Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,500,410	T→C	F10L (TTC→CTC)	ypdB →	DNA‑binding transcriptional activator YpdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,500,410	0	T	C	100.0%	136.3 / NA	39	F10L (TTC→CTC)	ypdB	DNA‑binding transcriptional activator YpdB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/18);  total (21/18)

ATATCCGCCGCCTGGAGCCGGGGACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAATTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCATTTTTCTGGATATC  >  NC_000913/2500277‑2500544                                                                                                                                      |                                                                                                                                       atatCCGCCGCCTGGAGCCGGGGACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTgg                                                                                                                                  <  2:489567/140‑1 (MQ=255)  tatCCGCCGCCTGGAGCCGGGGACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGc                                                                                                                                 <  1:651019/140‑1 (MQ=255)     ccgccgCCTGGAGCCGGGGACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGcaca                                                                                                                              >  2:737227/1‑140 (MQ=255)                 gCCGGGGACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAg                                                                                                                  <  2:61010/140‑1 (MQ=255)                    ggggACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTg                                                                                                               <  1:737227/140‑1 (MQ=255)                      ggACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGTCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGc                                                                                                             <  2:79676/140‑1 (MQ=255)                                tGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAaga                                                                                                   >  2:571018/1‑140 (MQ=255)                                            tCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGAt                                                                                       >  1:390740/1‑140 (MQ=255)                                                 aCCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGgaga                                                                                  >  1:76194/1‑140 (MQ=255)                                                 aCCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGgaga                                                                                  >  2:115176/1‑140 (MQ=255)                                                      cGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAGCAGGAACTGAGCGGTCGAATTAAAGGGCACCACCAGATGGAGATTGTc                                                                             >  2:357329/1‑140 (MQ=255)                                                        cACCCCAGTCGCCTGACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCgg                                                                           >  2:450124/1‑140 (MQ=255)                                                        cACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGGACAGGCAGATGGAGATTGTCgg                                                                           >  2:304957/1‑140 (MQ=255)                                                         aCCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGc                                                                          <  2:303069/140‑1 (MQ=255)                                                           cccAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCAc                                                                        >  1:502704/1‑140 (MQ=255)                                                             cAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCt                                                                      >  2:11376/1‑140 (MQ=255)                                                                  gCCTCACAGGCTACGTTGTTGCCTTGAGCCGAAGTCATACTGTGAAAGCCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTgac                                                                 <  2:188106/140‑1 (MQ=255)                                                                   ccTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTgacg                                                                <  1:11376/140‑1 (MQ=255)                                                                    cTCACAGGCTACGTTTTTGCTTTGATCCGAAGTGATACTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTgacga                                                               <  1:269801/140‑1 (MQ=255)                                                                    cTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTgacga                                                               <  2:502704/140‑1 (MQ=255)                                                                    cTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTgacga                                                               <  2:367387/140‑1 (MQ=255)                                                                     tCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCGAGAGGGAGATTGTTGGCACCTTTgacgac                                                              >  1:209664/1‑140 (MQ=255)                                                                                     tGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGa                                              >  1:603753/1‑140 (MQ=255)                                                                                     tGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGa                                              >  2:362744/1‑140 (MQ=255)                                                                                         ttGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGtt                                          >  2:628622/1‑140 (MQ=255)                                                                                            aGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTTACGACGGTCTGGACGTGTTGAAGttttt                                       >  2:57268/1‑140 (MQ=255)                                                                                                      atatTGTGAAAGTCATCATTGTTGAAGACGACCTCGTGGGACAACAGGGACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAAc                             <  2:302564/140‑1 (MQ=255)                                                                                                      atatTGTGAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAAc                             >  1:34438/1‑140 (MQ=255)                                                                                                           gtgAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGt                        >  1:26779/1‑140 (MQ=255)                                                                                                            tgAAAGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTc                       >  1:56421/1‑140 (MQ=255)                                                                                                              aaaGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAAc                             <  1:554648/132‑1 (MQ=255)                                                                                                              aaaGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAAc                             >  2:554648/1‑132 (MQ=255)                                                                                                              aaaGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGa                     <  1:4233/140‑1 (MQ=255)                                                                                                              aaaGTCATCATTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTGTTTGCAGCATAACCGCGTCGa                     >  2:147119/1‑140 (MQ=255)                                                                                                                   catcatTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCa                <  2:56421/140‑1 (MQ=255)                                                                                                                     tcatTGTTGAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCAtt              <  2:308966/140‑1 (MQ=255)                                                                                                                        ttgttgAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCAttttt           <  1:716529/140‑1 (MQ=255)                                                                                                                          gttgAAGACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCATTTTTCt         <  1:436952/140‑1 (MQ=255)                                                                                                                                gACGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCATTTTTCTGGatat   >  2:143098/1‑140 (MQ=255)                                                                                                                                 aCGAACTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCATTTTTCTGGATATc  <  2:540831/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ATATCCGCCGCCTGGAGCCGGGGACGGAAATTGCGTTTTACATTCCTAACCAACGCACCCCAGTCGCCTCACAGGCTACGTTATTGCTTTGAGCCGGAGTGATATTGTGAAAGTCATCATTGTTGAAGACGAATTCCTGGCACAACAGGAACTGAGCTGGCTAATTAAAGAGCACAGCCAGATGGAGATTGTCGGCACCTTTGACGACGGTCTGGACGTGTTGAAGTTTTTGCAGCATAACCGCGTCGACGCCATTTTTCTGGATATC  >  NC_000913/2500277‑2500544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑