Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,158,920	A→G	intergenic (+36/+7)	yqhH → / ← ygiQ	lipoprotein YqhH/radical SAM superfamily protein YgiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,158,920	0	A	G	100.0%	79.0 / NA	28	intergenic (+36/+7)	yqhH/ygiQ	lipoprotein YqhH/radical SAM superfamily protein YgiQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/11);  total (17/11)

TATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCATCGGCGTATGTTTCGTCAAC  >  NC_000913/3158789‑3159056                                                                                                                                    |                                                                                                                                         tATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTAtttttt                                                                                                                                  <  1:463540/140‑1 (MQ=255)        ccAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTcc                                                                                                                           >  2:676308/1‑140 (MQ=255)        ccAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTcc                                                                                                                           >  2:131322/1‑140 (MQ=255)             tCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTGGCGAGTCTGTTGCCGGGGGTAATTTTTAACGTCCAAccg                                                                                                                      >  2:252539/1‑140 (MQ=255)                       aCAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAgg                                                                                                            >  2:733506/1‑140 (MQ=255)                                aTACGCGTTTTGATGCTCAGGATTTTTTTGATTGCCGGCGCTGCTTGCGTATGTACGCAGAATAATAAAAAAACCCCCGGTAGCGTGTCAGTTGCGGGGGGTTTTTTTTAACGTCCAACCGCCGCTTTAGGCCGTttctt                                                                                                   <  2:623060/140‑1 (MQ=255)                                aTACGCGTCTTGATGCTCAGGACTTTTTTGTTTGCCTGCGCTGCTCGCGTATGTACGCAGAATGAAAAAAAAACCCCCGGTAGCGTGTCAGTTGCGGGGGGTTTTTTTTAACGTCCAACCGCCGCTTTAGGCCGTttctt                                                                                                   <  2:497302/140‑1 (MQ=255)                                aTACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTttctt                                                                                                   >  1:147065/1‑140 (MQ=255)                                    gcgTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCa                                                                                               <  2:385012/140‑1 (MQ=255)                                              gCTCAGGACTATTTTCATTGCCTGCCCTGCTTGCGTAGGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCa                                                                                     <  1:252539/140‑1 (MQ=255)                                                 cAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTGAACGTCCAGCCGCCGCTTTAGGCCGGTTCTTCGCACCAGCATTCACCg                                                                                  >  2:226817/1‑140 (MQ=255)                                                  aGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCgg                                                                                 >  2:127436/1‑140 (MQ=255)                                                   ggACTATTTTGATTGCCTGCGCTGTTTGCGTATGTACGCAGAATAAAAAAAAAACCCCCGGTAGCGTGTCAGTTGCGGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGa                                                                                <  1:148501/140‑1 (MQ=255)                                                       tATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACgag                                                                            >  1:433278/1‑140 (MQ=255)                                                       tATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCCTTAGGCCGTTTATTAGCACCAGCATTCACCGGACgag                                                                            >  1:336626/1‑140 (MQ=255)                                                             gtttcccTGCGCTGCTTGCGTATGTACGAAAAATAAAAAAAAACCCCCGGGTAGCGGTCCAGTTGCCGGGGGTTTTTTTTAACGTCAAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCg                                                                      <  2:147065/135‑1 (MQ=255)                                                                    tGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAgacga                                                               >  2:592775/1‑140 (MQ=255)                                                                                     tACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAg                                              >  2:146776/1‑140 (MQ=255)                                                                                            aaTGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGt                                       >  2:373314/1‑140 (MQ=255)                                                                                                    aaaaaCCCCCGGTGGCGTGTCATTTGCCGGGGGTTTTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCt                               <  1:733506/140‑1 (MQ=255)                                                                                                        aTCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGGTTGCGTAGACGAAGCTCTTTTTGCCATAGCAGGCGTCTGACGCTGGGt                           >  2:323771/1‑140 (MQ=255)                                                                                                        aTCCCCGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGt                           <  1:131322/140‑1 (MQ=255)                                                                                                          ccccGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCg                         >  2:635673/1‑140 (MQ=255)                                                                                                             cGGTAGCGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCa                      <  1:127436/140‑1 (MQ=255)                                                                                                                   cGTGTCAGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCATCGGCg                >  1:431944/1‑140 (MQ=255)                                                                                                                         aGTTGCCGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCATCGGCGTATGtt          >  1:14215/1‑140 (MQ=255)                                                                                                                               cGGGGGTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCATCGGCGTATGTTTCGTCa    >  1:605398/1‑140 (MQ=255)                                                                                                                                ggggGTTTTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCATCGGCGTATGTTTCGTCaa   <  2:14215/140‑1 (MQ=255)                                                                                                                                 ggggTATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCATCGGCGTATGTTTCGTCAAc  <  1:592775/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         TATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCGTAGACGACGCTTTTTTTGCCGTAGCAGGCGTCTGACGCTGGGTCGCCATCGGCGTATGTTTCGTCAAC  >  NC_000913/3158789‑3159056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑