| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,966,716 | T→C | E573E (GAA→GAG) | ptsP ← | phosphoenolpyruvate‑protein phosphotransferase PtsP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,966,716 | 0 | T | C | 95.7% | 158.3 / NA | 47 | E573E (GAA→GAG) | ptsP | phosphoenolpyruvate‑protein phosphotransferase PtsP |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (21/24); total (21/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGA > NC_000913/2966581‑2966845 | gCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCaa > 1:735626/1‑140 (MQ=255) aaCAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCa > 2:36650/1‑140 (MQ=255) agaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAggcgg > 2:559451/1‑140 (MQ=255) agaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAggcgg > 1:513637/1‑140 (MQ=255) gaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATGTCCTCGAGTTCACGACCGGCATGCTCAATCAggcggc > 1:303277/1‑140 (MQ=255) gaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAggcggc > 2:16769/1‑140 (MQ=255) aTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGgcggcg < 2:371324/140‑1 (MQ=255) tGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGt < 2:727809/140‑1 (MQ=255) aaTCGACCCGCTTTGACAGATGAGGTAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCt < 1:338981/140‑1 (MQ=255) aTCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCtt > 1:699378/1‑140 (MQ=255) cGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTtca > 1:550746/1‑140 (MQ=255) aCCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTtcatc > 1:599920/1‑140 (MQ=255) gCTTTGCCAGATGCGGCCGCATAAATACCATTGACCGCAATTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACt < 2:303277/140‑1 (MQ=255) tttGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTc > 2:366933/1‑140 (MQ=255) ccAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCg < 1:53504/140‑1 (MQ=255) ccAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCg < 2:436534/140‑1 (MQ=255) cAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGa < 1:366933/140‑1 (MQ=255) cAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGa < 1:309526/140‑1 (MQ=255) aTAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCAt < 2:735626/140‑1 (MQ=255) tAAATACCATTGACGGCACTTCGAGCATGATCCCGCTACCTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATc < 2:226997/140‑1 (MQ=255) tAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATc < 2:513637/140‑1 (MQ=255) aaTACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCgg < 2:599920/140‑1 (MQ=255) aTACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGc > 2:406353/1‑140 (MQ=255) tACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa < 2:670896/140‑1 (MQ=255) tACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa < 2:699378/140‑1 (MQ=255) aCCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCaa > 1:377655/1‑140 (MQ=255) cATTGACGGCACTTCCAGCATGTTGCCGATCGGAGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa < 2:339854/140‑1 (MQ=255) cATTGACGGCACTTCCAGCATGCTGCCGATACGTGTTTTGGGAATTTCCTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa < 2:314055/140‑1 (MQ=255) aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTGCATCAACGTCATCGGGGCTTGAGACCATCGGCAACAg > 1:210363/1‑140 (MQ=255) tGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTAATCCAGGCTTGTGTCCATCGGCAACAGaa > 1:262651/1‑140 (MQ=255) aCGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAata > 1:92590/1‑140 (MQ=255) ggCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa > 2:92447/1‑130 (MQ=255) ggCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa < 1:92447/130‑1 (MQ=255) ttCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGt > 1:586593/1‑140 (MQ=255) atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGc > 1:696287/1‑140 (MQ=255) atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGc > 2:505294/1‑140 (MQ=255) gCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCgg < 2:35460/140‑1 (MQ=255) gATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCAt < 2:550746/140‑1 (MQ=255) tttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc < 1:505294/140‑1 (MQ=255) tttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc < 1:436610/140‑1 (MQ=255) tttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc < 2:74623/140‑1 (MQ=255) tAACCGATCATCTCCTCGACTTTACGTCCGGTACGTTCAATCAGGCGGTGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCAc < 2:267949/140‑1 (MQ=255) cGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTgg < 1:730176/140‑1 (MQ=255) atcatcTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGAt > 2:615956/1‑140 (MQ=255) tctcCTCGACTTCACCTCCGGCGCGTTCATTCAGGCGACGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCaag < 1:283165/140‑1 (MQ=255) ctcctcGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCaaga < 1:151564/140‑1 (MQ=255) cGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGAtc > 2:499669/1‑140 (MQ=255) cACGTCCGGCACGCTCAATCA‑‑‑GGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGa > 1:29069/1‑140 (MQ=255) | GCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGA > NC_000913/2966581‑2966845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |