Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,771,040	G→C	T120T (ACG→ACC)	yfjS →	CP4‑57 prophage; inner membrane lipoprotein YfjS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,771,040	0	G	C	100.0%	16.8 / NA	11	T120T (ACG→ACC)	yfjS	CP4‑57 prophage; inner membrane lipoprotein YfjS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (6/5);  total (6/5)

GACAATATCAAACTGGAATACCACGAAGTTACGGACCCGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTT  >  NC_000913/2770996‑2771174                                             |                                                                                                                                       gataacatCAAACTGGAATACCTGGAGGTTACGGACCTGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACgaga                                         <  1:255433/134‑1 (MQ=14)  ataacatCAAACTGGAATACCAGGAGGTGACGGACCCGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGCCAAACCCTGATACCCGGAAATAAAAACAACGAGaa                                        >  2:297386/6‑140 (MQ=11)           aaCTGGAATACCAGGAGGTGACGGACCCGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACAt                               <  2:535072/140‑1 (MQ=14)              tGGAATACCAGGAGGTGACGGACCCGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATaaa                            >  1:472993/1‑140 (MQ=11)                    aCCAGGAGGTGACGGACCCGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGaaaa                      <  2:154615/140‑1 (MQ=17)                          aggtgaCGGACCCGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGtt                >  2:701235/6‑140 (MQ=14)                               aCGGACCCGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATaaa                            <  1:722247/123‑1 (MQ=18)                               aCGGACCCGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATaaa                            >  2:722247/1‑123 (MQ=14)                                    cccGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCg      >  2:25691/1‑140 (MQ=17)                                      cGTATACCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGt    <  1:576283/140‑1 (MQ=18)                                        tataCCAAAGAAAAATACTTTGAGTACGCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACAGAAAAGAAAATACGCTCACTAAGCCGGttt  >  1:283700/1‑140 (MQ=17)                                             |                                                                                                                                       GACAATATCAAACTGGAATACCACGAAGTTACGGACCCGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTT  >  NC_000913/2770996‑2771174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑