Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,690,350	C→A	R250L (CGC→CTC)	glrK ←	sensory histidine kinase GlrK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,690,350	0	C	A	100.0%	144.7 / NA	44	R250L (CGC→CTC)	glrK	sensory histidine kinase GlrK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/21);  total (23/21)

AGAATGCTCACCACCTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCT  >  NC_000913/2690218‑2690485                                                                                                                                     |                                                                                                                                        aGAATGCTCACCACCTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGa                                                                                                                                  <  1:142954/140‑1 (MQ=255)   aaTGCTCACCACCTCTTTTTGCTCTCGCGTAAGCGGCACGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGAtt                                                                                                                                <  1:298326/140‑1 (MQ=255)      gCTCACCACCTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGGGATAAATGTCTGAATAATTGAGGGAGTTGGGATTCCa                                                                                                                             >  1:202514/1‑140 (MQ=255)      gCTCACCACCTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCa                                                                                                                             <  1:25597/140‑1 (MQ=255)       cTCACCACCTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAg                                                                                                                            >  2:407571/1‑140 (MQ=255)          accaccTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCa                                                                                                                         >  1:655025/1‑140 (MQ=255)          accaccTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCa                                                                                                                         >  1:464992/1‑140 (MQ=255)           ccaccTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCAt                                                                                                                        <  2:729136/140‑1 (MQ=255)            caccTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGCGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATg                                                                                                                       >  2:110201/1‑140 (MQ=255)             accTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGa                                                                                                                      >  1:59262/1‑140 (MQ=255)              ccTCTTTTTGCTCTGACGTAAGCGGCCCGCCAACCTGCTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGAc                                                                                                                     <  1:196616/140‑1 (MQ=255)                      tGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTc                                                                                                             >  2:10345/1‑140 (MQ=255)                         tctGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACt                                                                                                          <  1:407571/140‑1 (MQ=255)                         tctGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACt                                                                                                          <  2:642417/140‑1 (MQ=255)                         tctGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACt                                                                                                          >  2:482027/1‑140 (MQ=255)                          ctGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACtt                                                                                                         >  1:714724/1‑140 (MQ=255)                             gCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAAc                                                                                                      <  2:714724/140‑1 (MQ=255)                                       cccGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATAc                                                                                            >  1:610178/1‑140 (MQ=255)                                                 tGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAACTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGGTTCCAGCCATGATAGGTGCTCACTTAACCAAAGAATACGTTGCCCAAc                                                                                  >  2:273058/1‑140 (MQ=255)                                                    tCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTGCAGCCATGACAGCCGCCCACTTAACCAAAGAATACGTTGCCCAACCGa                                                                               >  2:326841/1‑140 (MQ=255)                                                     cAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAg                                                                              >  1:500924/1‑140 (MQ=255)                                                     cAGCCAGTAATTCAGCGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAg                                                                              <  1:110201/140‑1 (MQ=255)                                                      aGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGc                                                                             <  2:59262/140‑1 (MQ=255)                                                         cAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTa                                                                          >  1:68509/1‑140 (MQ=255)                                                           gTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAAc                                                                        <  2:500924/140‑1 (MQ=255)                                                             aaTTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActc                                                                      <  1:574354/140‑1 (MQ=255)                                                                tCAGTGCCCTCGCGCCTACTCGCCACTGGGGTTTTTAACTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgct                                                                   <  1:273058/140‑1 (MQ=255)                                                                tCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgct                                                                   <  2:655025/140‑1 (MQ=255)                                                                tCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgct                                                                   <  2:464992/140‑1 (MQ=255)                                                                  aGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgctcg                                                                 >  1:582549/1‑140 (MQ=255)                                                                         tCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACt                                                          >  1:423486/1‑140 (MQ=255)                                                                            cgcATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGaa                                                       <  1:10345/140‑1 (MQ=255)                                                                                tACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACgag                                                   >  2:659161/1‑140 (MQ=255)                                                                                 aCTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACgaga                                                  <  2:582549/140‑1 (MQ=255)                                                                                                      ttCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGcc                             <  2:99695/140‑1 (MQ=255)                                                                                                       tCATGAGATAAATGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGccc                            <  1:482027/140‑1 (MQ=255)                                                                                                                  aTGTCTTAAAAATTGCTGGAGTCGGAATTGCAGCCATTACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAc                 <  1:229677/140‑1 (MQ=255)                                                                                                                   tGTCTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACg                >  1:710066/1‑140 (MQ=255)                                                                                                                      cTTAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCACTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGtt             >  1:458624/1‑140 (MQ=255)                                                                                                                       ttAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGTGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTg            >  1:628240/1‑140 (MQ=255)                                                                                                                       ttAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTg            >  2:631274/1‑140 (MQ=255)                                                                                                                       ttAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTg            >  1:703320/1‑140 (MQ=255)                                                                                                                        tAAAAATTGATGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGa           >  2:558291/1‑140 (MQ=255)                                                                                                                                 aTGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCt  >  1:605129/1‑140 (MQ=255)                                                                                                                                 aTGGAGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCACTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCt  <  2:458624/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        AGAATGCTCACCACCTCTTTTTGCTCTGGCGTAAGCGGCCCGACAACCTGGTCAGCCAGTAATTCAGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCT  >  NC_000913/2690218‑2690485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑