Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,496,075	G→A	G144E (GGA→GAA)	lpxP →	palmitoleoyl acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,496,075	0	G	A	100.0%	76.2 / NA	24	G144E (GGA→GAA)	lpxP	palmitoleoyl acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

ACAGTCGCGTACGTAAATGGTTTGATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGCGATGATCGGCAGAAATAATCTGCGCGGCATTGTCGGTGCAC  >  NC_000913/2495946‑2496209                                                                                                                                  |                                                                                                                                       aCAGTCGCGTACGTAAATGGTTTGATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGccaa                                                                                                                              >  1:574424/1‑140 (MQ=255) aCAGTCGCGTACGTAAATGGTTTGATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGccaa                                                                                                                              >  2:532427/1‑140 (MQ=255) aCAGTCGCGTACGTAAATGGTTTGATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGccaa                                                                                                                              >  1:568653/1‑140 (MQ=255)                      ttGATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGt                                                                                                         <  1:150014/140‑1 (MQ=255)                        gATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTcc                                                                                                       <  1:532427/140‑1 (MQ=255)                           gTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCcaca                                                                                                    <  2:628982/140‑1 (MQ=255)                              gAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGCTGGCTACCTATCGTCCACataa                                                                                                 >  1:316508/1‑140 (MQ=255)                                      ggATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGc                                                                                         >  2:61324/1‑140 (MQ=255)                                      ggATAACCTTAAACGCATACAAATGCAAAATCGCGCCGTTATGGTTGTGGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGc                                                                                         <  2:316508/140‑1 (MQ=255)                                            ccTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATgg                                                                                   >  1:752/1‑140 (MQ=255)                                             cTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGa                                                                                  >  2:703171/1‑140 (MQ=255)                                               tAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAAt                                                                                <  1:61324/140‑1 (MQ=255)                                                  aCGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATggg                                                                             >  2:698003/1‑140 (MQ=255)                                                            aTGCAAAATCGCGGCGTAATGGATGACGGCGTCCATTCTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCg                                                                   <  1:249398/140‑1 (MQ=255)                                                                    tCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCATACCCGTGGGCGCa                                                           >  2:679378/1‑140 (MQ=255)                                                                    tCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCa                                                           <  1:183302/140‑1 (MQ=255)                                                                       cggcgTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCGGCTCATGGAATGAGTGCAGACCCGTGGGCGCATgc                                                        >  2:238395/1‑140 (MQ=255)                                                                        agacagaTGGTTGTCGGCGTCCGTTGTATGTCGCTGGGACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCATgcg                                                       <  1:314711/134‑1 (MQ=255)                                                                                    gTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGc                                           >  2:362918/1‑140 (MQ=255)                                                                                    gTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAAAGGGTGCAAACCCGTAGGCGCATGCGCTCTAACAAAGc                                           >  1:328846/1‑140 (MQ=255)                                                                                         cGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGCTGGAATGGGTGCAGACACGTGGGCGAATGCCCTCTAACAAAGctgtct                                      >  2:314711/1‑135 (MQ=255)                                                                                           tCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGCGATGATc                                    <  2:752/140‑1 (MQ=255)                                                                                             cATTTTATGTCGCTGGTACTGGCCGGCCGCGCGTTTGAACTGTGCCAAGCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGCGATGATCgg                                  <  2:328846/140‑1 (MQ=255)                                                                                             cATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGCGATGATCgg                                  <  1:679378/140‑1 (MQ=255)                                                                                                                             gATGGAACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATTGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGCGATGATCGGCAGAAATAATCTGCGCGGCATTGTCGGTGCAc  <  2:574424/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       ACAGTCGCGTACGTAAATGGTTTGATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTGATGGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGCGATGATCGGCAGAAATAATCTGCGCGGCATTGTCGGTGCAC  >  NC_000913/2495946‑2496209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑