Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,515	C→T	H284Y (CAC→TAC)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,515	0	C	T	97.8%	138.1 / NA	46	H284Y (CAC→TAC)	talA	transaldolase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/29);  minor base A (1/0);  total (17/29)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGAC  >  NC_000913/2579381‑2579650                                                                                                                                       |                                                                                                                                        aTCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGCAGCGGAGTTCCGTTGGGAGTACAAt                                                                                                                                    >  1:357318/1‑140 (MQ=255) aTCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAAt                                                                                                                                    <  2:461725/140‑1 (MQ=255)   cGACTGACTATCGCACCGAATTCACCGAACGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCa                                                                                                                                  <  1:224804/140‑1 (MQ=255)                     aaTTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGa                                                                                                                >  2:638801/1‑140 (MQ=255)                     aaTTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGa                                                                                                                >  2:172951/1‑140 (MQ=255)                     aaTTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGGTCCGTTGGGAGAACGATCAGGATGCGATGGCGGTAGa                                                                                                                >  1:295131/1‑140 (MQ=255)                        ttACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGaaaa                                                                                                             <  2:715621/140‑1 (MQ=255)                         tACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGaaaaa                                                                                                            <  1:588959/140‑1 (MQ=255)                             gAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGt                                                                                                        >  2:596292/1‑140 (MQ=255)                               aGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGTGTACAATAAGGATG‑GATGGCGATAGAAAAACTGTCTg                                                                                                     >  1:306791/1‑140 (MQ=255)                               aGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGaaaaa                                                                                                            <  1:160351/134‑1 (MQ=255)                               aGGAGCTGCAGGAAAAAGTTTCGCCAGTGGCACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGaaaaa                                                                                                            >  2:160351/1‑134 (MQ=255)                                 gAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGa                                                                                                    <  1:588241/140‑1 (MQ=255)                                   gCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAg                                                                                                  <  2:514036/140‑1 (MQ=255)                                                     gCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCg                                                                                <  1:172951/140‑1 (MQ=255)                                                               cGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATTAACg                                                                      <  1:14695/140‑1 (MQ=255)                                                                    aTTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAAc                                                                 >  1:446975/1‑140 (MQ=255)                                                                    aTTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAAc                                                                 >  1:439801/1‑140 (MQ=255)                                                                     ttAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACt                                                                <  1:709366/140‑1 (MQ=255)                                                                              ccTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtct                                                       >  1:684096/1‑140 (MQ=255)                                                                              ccTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtct                                                       >  1:150362/1‑140 (MQ=255)                                                                                         gTTCCCACGCCCAGCTCCCATGTGCGAAGCGCAGTTCCGCTGGGAGTATAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa                                            <  2:223362/140‑1 (MQ=255)                                                                                         gTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa                                            <  2:439801/140‑1 (MQ=255)                                                                                         gTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa                                            <  2:125616/140‑1 (MQ=255)                                                                                         gTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa                                            <  2:161385/140‑1 (MQ=255)                                                                                            cccACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAAc                                         >  1:709086/1‑140 (MQ=255)                                                                                            cccACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAAc                                         >  1:524256/1‑140 (MQ=255)                                                                                            cccACGCCCAGCTCCCAGGAGCGAAGCGGAGATGCTTTGAGAGTACAAGCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAAc                                         <  2:347951/140‑1 (MQ=255)                                                                                                   ccAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAAc                                  <  2:524256/140‑1 (MQ=255)                                                                                                      gCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAg                               <  2:150362/140‑1 (MQ=255)                                                                                                         cccATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCa                            <  2:709086/140‑1 (MQ=255)                                                                                                         cccATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCa                            <  1:638801/140‑1 (MQ=255)                                                                                                          ccATGAGCGAAGCGGAGATCCGTTGGGAGTACAATCAGGGTG‑GATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACg                          <  2:306791/140‑1 (MQ=255)                                                                                                           cATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAATCTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACg                          <  2:13424/140‑1 (MQ=255)                                                                                                           cATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACg                          <  2:390586/140‑1 (MQ=255)                                                                                                           cATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACg                          <  2:470121/140‑1 (MQ=255)                                                                                                           cATGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACg                          <  2:684096/140‑1 (MQ=255)                                                                                                             tGAGCGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGa                        <  2:178215/140‑1 (MQ=255)                                                                                                                 cGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAgtgt                    >  2:436768/1‑140 (MQ=255)                                                                                                                 cGAAGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAgtgt                    >  1:704040/1‑140 (MQ=255)                                                                                                                    aGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTtat                 >  1:123942/1‑140 (MQ=255)                                                                                                                    aGCGGAGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATATTCGTGCAGCCAAACTATAAATCAGCCACGGAGTGTtat                 >  2:282120/1‑140 (MQ=255)                                                                                                                    aGCGGAGTTCCGTTGAGAGTACCATTAGGGTGCGATGCCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTtat                 <  1:271810/140‑1 (MQ=255)                                                                                                                         aGTTCCGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTTATATGTc            <  2:48182/140‑1 (MQ=255)                                                                                                                              cGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGaa       <  1:596292/140‑1 (MQ=255)                                                                                                                              cGTTGGGAGTACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGaa       <  2:704040/140‑1 (MQ=255)                                                                                                                                   ggAGTACAATCAGGATGCGATGGCGGTAGAAAAACAGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGAc  <  2:295131/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGAC  >  NC_000913/2579381‑2579650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑