Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,579	T→C	T299A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,579	0	T	C	100.0%	123.7 / NA	36	T299A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/15);  total (21/15)

GTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446445‑2446713                                                                                                                                       |                                                                                                                                       gTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCTCCAAAGCGGTTCATTGTACGATCCGGCACGGCAATGc                                                                                                                                   >  1:251815/1‑140 (MQ=255)    aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTgg                                                                                                                                >  1:673324/1‑140 (MQ=255)       cATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAgg                                                                                                                             <  2:654253/140‑1 (MQ=255)           aaaaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTAGCCAAAGCGGTTTACGGTACGACCCGGCACCACAATGCTGGAGGTCgg                                                                                                                         >  2:359229/1‑140 (MQ=255)                 aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTAGGTTTCAg                                                                                                                   <  2:673324/140‑1 (MQ=255)                   cGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgcg                                                                                                                 >  2:121829/1‑140 (MQ=255)                   cGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgcg                                                                                                                 >  2:239016/1‑140 (MQ=255)                    gCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgcgc                                                                                                                >  2:584781/1‑140 (MQ=255)                    gCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgcgc                                                                                                                >  2:40060/1‑140 (MQ=255)                       aGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCat                                                                                                             <  2:704591/140‑1 (MQ=255)                        gCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCata                                                                                                            <  1:74901/140‑1 (MQ=255)                         cATCGCTTCTGCGATCGGCACTTCGCGGATCCCGACACACGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCatat                                                                                                           <  2:271444/140‑1 (MQ=255)                            cGCTTCTGCGATCGGCACTGCGCGGGTCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATggg                                                                                                        <  1:239016/140‑1 (MQ=255)                                         ggCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctg                                                                                           >  2:112331/1‑140 (MQ=255)                                           cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         <  2:739327/140‑1 (MQ=255)                                                cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  1:590006/1‑140 (MQ=255)                                                cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  2:478319/1‑140 (MQ=255)                                                    gaccccGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTCCGCCAAAGCGGTTAATGGTACGCCCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATAGGGGCAATGATTTGCTGCCCGCTGCTGa                                                                                <  2:666572/137‑1 (MQ=255)                                                    gATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGa                                                                                <  1:121829/140‑1 (MQ=255)                                                        ccGACAAAGGATTCGAGAGGTCCTTTGGTGATCATTTCAACTTCTCCGCCAAGGCGGTAATTGGTACGCCCCGGCCCGTCAATGTTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGTTGCTGATAcc                                                                            <  2:507313/140‑1 (MQ=255)                                                                 ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:734633/1‑140 (MQ=255)                                                                 ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:461188/1‑140 (MQ=255)                                                                         aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGTTGCTGATACCGCCGAGAATGccgcccg                                                           <  1:112331/140‑1 (MQ=255)                                                                           ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgcc                                                                <  2:36481/133‑1 (MQ=255)                                                                           ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgcc                                                                <  2:451704/133‑1 (MQ=255)                                                                           ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgcc                                                                >  1:451704/1‑133 (MQ=255)                                                                           ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgcc                                                                >  1:36481/1‑133 (MQ=255)                                                                                ttGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGtt                                                    <  2:377506/140‑1 (MQ=255)                                                                                   gTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGct                                                 <  1:478319/140‑1 (MQ=255)                                                                                     gATCATCTCACCTTCGTCGCCAAAGGGGTCAATGGTAGGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATAAGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGctct                                               <  1:359229/140‑1 (MQ=255)                                                                                         atcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaa                                           >  1:418856/1‑140 (MQ=255)                                                                                                   cttcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGATAATGCCGACCGCATGGTTGCTGTGGAAACCGTCTTTg                                 >  1:253492/1‑140 (MQ=255)                                                                                                        ccAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGAt                            >  1:611441/1‑140 (MQ=255)                                                                                                          aaaGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGAttt                          >  1:97864/1‑140 (MQ=255)                                                                                                                 ttAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcgcg                   <  1:461188/140‑1 (MQ=255)                                                                                                                         aCGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTAGTGATTTCATCGCGGTTCTGGc           >  2:315380/1‑140 (MQ=255)                                                                                                                           gACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGGCTTGGGGGATTTCGTCGCGGTTCTGGCTg         >  1:222446/1‑140 (MQ=255)                                                                                                                                  cACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGGTCTGGAAACCGTATTTAGTGATTTCGTCGCAGTTCTGGCTGCCGCGCa  >  2:221784/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446445‑2446713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑