Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,620,850	T→C	H8R (CAC→CGC)	upp ←	uracil phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,620,850	0	T	C	100.0%	85.6 / NA	25	H8R (CAC→CGC)	upp	uracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGA  >  NC_000913/2620715‑2620985                                                                                                                                        |                                                                                                                                        gTTACTTTTTCCGTTTCGAGGTCGGCGGCCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGttt                                                                                                                                     <  1:181176/140‑1 (MQ=255)       ttttCCGTTTCGAGGACGGCGGTCGCTTCGCAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGCAAGCGCTTGGTGCTGATAGCTTGCTCACGCATCAGTCCCAGCTTGTGGTTGACGAGTGGGCGTTTGACTTc                                                                                                                               >  2:715050/1‑140 (MQ=255)          tCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCAc                                                                                                                            <  1:36591/140‑1 (MQ=255)                tCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCtt                                                                                                                      >  2:399397/1‑140 (MQ=255)                   aGGTCGGCTGTCGCTTCATAAGACAGCAGGCAAGCCACTACGGAAGCGGGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCAt                                                                                                                   <  2:326452/140‑1 (MQ=255)                                             cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCGACGATCTGCATGCTATTTCTCTTTTAAGGGGCAGCca                                                                                         >  1:204296/1‑140 (MQ=255)                                                         ttCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCg                                                                             <  1:715050/140‑1 (MQ=255)                                                          tCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGa                                                                            <  1:53549/140‑1 (MQ=255)                                                          tCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGa                                                                            <  1:399397/140‑1 (MQ=255)                                                                 cGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATta                                                                     <  2:535094/140‑1 (MQ=255)                                                                   aGTTCGGGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGa                                                                            <  1:243871/131‑1 (MQ=255)                                                                   aGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGa                                                                            >  2:243871/1‑131 (MQ=255)                                                                     ttCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATCCTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcc                                                                 <  2:702236/140‑1 (MQ=255)                                                                     ttCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcc                                                                 <  2:440264/140‑1 (MQ=255)                                                                        gcgAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                                                              >  2:530891/1‑140 (MQ=255)                                                                                               cTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCt                                       <  1:530891/140‑1 (MQ=255)                                                                                                ttGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt                                      >  1:696578/1‑140 (MQ=255)                                                                                                ttGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt                                      >  1:532735/1‑140 (MQ=255)                                                                                                  gCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGAGGGATTATACCTCCTTTCTTCAGGGCGGCAATATTCtttt                                    >  2:315980/1‑140 (MQ=255)                                                                                                     cACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGt                                 >  2:261868/1‑140 (MQ=255)                                                                                                                       tgtgTTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTATCGTTGACTTTAGTCAAAATGa               >  1:276649/1‑140 (MQ=255)                                                                                                                         tgtTTGACGAGTGGGCGTTTGAGTTCCACGAACTTCATATTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATa             <  2:276649/140‑1 (MQ=255)                                                                                                                         tgtTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATa             >  2:635304/1‑140 (MQ=255)                                                                                                                         tgtTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATa             >  1:431948/1‑140 (MQ=255)                                                                                                                          gtTTGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATaa            <  2:696578/140‑1 (MQ=255)                                                                                                                           tttGACGAGTGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAAc           >  1:559488/1‑140 (MQ=255)                                                                                                                                    tGGGCGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTgaga  <  1:736478/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGA  >  NC_000913/2620715‑2620985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑