| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 477,430 | T→C | E396E (GAA→GAG) | pdeB ← | c‑di‑GMP phosphodiesterase PdeB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 477,430 | 0 | T | C | 98.3% | 208.6 / NA | 60 | E396E (GAA→GAG) | pdeB | c‑di‑GMP phosphodiesterase PdeB |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (23/35); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGTCGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATG > NC_000913/477295‑477561 | aaTGTCGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAg > 2:52173/1‑140 (MQ=255) tGTCGACATCCAGATCCCGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTg > 1:138190/1‑140 (MQ=255) gTCGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGa > 2:145519/1‑140 (MQ=255) aCATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAAATATCGGGGAGCTAGTTGTCGGATCGGCAAAATCGCGCTCAGTGAGTTc > 2:192998/1‑140 (MQ=255) tCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAg < 1:541493/140‑1 (MQ=255) tCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTACCGGGGCGCAGGTCTCCGGATCGGCATACTCCTGCTCAGTGAGTTCAAg > 2:353014/1‑140 (MQ=255) cAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAgcg < 2:293522/140‑1 (MQ=255) gATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGcgcg > 2:132786/1‑140 (MQ=255) aTCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGa > 1:101902/1‑140 (MQ=255) tCCTGTAAATAACTTGAACTTGCATGCCCAGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 1:340455/140‑1 (MQ=255) tCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 2:680058/140‑1 (MQ=255) tCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 1:397433/140‑1 (MQ=255) tCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 1:428461/140‑1 (MQ=255) tCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 1:52173/140‑1 (MQ=255) tCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 1:523972/140‑1 (MQ=255) tCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 1:158040/140‑1 (MQ=255) tCCCGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 2:138190/140‑1 (MQ=255) tGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAtctg > 1:588459/1‑140 (MQ=255) tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAtctgtc > 1:426142/1‑140 (MQ=255) tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt < 2:533839/135‑1 (MQ=255) tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGAt > 1:533839/1‑135 (MQ=255) tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTAGCGGTCTGTGAGTGCAAGCGCGAtctgtc > 1:211990/1‑140 (MQ=255) aCTTAAACTTGCATAGCCCGTAGCACAATGATCAAGACAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGAt < 1:345626/140‑1 (MQ=255) aaaCTTGACTACCCCGTCCCAATATCATCAGTAGACATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAAc < 2:355883/140‑1 (MQ=255) aaaCTTGAATACCCCGTCCCAAAATCATCAATATACATTTCATGGCCCACCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAAc < 2:195085/140‑1 (MQ=255) aaaCTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAAc < 2:41327/140‑1 (MQ=255) aCTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCt < 2:577406/140‑1 (MQ=255) tGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt < 2:144687/140‑1 (MQ=255) cccGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt < 1:689491/133‑1 (MQ=255) cccGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt > 2:689491/1‑133 (MQ=255) cccGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgatt > 2:128487/1‑140 (MQ=255) cccGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgatt > 1:185983/1‑140 (MQ=255) ccGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGGTCAAGCGGGATCTGTCAGGGAGTAACCTGATAGtgattg > 2:296806/1‑140 (MQ=255) cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCGAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtg < 1:33766/135‑1 (MQ=255) cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCGAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtg > 2:33766/1‑135 (MQ=255) aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCa > 2:180280/1‑140 (MQ=255) aaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCa < 1:145519/140‑1 (MQ=255) aTGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGAttt > 1:52789/1‑140 (MQ=255) tGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGAtttt > 2:648499/1‑140 (MQ=255) cccGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTc > 1:514089/1‑140 (MQ=255) ccGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTcc > 2:619353/1‑140 (MQ=255) ccGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTcc < 2:170676/140‑1 (MQ=255) cGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGCTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCg > 2:550623/1‑140 (MQ=255) ccTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg < 1:619353/140‑1 (MQ=255) ccTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg < 2:426142/140‑1 (MQ=255) cccGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTg < 2:514089/140‑1 (MQ=255) cGGTAGCGAGAAATTCTCGGGGCGCTGGTCTTCGGATTGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAg < 1:192998/140‑1 (MQ=255) aGCGAGAAATTATCGGGGCGCTAGTTTTCGGTTCGCCAATCTCGCGCTAAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACg < 2:293092/140‑1 (MQ=255) gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg < 1:132786/140‑1 (MQ=255) gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg > 1:709541/1‑140 (MQ=255) gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg > 1:151577/1‑140 (MQ=255) cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg < 1:180280/140‑1 (MQ=255) agaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg < 2:624832/138‑1 (MQ=255) agaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg > 1:624832/1‑138 (MQ=255) cTGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGa > 1:582863/1‑140 (MQ=255) tGGTTTTCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGaa < 2:582863/140‑1 (MQ=255) ttCGGATCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATAtg < 1:116854/140‑1 (MQ=255) aTCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATgctgct < 2:185983/140‑1 (MQ=255) tCGGCAAACTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGctgctg < 1:550623/140‑1 (MQ=255) aaCTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATg < 2:151577/140‑1 (MQ=255) aaCTCGCGCTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATg < 2:709541/140‑1 (MQ=255) | AATGTCGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATG > NC_000913/477295‑477561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |