Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,457,177	T→C	G661G (GGA→GGG)	fadJ ←	3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,457,177	0	T	C	100.0%	104.0 / NA	31	G661G (GGA→GGG)	fadJ	3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

CAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCG  >  NC_000913/2457043‑2457313                                                                                                                                       |                                                                                                                                         cAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCAcc                                                                                                                                     >  1:362369/1‑140 (MQ=255)           ccccACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCCAGAAAAAATATATAGCAGAACAACCCACCGGGAAATGGc                                                                                                                           >  2:357465/1‑140 (MQ=255)                    cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCa                                                                                                                  <  2:413258/140‑1 (MQ=255)                    cgcCCATCTCGACCAAACGCTCCCATGGGCTAAAACGAGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCa                                                                                                                  <  1:199458/140‑1 (MQ=255)                                     cGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCa                                                                                                                  <  2:736315/123‑1 (MQ=255)                                     cGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCa                                                                                                                  >  1:736315/1‑123 (MQ=255)                                        tCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAat                                                                                              >  2:592238/1‑140 (MQ=255)                                           cAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATc                                                                                           <  2:411523/140‑1 (MQ=255)                                               gggTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGCGAGAATCGATATAGCGGAACGGCCCACCGAGAAAAGGCGGAACACCAATGCCAAATAACGCGCCAATATCCCCg                                                                                       >  2:328456/1‑140 (MQ=255)                                                      aCGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca                                                                                <  1:638737/140‑1 (MQ=255)                                                         ggAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCacgcacgc                                                                             <  2:625589/140‑1 (MQ=255)                                                                tACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGAt                                                                      >  2:718200/1‑140 (MQ=255)                                                                 aCTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATa                                                                     >  1:177602/1‑140 (MQ=255)                                                                     cGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCt                                                                 >  1:72310/1‑140 (MQ=255)                                                                     cGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCt                                                                 >  1:11493/1‑140 (MQ=255)                                                                      gTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTg                                                                <  1:519714/140‑1 (MQ=255)                                                                       tGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGc                                                               >  1:597395/1‑140 (MQ=255)                                                                             gTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCtcatca                                                         <  1:115657/140‑1 (MQ=255)                                                                                  tGCATTATTGCAACCCCTTCGCTCGAGCCCAGAGAATAGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAAcaca                                                    <  1:328456/140‑1 (MQ=255)                                                                                  tGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAAcaca                                                    <  2:565110/140‑1 (MQ=255)                                                                                            cAACCACTTCGCCCGGGCCGAGCGAATCGGTATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCt                                          <  1:264526/140‑1 (MQ=255)                                                                                            cAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCt                                          >  1:471963/1‑140 (MQ=255)                                                                                              aCCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttc                                        >  2:17335/1‑140 (MQ=255)                                                                                              aCCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttc                                        >  1:562852/1‑140 (MQ=255)                                                                                                 aCTTCGCCCGCGCCGATCGAATCGATATAGTCGCACGGCCCACCGAGAGATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcatt                                     <  2:351957/140‑1 (MQ=255)                                                                                                 aCTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcatt                                     >  1:587308/1‑140 (MQ=255)                                                                                                  cTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcattc                                    <  2:597395/140‑1 (MQ=255)                                                                                                   ttCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcattca                                   <  2:11493/140‑1 (MQ=255)                                                                                                   ttCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcattca                                   <  2:72310/140‑1 (MQ=255)                                                                                                     cGCCCGCGCCGAGAGAATCGATATAGCGGAACGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAGCCTGCTCATCAACACAAAGTACTGCTTCATTCAGc                                 >  2:249342/1‑140 (MQ=255)                                                                                                            gcCGAGAGAATCGATGTAGCGGAACCGCCCACCGAGATATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACa                          <  1:357465/140‑1 (MQ=255)                                                                                                                   gaATCGATATAGCGGAACGGCCCACCGAGAAATCGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATcacaca                   <  1:543545/140‑1 (MQ=255)                                                                                                                                  aaCGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtg    <  1:17335/140‑1 (MQ=255)                                                                                                                                    cGGCCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACCTACTGCTTCTTTCAGCATCAATATCACACACGGTTCAGCAACCtgcg  >  1:357235/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         CAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCG  >  NC_000913/2457043‑2457313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑