Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,455,256	T→C	T131A (ACC→GCC)	yfcV ←	putative fimbrial protein YfcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,455,256	0	T	C	100.0%	207.9 / NA	59	T131A (ACC→GCC)	yfcV	putative fimbrial protein YfcV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/33);  total (26/33)

ACCCAGATCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGTACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCTGAGT  >  NC_000913/2455125‑2455391                                                                                                                                    |                                                                                                                                        aCCCAGATCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCagag                                                                                                                                 >  2:532721/1‑140 (MQ=255)       aTCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATGCCCATGCGGCTTTTGTAAGAGGCACCCAGAGAGTCAc                                                                                                                           >  1:200084/1‑140 (MQ=255)       aTCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCAGCTGTTATAAGAGTCACCCAGAGAGTCAc                                                                                                                           >  1:253851/1‑140 (MQ=255)            gcgcATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATc                                                                                                                      >  1:24876/1‑140 (MQ=255)              gcATCAGCTGCGCCGACTAGCCTGGCTTTAACGTTCAGATTCTGCTTCTCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGc                                                                                                                    <  2:200084/140‑1 (MQ=255)              gcATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGc                                                                                                                    <  1:532721/140‑1 (MQ=255)                 tCAGCTGCGCCGACCAGCCTGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAg                                                                                                                 >  2:550318/1‑140 (MQ=255)                 tCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAg                                                                                                                 >  2:32446/1‑140 (MQ=255)                 tCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCAGCTCTTGTAAGAGGCAGCCAGAGAGTCACCGATCGCCAg                                                                                                                 >  2:277491/1‑140 (MQ=255)                        cgcCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACa                                                                                                          <  2:526553/140‑1 (MQ=255)                             aCCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGtt                                                                                                     >  1:738158/1‑140 (MQ=255)                                        tttAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCAc                                                                                          <  1:549089/140‑1 (MQ=255)                                             aGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggta                                                                                     <  2:33981/140‑1 (MQ=255)                                              gTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtat                                                                                    <  1:99452/140‑1 (MQ=255)                                              gTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtat                                                                                    <  1:97545/140‑1 (MQ=255)                                              gTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtat                                                                                    <  2:24876/140‑1 (MQ=255)                                              gTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtat                                                                                    <  1:550318/140‑1 (MQ=255)                                               ttCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtatc                                                                                   <  2:374525/140‑1 (MQ=255)                                                tCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtatcg                                                                                  <  2:33869/140‑1 (MQ=255)                                                 cAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtatcgg                                                                                 <  2:505549/140‑1 (MQ=255)                                                 cAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtatcgg                                                                                 >  1:702204/1‑140 (MQ=255)                                                 cAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtatcgg                                                                                 >  2:570631/1‑140 (MQ=255)                                                    agTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtatcggtat                                                                              >  2:603894/1‑140 (MQ=255)                                                      tCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGc                                                                                            <  1:690059/124‑1 (MQ=255)                                                      tCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGc                                                                                            >  2:690059/1‑124 (MQ=255)                                                          cTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCATGATCTTCTGGTCTATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGaaaa                                                                        <  2:253851/140‑1 (MQ=255)                                                          cTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGaaaa                                                                        <  1:572146/140‑1 (MQ=255)                                                          cTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGaaaa                                                                        <  2:702204/140‑1 (MQ=255)                                                          cTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGaaaa                                                                        <  1:95920/140‑1 (MQ=255)                                                                tttGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGgtgt                                                                  >  1:444535/1‑140 (MQ=255)                                                                    ccTTTATTAGTGGCAGTATCTTTCACGAATTTCTGGTCAATACACATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCACGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAACGGTGtaata                                                              >  2:704664/1‑140 (MQ=255)                                                                              tGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTGAATGCCGCACCGTTATTGGTATTGAAAATGTTGTAATAATTGCCGCTa                                                    >  2:299952/1‑140 (MQ=255)                                                                               ggCAGTATCTTTCACGATTTTCCGGCCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTAt                                                   <  1:277491/140‑1 (MQ=255)                                                                                 cAGTATCTTTCACGATTTTCTGGTCTATACCCATGCCCCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTAttt                                                 <  1:299952/140‑1 (MQ=255)                                                                                      tCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAACATGCTGTAATAATAGCCGCTATTTGCaga                                            >  1:253762/1‑140 (MQ=255)                                                                                      tCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTCCCGCTATTTGCaga                                            >  1:83261/1‑140 (MQ=255)                                                                                           cACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGaaa                                       <  2:6696/140‑1 (MQ=255)                                                                                           cACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGaaa                                       >  1:13210/1‑140 (MQ=255)                                                                                              gATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTAGCGGTATTGAAAACGGTCTAATAATTGCCGCCATTTGCAGAAGAAACgg                                    >  2:205927/1‑140 (MQ=255)                                                                                                ttttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTa                                  <  1:32446/140‑1 (MQ=255)                                                                                                ttttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTa                                  <  1:639280/140‑1 (MQ=255)                                                                                                ttttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGATGTTATAATTGCCGCTAGTTGCAGAAGAAACGGTa                                  >  2:277288/1‑140 (MQ=255)                                                                                                  ttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTAcc                                <  2:738158/140‑1 (MQ=255)                                                                                                  ttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTAcc                                >  2:49892/1‑140 (MQ=255)                                                                                                       gTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTaa                           <  1:110532/140‑1 (MQ=255)                                                                                                                 aTGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCGGCTATTTGCAGAAGAATCGGTTCCAGTAAAGGTAGTGGt                 >  2:292751/1‑140 (MQ=255)                                                                                                                  tGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTc                <  1:87492/140‑1 (MQ=255)                                                                                                                  tGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTc                >  1:31337/1‑140 (MQ=255)                                                                                                                      gCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTg            >  1:422900/1‑140 (MQ=255)                                                                                                                      gCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTg            >  1:382426/1‑140 (MQ=255)                                                                                                                      gCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTACTTGCAGAAAAAACGGTACCAGTGAAGGTAGTGGTCATTg            >  2:336938/1‑140 (MQ=255)                                                                                                                          ttGTAAGAGGCACCCGGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTc        <  1:198017/140‑1 (MQ=255)                                                                                                                           tGTAAGAGGCACCCAGAGAGTCTCCGATCTCCAGGCTGACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTcc       <  1:360844/140‑1 (MQ=255)                                                                                                                           tGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTcc       >  1:410314/1‑140 (MQ=255)                                                                                                                            gTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCt      <  2:410314/140‑1 (MQ=255)                                                                                                                            gTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCt      <  2:422900/140‑1 (MQ=255)                                                                                                                            gTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCt      <  2:444535/140‑1 (MQ=255)                                                                                                                              aaGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCTGa    <  1:570631/140‑1 (MQ=255)                                                                                                                                gagGCACCCAGAGAGCCACCGATCGTCAGACTAACATGGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCTGAGt  <  1:292751/140‑1 (MQ=255)                                                                                                                                 agGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCTGAGt  <  2:326980/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        ACCCAGATCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGTACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCTGAGT  >  NC_000913/2455125‑2455391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑