Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,852,654	C→T	C173C (TGC→TGT)	hypD →	Fe‑(CN)2CO cofactor assembly scaffold protein HypD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,852,654	0	C	T	100.0%	96.3 / NA	28	C173C (TGC→TGT)	hypD	Fe‑(CN)2CO cofactor assembly scaffold protein HypD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/11);  total (17/11)

TGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGC  >  NC_000913/2852527‑2852783                                                                                                                                |                                                                                                                                  tGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACg                                                                                                                       <  1:397347/140‑1 (MQ=255)  ggCGCAGGAGAATCCAGTCCGCAAAGTGGTGTTTTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGc                                                                                                                      <  2:316061/140‑1 (MQ=255)   gcgcAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCt                                                                                                                     <  2:681308/140‑1 (MQ=255)    cgcAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCtt                                                                                                                    >  2:501250/1‑140 (MQ=255)             aTCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACg                                                                                                           >  2:644676/1‑140 (MQ=255)                   cccGCAAAGTGGTGTTCCTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTgcgc                                                                                                     >  2:503973/1‑140 (MQ=255)                            tggtgTTCTTCGGCTTAGGTTTTGAAACCAGTATGCAGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTACTg                                                                                            >  2:451284/1‑140 (MQ=255)                             ggtgTTCTTCGGCTTTGGTTTTAAAGCCACTATGCCGCCCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTgg                                                                                           <  1:245697/140‑1 (MQ=255)                              gtgtTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGa                                                                                          >  1:521878/1‑140 (MQ=255)                                        gCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGAt                                                                                <  1:115533/140‑1 (MQ=255)                                                   gAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGATGTTGCGCTGTTTGCTGAAACACCCGGATAACGGTATCGa                                                                     >  2:301283/1‑140 (MQ=255)                                                                  accaccGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCTCAGTTTGCAGGATCAGTCGGATAACAGTATAGATGCGTTCCTCGCGcc                                                      >  2:208187/1‑140 (MQ=255)                                                                    caccGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTACTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCgg                                                    <  1:451284/140‑1 (MQ=255)                                                                      ccGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGt                                                  <  1:503973/140‑1 (MQ=255)                                                                         cTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCAc                                               <  1:644676/140‑1 (MQ=255)                                                                           aTCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGt                                             <  1:143857/140‑1 (MQ=255)                                                                           aTCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGt                                             <  1:501250/140‑1 (MQ=255)                                                                                   gCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGAATCGATGAGTTTCTTGCGCCGAGTCACGTCAGTATgg                                     >  1:297183/1‑140 (MQ=255)                                                                                    cAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGt                                    >  1:469765/1‑140 (MQ=255)                                                                                          gCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCgg                              >  2:25789/1‑140 (MQ=255)                                                                                          gCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCgg                              >  2:720884/1‑140 (MQ=255)                                                                                                   cgTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGc                     >  1:75115/1‑140 (MQ=255)                                                                                                    gTGATGTGCAGAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGAGCCGGGTTACGGCAATATGGTGATCGGCACCGACGcc                    >  1:191086/1‑140 (MQ=255)                                                                                                              gAATTTTTACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTa          >  2:464355/1‑140 (MQ=255)                                                                                                                    ttACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGc      >  1:512766/1‑138 (MQ=255)                                                                                                                    ttACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGc      <  2:512766/138‑1 (MQ=255)                                                                                                                    ttACTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCa    >  2:402366/1‑140 (MQ=255)                                                                                                                      aCTTCTTCTGTCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGc  >  2:50576/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                  TGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGC  >  NC_000913/2852527‑2852783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑