Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,598,045	C→T	G274D (GGC→GAC)	bamC ←	outer membrane protein assembly factor BamC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,598,045	0	C	T	100.0%	147.1 / NA	44	G274D (GGC→GAC)	bamC	outer membrane protein assembly factor BamC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/17);  total (27/17)

AACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACG  >  NC_000913/2597913‑2598179                                                                                                                                     |                                                                                                                                       aaCTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGa                                                                                                                                 <  1:438617/140‑1 (MQ=255)           tgctgcGGTTATCTAAATCACCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTg                                                                                                                       >  2:422571/1‑140 (MQ=255)             ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTc                                                                                                                     >  2:25706/1‑140 (MQ=255)             ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGTGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTc                                                                                                                     >  1:61237/1‑140 (MQ=255)             ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTc                                                                                                                     >  1:372495/1‑140 (MQ=255)              tgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCg                                                                                                                    >  1:546045/1‑140 (MQ=255)                               ccAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAc                                                                                                   >  2:166891/1‑140 (MQ=255)                               ccAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAc                                                                                                   >  1:58449/1‑140 (MQ=255)                                cAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACt                                                                                                  >  2:433387/1‑140 (MQ=255)                                              taGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGcgccgc                                                                                    >  2:19932/1‑140 (MQ=255)                                                 tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGCACGGGTGCTGTCGGTCTCTTTCATGGCCACATTTTCCAGTGCCGCTgg                                                                                 >  2:351927/1‑140 (MQ=255)                                                    ccGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGCTCACTTTCATGACCTCTTTTTGCAGCGCCGCTGGCAg                                                                              >  1:292790/1‑140 (MQ=255)                                                     cGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGa                                                                             <  2:372495/140‑1 (MQ=255)                                                        aTGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGt                                                                          >  1:597093/1‑140 (MQ=255)                                                                         ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                         >  1:66142/1‑140 (MQ=255)                                                                         ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                         >  1:627542/1‑140 (MQ=255)                                                                         ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                         >  1:400450/1‑140 (MQ=255)                                                                         ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAACCCCGCTGGCAGACGATGCCAAACCACATTGaa                                                         >  2:320243/1‑140 (MQ=255)                                                                         ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCACGCCCACTTATTCCAGCCCCGCTGGCAGACGGTGCCAAACCACATTGaa                                                         >  2:225419/1‑140 (MQ=255)                                                                                  aGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCcgcg                                                <  1:422571/140‑1 (MQ=255)                                                                                    ttCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCAGGCAGACGTTGACACACCACAGTGAACGGCCCGCGTa                                              >  1:281951/1‑140 (MQ=255)                                                                                    ttCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGGGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTa                                              >  2:544903/1‑140 (MQ=255)                                                                                       cTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGa                                           <  1:19932/140‑1 (MQ=255)                                                                                       cTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGa                                           <  2:627542/140‑1 (MQ=255)                                                                                              tCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCAt                                    >  1:24754/1‑140 (MQ=255)                                                                                              tCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCAt                                    >  1:670074/1‑140 (MQ=255)                                                                                              tCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACCTTGACAAACCACATTGAACGGGCCGCGTACGACCAGCAt                                    >  2:304783/1‑140 (MQ=255)                                                                                                   gTCAGACAGCGGCTTATAAGTTCCGTCCATGTTGTCCCGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTa                               <  1:351927/140‑1 (MQ=255)                                                                                                     cAGACAGCGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTaaa                             <  2:546045/140‑1 (MQ=255)                                                                                                        aCAGCGGCTTATAAGGTACGGCAATTTTGTCCTGCGACCGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCg                          <  1:320243/140‑1 (MQ=255)                                                                                                            cGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCTAAACCACAGTGAGCGGACCGCGTACAACCATCATTGGTAAACCGgtgt                      >  2:206603/1‑140 (MQ=255)                                                                                                            cGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGgtgt                      >  2:74345/1‑140 (MQ=255)                                                                                                            cGGCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGgtgt                      >  1:470598/1‑140 (MQ=255)                                                                                                              gCTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTAGGAACAGCATTGGTAAACCGGTGtca                    >  1:335214/1‑140 (MQ=255)                                                                                                               cTTATAAGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGtcat                   <  2:58449/140‑1 (MQ=255)                                                                                                                  ataAGTTACGGCCATGTTGTCCCGCTAACCGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAg                <  1:206603/140‑1 (MQ=255)                                                                                                                    aaGTTACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCt              <  1:544903/140‑1 (MQ=255)                                                                                                                       ttACGGCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCa           <  1:25706/140‑1 (MQ=255)                                                                                                                          cGGCCATGTTGTCCTGCTAACGGGTGCTGTCGGTCACTTTCATGCCCATTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACtt        <  2:292790/140‑1 (MQ=255)                                                                                                                            gCCATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTg      >  1:112270/1‑140 (MQ=255)                                                                                                                             ccATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGt     <  2:670074/140‑1 (MQ=255)                                                                                                                              cATGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTa    <  1:132718/140‑1 (MQ=255)                                                                                                                               aTGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTAc   <  2:24754/140‑1 (MQ=255)                                                                                                                               aTGTTGTCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTAc   <  1:171458/140‑1 (MQ=255)                                                                                                                                tgttgtCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACg  >  1:510353/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       AACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACG  >  NC_000913/2597913‑2598179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑