Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,850	A→T	I363F (ATC→TTC)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,850	0	A	T	98.7%	249.9 / NA	75	I363F (ATC→TTC)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (45/28);  minor base C (0/1);  total (46/29)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTC  >  NC_000913/3009716‑3009984                                                                                                                                       |                                                                                                                                       tGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGAATTCCGGTGTTCGGc                                                                                                                                   >  1:316570/1‑140 (MQ=255)         gaTCACTTCACCGTTCAAGCACCGGTGAATGCCTACGCAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGccc                                                                                                                           <  1:283954/140‑1 (MQ=255)               ttCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGACCGGCACAGCATTCCGATGTTCGGCTTTGGCCCGGGTaa                                                                                                                     >  2:289484/1‑140 (MQ=255)                 cACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTCTCATGGGCAGTCACGGGATTCCGGTGTTCGGCTTTGGCCCGGGTAAAg                                                                                                                   >  2:203376/1‑140 (MQ=255)                    cGTTAAAGCACTGGTGAGTGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAAc                                                                                                                <  2:264504/140‑1 (MQ=255)                      ttAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCt                                                                                                              >  1:666617/1‑140 (MQ=255)                         aaGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGaa                                                                                                           >  2:701295/1‑140 (MQ=255)                              cTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCGGGTATTCGGCTTTGGCACGGGAAAAGAACCTGAAGCGCa                                                                                                      >  2:322466/1‑140 (MQ=255)                               tGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCAt                                                                                                     >  1:523361/1‑140 (MQ=255)                                 gTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGc                                                                                                   >  1:5610/1‑140 (MQ=255)                                      tGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTa                                                                                              >  1:147900/1‑140 (MQ=255)                                      tGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTa                                                                                              >  1:124401/1‑140 (MQ=255)                                           aCGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGaa                                                                                         >  1:608212/1‑140 (MQ=255)                                           aCGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGaa                                                                                         >  2:22986/1‑140 (MQ=255)                                           aCGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGaa                                                                                         >  2:145254/1‑140 (MQ=255)                                           aCGAAGGGCTGTTTGGCAAAGCACCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGaa                                                                                         >  1:154949/1‑140 (MQ=255)                                              aaGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCTGGGTAAAGAACCTGAAGCGCATGCACCTAACGaaaaa                                                                                      >  1:256258/1‑140 (MQ=255)                                              aaGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGaaaaa                                                                                      >  2:120623/1‑140 (MQ=255)                                                gggCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAAc                                                                                    >  2:66812/1‑140 (MQ=255)                                                  gCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCt                                                                                  >  2:725684/1‑140 (MQ=255)                                                   cTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTg                                                                                 <  1:725684/140‑1 (MQ=255)                                                      tttGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGaa                                                                              >  1:526181/1‑140 (MQ=255)                                                      tttGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGaa                                                                              >  1:151212/1‑140 (MQ=255)                                                           cAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAAtctc                                                                         >  1:458181/1‑140 (MQ=255)                                                           cAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAAtctc                                                                         >  1:722574/1‑140 (MQ=255)                                                             aaGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCAc                                                                       <  2:458181/140‑1 (MQ=255)                                                                cgcCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTg                                                                    <  1:145254/140‑1 (MQ=255)                                                                     gttgttGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGAc                                                               <  2:734141/140‑1 (MQ=255)                                                                               aGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCcgcg                                                     <  2:158603/140‑1 (MQ=255)                                                                                 tGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGAt                                                   >  1:601217/1‑140 (MQ=255)                                                                                   gACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGt                                                 >  2:139761/1‑140 (MQ=255)                                                                                    aCCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTa                                                <  2:507900/140‑1 (MQ=255)                                                                                        tctcAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCt                                            >  2:548935/1‑140 (MQ=255)                                                                                         ctcAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCCCCTTGTGACCTGTGCCGCGATGTACGCTg                                           >  1:319462/1‑140 (MQ=255)                                                                                         ctcAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTg                                           >  1:496700/1‑140 (MQ=255)                                                                                         ctcAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGC‑TTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGc                                          >  2:366657/1‑140 (MQ=255)                                                                                                aaCGGCGTATCTATCATGGGCCGTCACTGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATccc                                    >  1:79922/1‑140 (MQ=255)                                                                                                aaCGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATccc                                    >  2:505788/1‑140 (MQ=255)                                                                                                      gTATCTATCATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAg                              >  2:738467/1‑140 (MQ=255)                                                                                                      gTATCTATCATGGGCAGTCACGTCATTCCCGTGTTCGTCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAg                              <  1:203376/140‑1 (MQ=255)                                                                                                       tatctatcATGGGCCGTCACGGCATTCCGGTGTTCGGTTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgc                             <  1:289484/140‑1 (MQ=255)                                                                                                       tatctatcATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgc                             <  1:738467/140‑1 (MQ=255)                                                                                                       tatctatcATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgc                             <  2:523361/140‑1 (MQ=255)                                                                                                            atcATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgctggct                        <  1:487240/140‑1 (MQ=255)                                                                                                             tcATGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgctggctg                       >  2:386378/1‑140 (MQ=255)                                                                                                              cATGGGCCGTCACGGCATTCCGTTGTTCGGCCTTGCCCCAGGTAAAGACCCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGctggctgg                      <  2:222056/140‑1 (MQ=255)                                                                                                                tGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCGCCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCa                    <  2:608212/140‑1 (MQ=255)                                                                                                                tGGGCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCa                    <  2:5610/140‑1 (MQ=255)                                                                                                                 gggCCGTCACGGCATTCGGGTGCTCGGCTTTGGTCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCaa                   <  2:327725/140‑1 (MQ=255)                                                                                                                 gggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCaa                   <  1:505788/140‑1 (MQ=255)                                                                                                                 gggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCaa                   >  2:542810/1‑140 (MQ=255)                                                                                                                  ggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTAGGCTGCAGTCCCGTAAAGCTGGCTGGCAAc                  >  1:222056/1‑140 (MQ=255)                                                                                                                  ggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGctggctgg                      <  2:696623/136‑1 (MQ=255)                                                                                                                  ggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGctggctgg                      >  1:696623/1‑136 (MQ=255)                                                                                                                  ggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAAc                  <  2:666617/140‑1 (MQ=255)                                                                                                                  ggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAAc                  <  2:496700/140‑1 (MQ=255)                                                                                                                  ggCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGCGACCTGTGGCGCGAAGTACGCTGCAATCCCGTTAAGCTGCCTGGCAAc                  >  2:338995/1‑140 (MQ=255)                                                                                                                   gCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTTACCTGTGCCGCGCTGTCCGCTGCAACCCCGGTAAGCTGGCTGGCAACa                 >  2:272788/1‑140 (MQ=255)                                                                                                                   gCCGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCGGCGATGTGCGCTGCAAGCCCGTTAAGCTGGCTGGCAACa                 >  2:211575/1‑140 (MQ=255)                                                                                                                    ccGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACag                <  1:542810/140‑1 (MQ=255)                                                                                                                     cGTCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGAGCTGTGCCGGGATATACACTGCAATCCCGTTAAGCTGGCTGGCAACaga               >  2:235139/1‑140 (MQ=255)                                                                                                                       tCACGGCATTCCGGTGTTCGGCTTTGGCCCGGTTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGt             >  2:503073/1‑140 (MQ=255)                                                                                                                       tCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATGCCGTTAAGCTGGCTGGCAACAGAGt             >  1:327725/1‑140 (MQ=255)                                                                                                                       tCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACag                >  1:551681/1‑137 (MQ=255)                                                                                                                       tCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACag                <  2:551681/137‑1 (MQ=255)                                                                                                                       tCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGt             >  1:695977/1‑140 (MQ=255)                                                                                                                       tCACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGt             >  2:695143/1‑140 (MQ=255)                                                                                                                        cACGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTTTGCCGCGAGGTACGCTGCAATCCCGTTAAGTTGGCTGACAACAGAGTa            >  2:304231/1‑140 (MQ=255)                                                                                                                          cGGCATTCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAAt          <  2:601217/140‑1 (MQ=255)                                                                                                                               ttCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTActc     <  2:691249/140‑1 (MQ=255)                                                                                                                               ttCCGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTActc     <  1:503073/140‑1 (MQ=255)                                                                                                                                tCCGGTGTTCGGCTTTCACCCGAGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTActct    <  2:316570/140‑1 (MQ=255)                                                                                                                                 ccGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCtt   >  1:737141/1‑140 (MQ=255)                                                                                                                                  cGGTGTTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTc  <  1:66812/140‑1 (MQ=255)                                                                                                                                  cGGTGTTCGGCTTTGACCCGGGTAAAGAATCTGAAGCCCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTc  <  1:235139/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTC  >  NC_000913/3009716‑3009984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑