Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,057	T→C	S101S (AGT→AGC)	evgA →	DNA‑binding transcriptional activator EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,057	0	T	C	100.0%	183.6 / NA	52	S101S (AGT→AGC)	evgA	DNA‑binding transcriptional activator EvgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/18);  total (34/18)

AACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGTAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCAAAAACTCGACTCCTTATCGAAA  >  NC_000913/2483929‑2484192                                                                                                                                 |                                                                                                                                        aaCGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATCGTTTCGTGAGCAAAAAAGAAgg                                                                                                                              >  1:223179/1‑140 (MQ=255)  aCGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGc                                                                                                                             <  1:135908/140‑1 (MQ=255)  aCGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGc                                                                                                                             >  1:465335/1‑140 (MQ=255)   cGGTATCCAGGTGTTAGAAACGCTTAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCa                                                                                                                            <  2:13084/140‑1 (MQ=255)   cGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCa                                                                                                                            <  1:363579/140‑1 (MQ=255)           aGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAat                                                                                                                    <  2:411315/140‑1 (MQ=255)             gtgtTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAatat                                                                                                                  <  1:499988/140‑1 (MQ=255)                ttAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGc                                                                                                                             <  2:429058/126‑1 (MQ=255)                ttAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGc                                                                                                                             >  1:429058/1‑126 (MQ=255)                  aGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTg                                                                                                             >  1:697930/1‑140 (MQ=255)                          tGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAACAGAAGGCATGAACAATATCATTGCGGCTAtt                                                                                                     >  2:309771/1‑140 (MQ=255)                                        tataGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCaaaaaa                                                                                       >  2:593339/1‑140 (MQ=255)                                            gCGGAATTATTATTATCTTCTCCGCTAAAAATGACCATTATTACGGGACACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGc                                                                                   <  2:209413/140‑1 (MQ=255)                                               gAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTAc                                                                                >  1:37641/1‑140 (MQ=255)                                                aaTTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACt                                                                               >  2:718797/1‑140 (MQ=255)                                                  ttattattaTCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGc                                                                             >  2:676031/1‑140 (MQ=255)                                                   tattattatCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCt                                                                            <  2:223179/140‑1 (MQ=255)                                                          atCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTccc                                                                     >  2:363144/1‑140 (MQ=255)                                                          atCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTccc                                                                     >  2:710581/1‑140 (MQ=255)                                                          atCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTccc                                                                     >  2:533554/1‑140 (MQ=255)                                                          atCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTccc                                                                     <  1:718797/140‑1 (MQ=255)                                                           tCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTcccc                                                                    >  2:576832/1‑140 (MQ=255)                                                            cGTCTCCGCTAAAAATGACCATTTTTACGGGTATCTTTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCt                                                                   <  2:326890/140‑1 (MQ=255)                                                            cGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCt                                                                   <  1:676031/140‑1 (MQ=255)                                                              tctcCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAACAAATGGCTACTGCTATTTCCCCTtc                                                                 >  1:259133/1‑140 (MQ=255)                                                              tctcCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtc                                                                 >  1:723083/1‑140 (MQ=255)                                                              tctcCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtc                                                                 >  1:667914/1‑140 (MQ=255)                                                              tctcCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtc                                                                 >  2:659312/1‑140 (MQ=255)                                                               ctcCGCTAAAAATGACCGTTTTTACGGGAAATATTATGCCGATGCTGGTGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtct                                                                <  2:272189/140‑1 (MQ=255)                                                               ctcCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtct                                                                >  1:105635/1‑140 (MQ=255)                                                                tcCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAGATTGCTACTCCTATTTCCCCTtctc                                                               >  1:272189/1‑140 (MQ=255)                                                                tcCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtctc                                                               >  2:90562/1‑140 (MQ=255)                                                                  cGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAGGCTGCAAGAAAGGGATACTGCGATTTCCCCTtctctc                                                             >  1:194605/1‑140 (MQ=255)                                                                  cGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtctctc                                                             >  1:391048/1‑140 (MQ=255)                                                                  cGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTtctctc                                                             >  2:261136/1‑140 (MQ=255)                                                                                   tttACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGTTACCGCTGTTTCCCCTACTCTCTCAACCGGTTTGTTGGa                                            >  2:260506/1‑140 (MQ=255)                                                                                            aaCATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGATACTGCTATTTCACATAGTCTCTCAACCGGGATGTTGGAAGTTTAACg                                   >  2:353568/1‑140 (MQ=255)                                                                                            aaCATTGTGCTGATGCTGACGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACg                                   >  1:64873/1‑140 (MQ=255)                                                                                                 tgtgCTGATGCTGTCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGa                              >  2:391092/1‑140 (MQ=255)                                                                                                   tgCTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGAcc                            >  2:69084/1‑140 (MQ=255)                                                                                                   tgCTGATGCTGGCGCCAATGGTGTCCTGACCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGAcc                            <  1:263023/140‑1 (MQ=255)                                                                                                     cTGATGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTAGTTCCCCTTATCTTTCAGCCGGTTTGATGGAAGTTTAACGTCCGACCAg                          >  2:273737/1‑140 (MQ=255)                                                                                                        aTGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTGCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCaa                       >  2:293528/1‑140 (MQ=255)                                                                                                        aTGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGG‑TTGTTGGAAGTTTAACGTCCGACCAGCaaa                      >  2:580245/1‑140 (MQ=255)                                                                                                        aTGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCGCAACCGGGTTGTTGGAAGTTTCACGTCCGACCAGCaa                       >  1:290279/1‑140 (MQ=255)                                                                                                        aTGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCACAACCGGATTGCTGAAAGTTTAACGTCCGACCAGCaa                       >  1:233127/1‑140 (MQ=255)                                                                                                         tGCTGGCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCaaa                      <  2:465335/140‑1 (MQ=255)                                                                                                         tGCTGGCGCTAATGGGTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCaaa                      <  1:260506/140‑1 (MQ=255)                                                                                                             ggCGCTAATGGTTTCGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCAAAAACt                  >  2:599028/1‑140 (MQ=255)                                                                                                             ggCGCTAATGGTTTCGTGAACAAAACAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACGGGTTTGTAGGACGTCTAACGTACGACCAGCAAAAACt                  >  1:360102/1‑140 (MQ=255)                                                                                                                    aTGGTTTCGTGAGCATAAAAGACGGTATGGACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCAAAAACTCGACTcc           <  1:353568/140‑1 (MQ=255)                                                                                                                           cGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCAAAAACTCGACTCCTTATCGa    <  1:710581/140‑1 (MQ=255)                                                                                                                           cGTGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCAAAAACTCGACTCCTTATCGa    <  1:533554/140‑1 (MQ=255)                                                                                                                             tGAGCAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGGCCAGCAAAAACTCGTCTCCTTATCGaaa  <  2:667914/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        AACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGTAAAAAAGAAGGCATGAACAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTTTAACGTCCGACCAGCAAAAACTCGACTCCTTATCGAAA  >  NC_000913/2483929‑2484192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑