Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,965,754	A→G	V95A (GTC→GCC)	lgt ←	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,754	0	A	G	100.0%	54.1 / NA	16	V95A (GTC→GCC)	lgt	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/12);  total (4/12)

ACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCC  >  NC_000913/2965618‑2965887                                                                                                                                         |                                                                                                                                      aCCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGgcac                                                                                                                                    <  2:587937/140‑1 (MQ=255) aCCAAGACCAAACGGAATGAGTCGTGCGATAAAATCAGAGACCTGGAACAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGgcac                                                                                                                                    <  2:280510/140‑1 (MQ=255)    aaGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGa                                                                                                                                 <  1:38976/140‑1 (MQ=255)    aaGACCAAACGGAATGAGTGGTCCGATAAAACCAGAGACCTGGAAGAAAGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGa                                                                                                                                 <  2:267239/140‑1 (MQ=255)     aGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGaa                                                                                                                                <  1:680159/140‑1 (MQ=255)                   gAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGAt                                                                                                                  <  1:366683/140‑1 (MQ=255)                                  agacaggaCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCg                                                                                                   <  1:411353/134‑1 (MQ=255)                                            tGGAAGAAGGAACGTCTAGTACAGCGGGCGAAGATAAACATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTa                                                                                         <  1:331892/140‑1 (MQ=255)                                                agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                                                                     <  2:61972/140‑1 (MQ=255)                                                     gAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                                                                                <  1:34669/140‑1 (MQ=255)                                                                                      accacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAg                                               <  2:40425/140‑1 (MQ=255)                                                                                          cGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAg                                               <  1:554101/136‑1 (MQ=255)                                                                                          cGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAg                                               >  2:554101/1‑136 (MQ=255)                                                                                                cGCCAATCAGGCCGCCGCGGAACGACATGCCCCCGCCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCAAGGAAGACGCCGAGGAAGCCCGCa                                     <  2:287510/140‑1 (MQ=255)                                                                                                             gccgTGGAAAGACATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGtttt                        >  1:549800/1‑140 (MQ=255)                                                                                                                         cATGCCGCCGTCCCAGGCACGGAACTGATTCAGCGGACCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCAtttt            <  1:288923/140‑1 (MQ=255)                                                                                                                         cATGCCGCCGTCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCAtttt            >  2:639222/1‑140 (MQ=255)                                                                                                                                   tCCCAGGCACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGcc  >  2:636868/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      ACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCC  >  NC_000913/2965618‑2965887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑