Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,807,382	T→C	V84A (GTG→GCG)	proX →	glycine betaine ABC transporter periplasmic binding protein ProX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,807,382	0	T	C	100.0%	126.3 / NA	36	V84A (GTG→GCG)	proX	glycine betaine ABC transporter periplasmic binding protein ProX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/15);  total (21/15)

CTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACA  >  NC_000913/2807247‑2807513                                                                                                                                        |                                                                                                                                    cTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAAc                                                                                                                                 >  1:685066/1‑140 (MQ=255)         aCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACAGCTCGCTTGTTTCAGGCGATGCAACCCTCACAGCCGCGAACTGGTCGcc                                                                                                                         >  2:219514/1‑140 (MQ=255)          ccTCCCAGACGCTGATGGTCAGTCGTGCGCTGGAGAAATTAGGTTATCCCGCCAAAAACCCCAGCAAAGTAGATTACAACGTTGGCTACGCCTCGCTTGCTTCCGGCGATCCAACTTTCGCCGCCGCGAACTGGACGCCa                                                                                                                        <  2:79938/140‑1 (MQ=255)              ccAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGGTGCAACCTTCACTGCCGCGAACTGGACGCCACTGc                                                                                                                    >  2:308109/1‑140 (MQ=255)                 gACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATg                                                                                                                 <  1:485452/140‑1 (MQ=255)                  ccGCTGCTGGTCAGTCGTGCGCAGGCGAATTTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACCCCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGa                                                                                                                <  1:344081/139‑1 (MQ=255)                   cGCTGCTGGTCAGTCGTCCGCTGGAGAAATTTGGTTATACCGTCAACAACCCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGac                                                                                                               <  2:287392/140‑1 (MQ=255)                     ctgctgGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGacaa                                                                                                             >  2:40164/1‑140 (MQ=255)                      tgctgGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCATCATAGCCGCGATCTGGGGGCCACTGTATGacaac                                                                                                            >  2:344081/1‑140 (MQ=255)                                          gagaAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGTCGCCACTTCATGACGACATGTACGAAGCTGCCGGTgg                                                                                        >  1:214501/1‑140 (MQ=255)                                               aTTAGGTTATACCGTTAACAAACCCTGCGCAGTAGATGACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGTCGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATa                                                                                   <  1:219514/140‑1 (MQ=255)                                                        taCCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAAtttt                                                                          >  2:577365/1‑140 (MQ=255)                                                             tCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGt                                                                     >  2:80110/1‑140 (MQ=255)                                                             tCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGt                                                                     >  2:481866/1‑140 (MQ=255)                                                              caacaaACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTg                                                                    >  2:379606/1‑140 (MQ=255)                                                               aacaaACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGa                                                                   <  1:643975/140‑1 (MQ=255)                                                                 caaACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAg                                                                 <  1:577365/140‑1 (MQ=255)                                                                    ccccAGCGAAGTAGATTTCAACCTTGGCTACCCCTAGCTCGCTTCCGGGGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAgggg                                                              <  2:214501/139‑1 (MQ=255)                                                                                  aTTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc                                                >  2:110683/1‑140 (MQ=255)                                                                                   ttACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcg                                               <  1:110683/140‑1 (MQ=255)                                                                                    tACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc                                              >  1:524150/1‑140 (MQ=255)                                                                                      cAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcg                                               >  1:53862/1‑137 (MQ=255)                                                                                      cAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcg                                               <  2:53862/137‑1 (MQ=255)                                                                                        aCGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGca                                          <  1:40164/140‑1 (MQ=255)                                                                                        aCGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGca                                          <  1:379606/140‑1 (MQ=255)                                                                                           ttGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGAGATAAGAAATTTAATCCTGAAGGGGTATTTGTTAACGGCGCGGCACAg                                       >  2:238810/1‑140 (MQ=255)                                                                                            tGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGcgcgac                                           <  1:650916/135‑3 (MQ=255)                                                                                            tGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGcgcgac                                           >  2:650916/1‑133 (MQ=255)                                                                                            tGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAgg                                      >  1:175782/1‑140 (MQ=255)                                                                                            tGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAAATTTATCGAGAAGGGGTTTTTGTTAACGGCGCGGCACAgg                                      >  2:287226/1‑140 (MQ=255)                                                                                                        gcttgcttCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGca                                          <  1:636086/124‑1 (MQ=255)                                                                                                        gcttgcttCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGca                                          >  2:636086/1‑124 (MQ=255)                                                                                                        gcttgcttCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCg                          >  1:121925/1‑140 (MQ=255)                                                                                                               tCCGGCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGaa                   >  2:162036/1‑140 (MQ=255)                                                                                                                  ggCGATGCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAc                >  2:549463/1‑140 (MQ=255)                                                                                                                        gCAACCTTCACCGCCGCGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGa          <  1:80110/140‑1 (MQ=255)                                                                                                                         cAACCTCCACCGCCGCGAACTGCACCCCATTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGAc         <  1:212543/140‑1 (MQ=255)                                                                                                                                cACCGCCGCGAACTGTACGCCAATGTATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACa  <  1:308109/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACA  >  NC_000913/2807247‑2807513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑