Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	333,796	A→G	V213A (GTG→GCG)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	333,796	0	A	G	100.0%	182.7 / NA	52	V213A (GTG→GCG)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/35);  total (17/35)

ATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCAAAAGCCCAGCGAATCGCGCC  >  NC_000913/333668‑333932                                                                                                                                 |                                                                                                                                         aTTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTttatta                                                                                                                               >  2:374333/1‑140 (MQ=255)   tGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAAt                                                                                                                             >  1:131035/1‑140 (MQ=255)   tGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAAt                                                                                                                             >  2:711375/1‑140 (MQ=255)     cGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTttatta                                                                                                                               <  1:689377/136‑1 (MQ=255)     cGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTttatta                                                                                                                               >  2:689377/1‑136 (MQ=255)      gTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGt                                                                                                                          >  1:409708/1‑140 (MQ=255)        cATCGCCTCACGGACCATGTAAACCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCGCTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTAt                                                                                                                        <  2:254687/140‑1 (MQ=255)          tCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATgc                                                                                                                      >  1:5483/1‑140 (MQ=255)           cGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATgcg                                                                                                                     <  1:182341/140‑1 (MQ=255)             ccTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGcgcg                                                                                                                   <  2:125360/140‑1 (MQ=255)             ccTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGcgcg                                                                                                                   <  1:374333/140‑1 (MQ=255)              cTCACGGACCATGTAATCCGGCAAGAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCgg                                                                                                                  <  1:594785/140‑1 (MQ=255)                  cGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtc                                                                                                              <  1:722538/140‑1 (MQ=255)                  cGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtc                                                                                                              <  2:71187/140‑1 (MQ=255)                    gACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAAGCAGCCCGATTTGTTATTAATCGTATGCGCGGTGtcct                                                                                                            >  1:200565/1‑140 (MQ=255)                     aCCATGTAATCCGGCAATAAACAAATTCCTTTACCTAAACTCTGGCACCGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           <  2:207089/140‑1 (MQ=255)                     aCCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           <  2:543372/140‑1 (MQ=255)                     aCCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           <  1:666458/140‑1 (MQ=255)                     aCCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           <  2:52851/140‑1 (MQ=255)                      ccATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctcc                                                                                                          <  1:577007/140‑1 (MQ=255)                        aTGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTccac                                                                                                        <  2:181145/140‑1 (MQ=255)                          gTAATCCGGCAAAAAGCCAATTTCTGCACCTAAAATCGGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTccacca                                                                                                      <  1:257118/140‑1 (MQ=255)                          gTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTccacca                                                                                                      <  1:607769/140‑1 (MQ=255)                          gTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTccacca                                                                                                      >  2:608149/1‑140 (MQ=255)                          gTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTccacca                                                                                                      <  1:98056/140‑1 (MQ=255)                          gTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGACCGTTTTTTTATTAATAGTATGCGCGGTGTCCTccacca                                                                                                      >  2:270780/1‑140 (MQ=255)                             aTCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCataa                                                                                                   <  2:395524/140‑1 (MQ=255)                               ccGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           >  1:508845/1‑130 (MQ=255)                               ccGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           <  2:508845/130‑1 (MQ=255)                               ccGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTGTTTATTAATCGTATACGCGGTGTCCTCCACCataata                                                                                                 >  1:207089/1‑140 (MQ=255)                                cGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAatat                                                                                                <  1:711375/140‑1 (MQ=255)                                   cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctcc                                                                                                          <  1:561749/127‑1 (MQ=255)                                   cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctcc                                                                                                          >  2:561749/1‑127 (MQ=255)                                   cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           >  1:147963/1‑126 (MQ=255)                                   cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGtcctc                                                                                                           <  2:147963/126‑1 (MQ=255)                                              ttCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGGCCTGCATCATAATGTTAGGGTAGAGACGc                                                                                  >  1:336467/1‑140 (MQ=255)                                                             tCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCg                                                                   <  2:5483/140‑1 (MQ=255)                                                              cTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGa                                                                  <  1:10862/140‑1 (MQ=255)                                                                ggCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATg                                                                <  1:37204/140‑1 (MQ=255)                                                                ggCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCACAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATg                                                                >  1:270367/1‑140 (MQ=255)                                                                 gCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGGCCTCCACCGTAAAATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATgg                                                               >  2:246938/1‑140 (MQ=255)                                                                      gACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTcc                                                          <  2:131438/140‑1 (MQ=255)                                                                            tGGTGTTGAAATCTGGCACCAGCATTGACTCCTGCCCGTACAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGcaca                                                    <  2:261876/140‑1 (MQ=255)                                                                            tGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGcaca                                                    <  2:415245/140‑1 (MQ=255)                                                                             ggTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACaa                                                   <  1:608149/140‑1 (MQ=255)                                                                                   gAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTa                                             <  1:128705/140‑1 (MQ=255)                                                                                                     tGACTCCTGCCCGTGTAGCAACTAGTCCGTTTTTTTATTAATCGTATGCGCGGTGTCCTCCTCCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGa                           <  1:339526/140‑1 (MQ=255)                                                                                                            tGCCCGTGCAGCAACCAGCGCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCaaa                    >  1:538998/1‑140 (MQ=255)                                                                                                            tGCCCGTGCAGCAACCAGCCCGCTTTTTTATTAATCGTATTCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCaaa                    >  2:475391/1‑140 (MQ=255)                                                                                                             gCCCGTGCAGCAACCAGCCCGCTTTTTTATTGATCGTATGCGCGGTGTTCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCaaaa                   <  2:268234/140‑1 (MQ=255)                                                                                                                        aaCCAGCCCGCTTTTTTATTAATCGTATTCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCAAAAGCCCAGCGAAt        <  1:475391/140‑1 (MQ=255)                                                                                                                             gCCCGCTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCAAAAGCCCAGCGAATCgcgc   <  2:409708/140‑1 (MQ=255)                                                                                                                              cccGCTTTTTTATTAATCGTATGCGTGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCAAAAGCCCAGCGAATCGCGcc  <  1:270780/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         ATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGTGATCCGGGGCGATAGCAAAAGCCCAGCGAATCGCGCC  >  NC_000913/333668‑333932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑