Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,872,552	T→C	D90G (GAC→GGC)	ftsB ←	cell division protein FtsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,872,552	0	T	C	100.0%	101.9 / NA	29	D90G (GAC→GGC)	ftsB	cell division protein FtsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

GACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGTCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTT  >  NC_000913/2872415‑2872682                                                                                                                                          |                                                                                                                                   gACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCa                                                                                                                                  >  2:151647/1‑140 (MQ=255)      tCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCAc                                                                                                                             >  1:381657/1‑140 (MQ=255)          tttGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGa                                                                                                                         <  1:151647/140‑1 (MQ=255)           ttGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGAATGTTTTGCGCCGGAGATTGTGCGCGATTCGCCGCGCCAGGCACCAGAc                                                                                                                        >  2:218505/1‑140 (MQ=255)            tGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACg                                                                                                                       <  1:598790/140‑1 (MQ=255)                           aaTCCGACCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGcc                                                                                                        <  2:395244/140‑1 (MQ=255)                                        gAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCTACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATg                                                                                           <  2:459306/140‑1 (MQ=255)                                                                 ggTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCtt                                                                  >  1:534006/2‑140 (MQ=255)                                                                      ttGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAgc                                                             <  2:381657/140‑1 (MQ=255)                                                                        gCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAgcgc                                                           >  2:407424/1‑140 (MQ=255)                                                                           aTGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCACGGCCTGGTCATGCAGGGTTCATTAGGCGCAGGCTCTTCTAGCGcctc                                                        >  2:339378/1‑140 (MQ=255)                                                                             gTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGcctcct                                                      <  1:711258/140‑1 (MQ=255)                                                                                aaTTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGgc                                                   >  1:502234/1‑140 (MQ=255)                                                                                aaTTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGgc                                                   <  1:407424/140‑1 (MQ=255)                                                                                aaTTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCATGGTCATGTTGACTTCTTTACGCGCACGCTGTTCGAGCGCCTCCTGgc                                                   >  2:223566/1‑140 (MQ=255)                                                                                  ttCCCGGGCTGATTTTTCGATTTTTCTGCTCCGCAGAATGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGgccg                                                 <  1:218505/140‑1 (MQ=255)                                                                                    cccGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGgccgcc                                               >  1:447381/1‑140 (MQ=255)                                                                                     ccGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCa                                              <  2:502234/140‑1 (MQ=255)                                                                                        ggCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTGTTGGAGCGCGTCCTGGCCGCCATTg                                           >  1:311270/1‑140 (MQ=255)                                                                                         gCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGgc                                                   <  1:130314/131‑1 (MQ=255)                                                                                         gCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGgc                                                   >  2:130314/1‑131 (MQ=255)                                                                                             aTTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTAATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATc                                      >  2:499993/1‑140 (MQ=255)                                                                                                       tGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCAt                                             <  2:73618/123‑1 (MQ=255)                                                                                                       tGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCAt                                             >  1:73618/1‑123 (MQ=255)                                                                                                       tGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATCGTCAATTTCg                            >  1:39063/1‑140 (MQ=255)                                                                                                              ccccGCAGACTGTGCGCGCTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATCGTCAATTTCGGCaaaaa                     >  2:328667/1‑140 (MQ=255)                                                                                                                         gtgCGCGCTTCGACGAGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGttt          <  1:328667/140‑1 (MQ=255)                                                                                                                           gcgcgcTTCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTcg        >  1:675679/1‑140 (MQ=255)                                                                                                                                 ttCGACGCGCCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCttt  >  1:642199/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   GACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGGTTGCCATGTTAATTCCCGGGCTGATTTATCGATTGTTTTGCCCCGCAGACTGTGCGCGCTTCGACGCGTCAGGCACCAGACGATAAAAAGTTTCGCCCGGCCTGGTCATGCTGAGTTCATTACGCGCACGCTCTTCGAGCGCCTCCTGGCCGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTT  >  NC_000913/2872415‑2872682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑