Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,604,172	T→C	I109T (ATC→ACC)	hyfC →	hydrogenase 4 component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,604,172	0	T	C	100.0%	110.7 / NA	31	I109T (ATC→ACC)	hyfC	hydrogenase 4 component C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/14);  total (17/14)

TGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTATTAATCAGCAGCATGCTGGTGCTGGCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTATCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTG  >  NC_000913/2604035‑2604308                                                                                                                                          |                                                                                                                                         tGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTATTAATCAGCAGCATGCTGGTGCTGGCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTtacct                                                                                                                                        >  2:509230/1‑140 (MQ=255)       gACATCTTCAGGTCTGAAGTTCGGCCTGATGTCGTCGGTATTAATCAGTAGCATGCTGGTGCTGGCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTAtcttc                                                                                                                                  <  2:218844/140‑1 (MQ=255)                       aTGTTCCGCCTGATGCCGTGGGTATTAATCAGCAGCATGCTGGTGCTGGCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGttt                                                                                                                  >  1:588177/1‑140 (MQ=255)                                                 aaTCAGCAGCATGCTGGTGCTGGCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATa                                                                                        <  1:489663/140‑1 (MQ=255)                                                       cagcaTGCTGGTGCTGGCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGaa                                                                                  >  1:485412/1‑140 (MQ=255)                                                            tgctggtgctggCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGTGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCg                                                                             >  1:369985/1‑140 (MQ=255)                                                            tgctggtgctggCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCg                                                                             >  2:92509/1‑140 (MQ=255)                                                            tgctggtgctggCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCg                                                                             >  1:374278/1‑140 (MQ=255)                                                                 gtgctggCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGc                                                                        >  1:75395/1‑140 (MQ=255)                                                                       gCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGt                                                                  >  1:607781/1‑140 (MQ=255)                                                                           tGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCTGGAGTCGGt                                                              >  2:712912/1‑140 (MQ=255)                                                                             gCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGc                                                            <  2:391573/140‑1 (MQ=255)                                                                                       tGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATATCGGAAGTCCGTTTGTGGGAGTGGGTGCCAGTCGCGAg                                                  >  1:265221/1‑140 (MQ=255)                                                                                            attaCCGTTTCCCCTTTTGCGGACGCCGGCGATCTGACCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGAc                                             <  1:217558/140‑1 (MQ=255)                                                                                            attaCCGTTTCAGCTTTTGTGGGCGGCGGCGATCTGATATCCCTTTCCTATCTTCCTGAACTGTTTCGTTTTTTCTTTGCTCTTTCCGGTCTGGATACCGGAAGTTCGTTTGCGGGAGTCGGTGCCAGTCACGAGTTGAc                                             <  2:393089/140‑1 (MQ=255)                                                                                             ttaCCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTTGATACCGGGAGTGCGTCTGCGGGAGACGGTGCCAGTCGCGAGTTGACg                                            >  2:198187/1‑140 (MQ=255)                                                                                                     tCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCAt                                    >  1:88546/1‑140 (MQ=255)                                                                                                     tCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCAt                                    >  1:23399/1‑140 (MQ=255)                                                                                                     tCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCAt                                    <  2:23399/140‑1 (MQ=255)                                                                                                      ccccTTTTGCGGGCGGCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCAtt                                   <  2:607781/140‑1 (MQ=255)                                                                                                             tgtgggcggCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCg                            <  2:369985/137‑1 (MQ=255)                                                                                                               cgggcggCGGCGATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGaa                          >  1:152480/1‑140 (MQ=255)                                                                                                                  gcggcggcgATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCa                       <  2:662346/140‑1 (MQ=255)                                                                                                                   cggcggcgATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCaa                      <  2:485412/140‑1 (MQ=255)                                                                                                                          gATCTGATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTAt               <  1:92509/140‑1 (MQ=255)                                                                                                                               gATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATtctct          <  2:396067/140‑1 (MQ=255)                                                                                                                               gATCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATtctct          <  1:722831/140‑1 (MQ=255)                                                                                                                                aTCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATtctctc         >  2:16489/1‑140 (MQ=255)                                                                                                                                aTCACCCTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATtctctc         >  2:716973/1‑140 (MQ=255)                                                                                                                                    cccTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCActg     <  1:16489/140‑1 (MQ=255)                                                                                                                                     ccTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATtctct          <  2:491517/134‑1 (MQ=255)                                                                                                                                     ccTTACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATtctct          >  1:491517/1‑134 (MQ=255)                                                                                                                                       ttACCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCActgctg  >  1:475264/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTATTAATCAGCAGCATGCTGGTGCTGGCGATGGCCTTACCACTGTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCTTATCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGTCCGTTTGCGGGAGTCGGTGCCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCAATGCTTATTCTCTCACTGCTG  >  NC_000913/2604035‑2604308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑