Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,973	A→G	G366G (GGT→GGC)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,973	0	A	G	100.0%	102.2 / NA	31	G366G (GGT→GGC)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/11);  total (20/11)

AGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAA  >  NC_000913/2632836‑2633109                                                                                                                                          |                                                                                                                                         aGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGcc                                                                                                                                        >  1:701195/1‑140 (MQ=255)    ttGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCCGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGc                                                                                                                                     <  2:462223/140‑1 (MQ=255)    ttGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCCCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGCGCCCGc                                                                                                                                     <  2:444949/140‑1 (MQ=255)                           gAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACcg                                                                                                              >  1:144179/1‑140 (MQ=255)                                     tCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGcc                                                                                                    >  2:23140/1‑140 (MQ=255)                                       agagGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCTGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAg                                                                                                  >  2:514873/1‑140 (MQ=255)                                            aaCCTTTGGACATCGCTCCCAGGGAACCCATACCACGGTAAGATTTGTAAGACCGGCCCGGGTAGATTTCGATTTCACCCGGAGATTCTGCATTGCCCGCCAGCATGGAACCTTCCATCACCGCGCTTGCGCCAgcggcg                                                                                             <  1:664870/140‑1 (MQ=255)                                             aCCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCTGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGa                                                                                            <  1:514873/140‑1 (MQ=255)                                                 ttGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCACGCCAGCATGGATCCTACCATCACCACGCTGGCGCCAGCGGCGATAGc                                                                                        >  2:275156/1‑140 (MQ=255)                                                                      cccATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGaa                                                                   >  2:645658/1‑140 (MQ=255)                                                                      cccATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGaa                                                                   >  2:153016/1‑140 (MQ=255)                                                                          tACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGa                                                               >  1:626259/1‑140 (MQ=255)                                                                                ggTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCg                                                         >  2:460893/1‑140 (MQ=255)                                                                                ggTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCg                                                         >  1:371746/1‑140 (MQ=255)                                                                                ggTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGAGATAGCTGTGGAGAGGTCGCCGGAGAAGCGAATACCg                                                         >  1:203982/1‑140 (MQ=255)                                                                                 gTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGc                                                        >  2:688211/1‑140 (MQ=255)                                                                                     gATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATc                                                    >  2:505727/1‑140 (MQ=255)                                                                                     gATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATc                                                    >  2:114012/1‑140 (MQ=255)                                                                                                 cGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCgg                                        >  2:115768/1‑140 (MQ=255)                                                                                                    cccTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAAt                                     >  2:701083/1‑140 (MQ=255)                                                                                                      cTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATAc                                   <  2:62424/140‑1 (MQ=255)                                                                                                              gTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCATCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCt                           >  1:485562/1‑140 (MQ=255)                                                                                                              gTTCGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCt                           >  1:660451/1‑140 (MQ=255)                                                                                                                 cGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTcc                        <  1:688211/140‑1 (MQ=255)                                                                                                                 cGATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTcc                        <  1:153016/140‑1 (MQ=255)                                                                                                                  gATTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCa                       <  1:23140/140‑1 (MQ=255)                                                                                                                   aTTTCACCCGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAg                      >  2:512398/1‑140 (MQ=255)                                                                                                                           cGGAGATTCTTCAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTa              <  1:115768/140‑1 (MQ=255)                                                                                                                                  tcttcAGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTc       >  2:450039/1‑140 (MQ=255)                                                                                                                                       aGTGCCCGCCAGCATTGAACCTACCCTCGTCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCaa  <  1:275156/140‑1 (MQ=255)                                                                                                                                       aGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCaa  <  2:144179/140‑1 (MQ=255)                                                                                                                                       aGTGCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCaa  <  1:450039/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAA  >  NC_000913/2632836‑2633109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑