| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,051,344 | T→C | D325G (GAC→GGC) | ubiI ← | 2‑octaprenylphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,051,344 | 0 | T | C | 95.0% | 131.7 / ‑0.1 | 40 | coding (974/1203 nt) | ubiI | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (18/20); minor base . (1/1); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTG > NC_000913/3051215‑3051474 | cGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTg > 1:632342/1‑140 (MQ=255) gTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGc < 1:148643/140‑1 (MQ=255) tCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGt > 2:173644/1‑140 (MQ=255) tCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGt > 1:420610/1‑140 (MQ=255) gCCAGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGttt > 2:664213/1‑140 (MQ=255) cGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCa < 1:137177/140‑1 (MQ=255) tGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCg < 1:392188/140‑1 (MQ=255) gAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAg < 1:644730/140‑1 (MQ=255) cAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTg < 1:664213/140‑1 (MQ=255) tCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTgcagc < 2:632342/140‑1 (MQ=255) gcgGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCAt > 2:484/1‑140 (MQ=255) aTCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATa < 1:394765/140‑1 (MQ=255) cTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGc > 2:734367/1‑140 (MQ=255) ccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAcc > 2:12384/1‑140 (MQ=255) agcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCAGCACCACCCATAAAGCCGAGATTTAcccc > 1:312686/1‑140 (MQ=255) cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCCCGGCAGCATCCATAACGCCGAGATTTACCCCCt > 2:323416/1‑140 (MQ=255) cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATG‑CTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTg > 1:489402/1‑140 (MQ=255) ccgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGcc < 2:30221/140‑1 (MQ=255) gcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCCTAAAGCCGGGATTTACCCCCTGCCCCGCCAg > 2:308545/1‑140 (MQ=255) gcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAg < 2:483719/140‑1 (MQ=255) cACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGc > 1:509685/1‑140 (MQ=255) aCTGTGCTTGCGGCTACGCTCATAGGGACACAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCg < 1:323416/140‑1 (MQ=255) tgtgCTTGCGGCTACGCACATATCGACGCAGCTTACTGTTCTGCCCGAGGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCggg < 2:312686/140‑1 (MQ=255) gtgCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGt < 2:99226/140‑1 (MQ=255) ttGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAAt < 1:173644/140‑1 (MQ=255) aGCGACGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCg > 1:416935/1‑140 (MQ=255) aCGCAGATAAATGTACTGCCCGATG‑CTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac < 2:489402/140‑1 (MQ=255) cGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac > 2:148754/1‑140 (MQ=255) cGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac < 1:108414/140‑1 (MQ=255) cGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac < 2:416935/140‑1 (MQ=255) cGCAGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac < 1:547590/140‑1 (MQ=255) cGCAGATAAATGTACTGCCCGACGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac < 1:236189/140‑1 (MQ=255) aGATAAATGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAg > 1:382022/1‑140 (MQ=255) aaTGTACTGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCa < 1:12384/140‑1 (MQ=255) tGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGTCAGCGGGTTAATGGTAGGTGCGGCGCCGCCTACCAGCGCCAGACGGTg > 2:241939/1‑140 (MQ=255) tGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg > 2:32046/1‑140 (MQ=255) tGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg < 2:420610/140‑1 (MQ=255) tGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg > 1:17658/1‑140 (MQ=255) tGCCCGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg < 2:700804/140‑1 (MQ=255) ggCCCGACGCCTTCCCCCTGACGTTCCAGCCGTTTCAGTTCGAGAATCAGCCCTGCAGCATCCATAAAGCGGAGATTTCCCCCCTCCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg < 2:382022/139‑1 (MQ=255) cccGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac > 2:446538/1‑123 (MQ=255) cccGATGCCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccac < 1:446538/123‑1 (MQ=255) | CGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTG > NC_000913/3051215‑3051474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |