Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,663,078	A→G	R82R (CGT→CGC)	trmJ ←	tRNA Cm32/Um32 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,663,078	0	A	G	100.0%	83.0 / NA	24	R82R (CGT→CGC)	trmJ	tRNA Cm32/Um32 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/10);  total (14/10)

TTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAACGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGT  >  NC_000913/2662943‑2663210                                                                                                                                        |                                                                                                                                     tttCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTg                                                                                                                                  >  1:541225/1‑140 (MQ=255) tttCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTg                                                                                                                                  <  2:479628/140‑1 (MQ=255)       cAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTgg                                                                                                                            <  2:476377/140‑1 (MQ=255)             ttCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCcca                                                                                                                      >  2:626423/1‑140 (MQ=255)             ttCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCcca                                                                                                                      >  1:683008/1‑140 (MQ=255)                         gCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTAc                                                                                                          >  2:449544/1‑140 (MQ=255)                              cgcgCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCg                                                                                                     >  1:696715/1‑140 (MQ=255)                                       gACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCt                                                                                            <  1:387168/140‑1 (MQ=255)                                         ccAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTtc                                                                                          <  2:683008/140‑1 (MQ=255)                                                 cAGGGCCACCGGGGTATTTGCCCCTTCGGCAACGCTATGCAGGCTGCATGCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGCGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCg                                                                                  <  2:140130/140‑1 (MQ=255)                                                   gcgcCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTa                                                                                >  2:500508/1‑140 (MQ=255)                                                   gcgcCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTa                                                                                <  2:21299/140‑1 (MQ=255)                                                      ccACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATcg                                                                             >  1:540587/1‑140 (MQ=255)                                                        aCCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACACCCGGCTCACGCTTTGTCGAGCGTATcgac                                                                           >  1:256236/1‑140 (MQ=255)                                                                aTTTGCCGCTTCAGCGACGCTTGTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAg                                                                   <  2:275111/140‑1 (MQ=255)                                                                       gCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTCACGCTTCGTCGAGCGTATCGAGGATGTGAGCATTAcc                                                            >  1:247369/1‑140 (MQ=255)                                                                         ttCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCaa                                                          >  1:92025/1‑140 (MQ=255)                                                                                          ggCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCct                                         <  2:696715/140‑1 (MQ=255)                                                                                                aTTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGcc                                   >  2:59279/1‑140 (MQ=255)                                                                                                       cgGGTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCaa                            <  1:500508/140‑1 (MQ=255)                                                                                                          gTCGAGCATCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCg                         >  1:612464/1‑140 (MQ=255)                                                                                                            cGAGCATCGGCCATGGCAGCGTGCGGGAGCGGGCGTTGGAGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGcc                       <  2:256236/140‑1 (MQ=255)                                                                                                                  tCGGCCATGGCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATGACATCGCAGGCCCCTGGTGCCAGGGCAATCGCCTGGGAg                 >  2:247124/1‑140 (MQ=255)                                                                                                                          ggCAGCGTGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGttt         <  2:92025/140‑1 (MQ=255)                                                                                                                                 tGCGGGAGCGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGt  >  2:96967/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     TTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACGCTTTTCAGGCCGCATTCGCGCGGGTCGAGCATCGGCCATGGCAGCGTGCGGGAACGTGCGCTGGTGCCCACCACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGT  >  NC_000913/2662943‑2663210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑