Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,593	A→G	N71N (AAT→AAC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,593	0	A	G	100.0%	76.0 / NA	23	N71N (AAT→AAC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

GCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGCCAGAACGCCGCAGCGTCCAGCCCTGTTCCC  >  NC_000913/3123460‑3123728                                                                                                                                      |                                                                                                                                        gCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTtgctg                                                                                                                                   <  1:650295/140‑1 (MQ=255)  cGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTtgctgc                                                                                                                                  <  1:573393/140‑1 (MQ=255)       ccGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGc                                                                                                                             <  2:181916/140‑1 (MQ=255)        cGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGcc                                                                                                                            >  1:500169/1‑140 (MQ=255)              gctggTGATTTCGCTGTCAATGACCGTGGATTCCACCATCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACtcat                                                                                                                      <  1:329723/140‑1 (MQ=255)                  gTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAg                                                                                                                  <  2:651394/140‑1 (MQ=255)                                            ttCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCTCTCATCAAGCGCGGCCTGCATGCGATCGCGTTCTg                                                                                        >  2:334416/1‑140 (MQ=255)                                                       cgcgCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAACGCGATCGCGTTCTGCCAGCAACTGa                                                                             <  2:500169/140‑1 (MQ=255)                                                               ggTTGCGGCACCAGGTAGCCCATTTCACGCACGAATGATCTATAGGCCACTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTc                                                                     <  2:228249/140‑1 (MQ=255)                                                                     ggCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGc                                                               <  1:583912/140‑1 (MQ=255)                                                                     ggCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGc                                                               >  2:466867/1‑140 (MQ=255)                                                                     agCACCAGGTAGCCCAGTTCACGAAGGAAAGATTTATAGGCCGCTTTATCTTTTCCCGGCCCCGGGTTGCTGCGATGCCAATCATCAAGCGCTGCCTGAATGCGGTCGCGTTCTGCCAGCAACTGACGACTTTCTGGTGc                                                               <  2:17172/139‑1 (MQ=255)                                                                          cAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGAt                                                          <  1:75261/140‑1 (MQ=255)                                                                                 cccAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAAc                                                   >  1:71886/1‑140 (MQ=255)                                                                                  ccAGTTCACGCAGGATAGATTTCTATGCCACTTTATCCTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACg                                                  <  1:223003/140‑1 (MQ=255)                                                                                    aGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGAt                                                <  1:647212/140‑1 (MQ=255)                                                                                    aGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGAt                                                >  2:670007/1‑140 (MQ=255)                                                                                      ttCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGAtct                                              >  1:701623/1‑140 (MQ=255)                                                                                                     tttATAGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGcc                               >  1:403107/1‑140 (MQ=255)                                                                                                         taGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGCCAGaa                           <  1:672053/140‑1 (MQ=255)                                                                                                         taGGCCGCTTTATCTTTTACCGGCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGCCAGaa                           >  2:672053/1‑140 (MQ=255)                                                                                                                               gCCCCGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGCCAGAACGCCGCAGCGTCCAGCCCTGtt     >  2:72580/1‑140 (MQ=255)                                                                                                                                ccccGGGTTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGCCAGAACGCCGCAGCGTCCAGCCCTGTTc    <  1:670007/140‑1 (MQ=255)                                                                                                                                  ccGGGTTGCTGCGATCCCACTCATCCAGCCCTGCCTGTATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGCCAGAACGCCGCAGCGTCCAGCCCTGTTccc  <  1:334416/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        GCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGATCTCATCAAAATTGCGCCAGAACGCCGCAGCGTCCAGCCCTGTTCCC  >  NC_000913/3123460‑3123728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑