Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	359,829	A→G	E114G (GAG→GGG)	cynS →	cyanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	359,829	0	A	G	100.0%	150.7 / NA	43	E114G (GAG→GGG)	cynS	cyanase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/16);  total (27/16)

CATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGAGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCGTTCTGACA  >  NC_000913/359701‑359961                                                                                                                                 |                                                                                                                                     cATTCTACTGTTGCACATGATTCCATTGCGTGGCTGCCTTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCg                                                                                                                           <  1:193003/140‑1 (MQ=255)   ttCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGAt                                                                                                                         <  1:3233/140‑1 (MQ=255)     cTACTGTTGCAGATGCTTCCACGGCATGGTTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATgg                                                                                                                       <  1:330259/140‑1 (MQ=255)       aCTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCg                                                                                                                           >  1:124924/1‑134 (MQ=255)       aCTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCg                                                                                                                           <  2:124924/134‑1 (MQ=255)       aCTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCa                                                                                                                     >  2:96104/1‑140 (MQ=255)       aCTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCa                                                                                                                     >  1:508000/1‑140 (MQ=255)         tGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCatt                                                                                                                   >  1:45440/1‑140 (MQ=255)              cAGATGATTCCACTGCGTGGCTACATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAg                                                                                                              >  2:56247/1‑140 (MQ=255)                     ttCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGAtt                                                                                                       >  2:469197/1‑140 (MQ=255)                     ttCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGAtt                                                                                                       >  1:547193/1‑140 (MQ=255)                         aCTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACt                                                                                                   <  1:469197/140‑1 (MQ=255)                               tGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGAtt                                                                                                       >  2:591260/1‑130 (MQ=255)                               tGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGAtt                                                                                                       <  1:591260/130‑1 (MQ=255)                               tGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAAc                                                                                             >  1:602798/1‑140 (MQ=255)                                  cTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCg                                                                                          <  2:669696/140‑1 (MQ=255)                                     cATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACg                                                                                       >  2:484364/1‑140 (MQ=255)                                               cGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGt                                                                             >  1:481924/1‑140 (MQ=255)                                                   ttCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCg                                                                         <  1:107913/140‑1 (MQ=255)                                                            aCCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATAAGCGCGATTAACTTCACACTCGACGGTAAGAAAGTGGCGGACCCGGaa                                                                >  2:288808/1‑140 (MQ=255)                                                                      gTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAAc                                                      >  1:383373/1‑140 (MQ=255)                                                                       tATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACg                                                     >  1:157703/1‑140 (MQ=255)                                                                            tttCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCgg                                                >  1:555564/1‑140 (MQ=255)                                                                              tCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGtc                                              >  1:691843/1‑140 (MQ=255)                                                                              tCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGtc                                              >  2:44196/1‑140 (MQ=255)                                                                                 aTGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAGAGTGGCGGACCGGGGAGGTGGTGAACGTGCGGtcaac                                           >  1:278128/1‑138 (MQ=255)                                                                                 aTGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGtcatc                                           <  2:45440/140‑1 (MQ=255)                                                                                 aTGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGtcatc                                           <  2:602798/140‑1 (MQ=255)                                                                                       tGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTa                                     <  2:508000/140‑1 (MQ=255)                                                                                         ttGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGa                                   <  2:383373/140‑1 (MQ=255)                                                                                            cAGGTGTACGGTACACCCCTGAAAGTGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATgg                                <  1:288808/140‑1 (MQ=255)                                                                                                    cGGTACAACCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATc                        >  2:738987/1‑140 (MQ=255)                                                                                                          aaCCCTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGa                  >  1:4048/1‑140 (MQ=255)                                                                                                            cccTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGAACCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGAT‑GTAAATATCTGCCGACCa               >  1:8627/1‑140 (MQ=255)                                                                                                              cTGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCaa              <  2:691843/140‑1 (MQ=255)                                                                                                               tGAAAGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGAGGCGAACGAGCGGTCATCACCTTAGGTGGTAAATATCTGCCGACCaaa             >  2:295324/1‑140 (MQ=255)                                                                                                                 aaaGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTTATCATCTTAGATGGTAAAGATCTGCCGACCAAAcc           >  2:340137/1‑140 (MQ=255)                                                                                                                 aaaGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAAcc           >  1:502590/1‑140 (MQ=255)                                                                                                                 aaaGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAAcc           >  1:498176/1‑140 (MQ=255)                                                                                                                  aaGCGTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCg          <  1:484364/140‑1 (MQ=255)                                                                                                                      gTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCGTTCt      >  2:29184/1‑140 (MQ=255)                                                                                                                      gTTGGTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCGTTCt      >  1:41319/1‑140 (MQ=255)                                                                                                                          gTTCATGGGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCGTTCTGACa  <  1:29184/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     CATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATGTTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGAGAAATTTGGCGATGGCATTATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAACGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCGTTCTGACA  >  NC_000913/359701‑359961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑