTTGCTCGTCGGttt                                                                >  2:318931/1‑140 (MQ=255)                                                                                  ttGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAAt                                                      <  1:412975/140‑1 (MQ=255)                                                                                    gcgcGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTa                                                    <  1:658524/140‑1 (MQ=255)                                                                                     cgcgGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCGTTTTCTCTGATGGGTTGACTTTTGCACGTCGGTTTTACGACCAATTAc                                                   >  2:194494/1‑140 (MQ=255)                                                                                          aTGAGGCCTCCGCACTGGGTGCTTCCGGCACTCTCGAGGTGGCGCTGCGTCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGtt                                              <  2:346913/140‑1 (MQ=255)                                                                                                    cGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATc                                    >  2:32780/1‑140 (MQ=255)                                                                                                    cGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATc                                    >  2:391065/1‑140 (MQ=255)                                                                                                         tGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTg                                       >  2:498489/1‑132 (MQ=255)                                                                                                         tGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTg                                       <  1:498489/132‑1 (MQ=255)                                                                                                           ggTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTg                             >  1:524494/1‑140 (MQ=255)                                                                                                              gCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTg                          >  2:397014/1‑140 (MQ=255)                                                                                                                      cAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCa                  <  1:483096/140‑1 (MQ=255)                                                                                                                              aGGGGGCACTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCa                  <  2:27036/132‑1 (MQ=255)                                                                                                                              aGGGGGCACTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCa                  >  1:27036/1‑132 (MQ=255)                                                                                                                                ggggCGCTGCATCCGTTATTAAGCAGACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAgg        <  1:322570/140‑1 (MQ=255)                                                                                                                                  ggCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCt      <  2:524494/140‑1 (MQ=255)                                                                                                                                    cgcTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTAGGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTgg    >  2:446145/1‑140 (MQ=255)                                                                                                                                    cgcTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTGCCCGTTAATTTTTATCTTGACTGGTGCGGCGTCACGCAGTTAGGCTgg    >  2:301536/1‑140 (MQ=255)                                                                                                                                      cTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGa  <  1:32780/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGA  >  NC_000913/2442561‑2442833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑