Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,198,044	A→G	V533A (GTG→GCG)	glnE ←	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,198,044	0	A	G	100.0%	46.3 / NA	14	V533A (GTG→GCG)	glnE	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/6);  total (8/6)

TCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTT  >  NC_000913/3197909‑3198165                                                                                                                                        |                                                                                                                          tCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCGCCAg                                                                                                                       <  2:565702/140‑1 (MQ=255)         cAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGAAATTCACTGAGCAATTCTAAATAGGTGGGGCGGGTAACAATCCCCGCCAGCAAGGCgg                                                                                                               <  1:104794/140‑1 (MQ=255)         aaataatGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATAAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATGGGTGGTGCGGGTAACAATCCCCGCCAGCAAGGCgg                                                                                                               <  2:549720/139‑1 (MQ=255)            taatGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCGCCAGCAAGGCGGTaa                                                                                                            >  1:185325/1‑140 (MQ=255)              atGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCGCCAGCAAGGCGGTAATg                                                                                                          >  1:716104/1‑140 (MQ=255)                              ctggctggCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACgg                                                                                          >  1:569005/1‑140 (MQ=255)                                              ggggACGCGGCACAAAGAGAAATAAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGTGGGGGTAACAACCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACgcgc                                                                          <  2:185325/137‑1 (MQ=255)                                                                                   ccgcGGGGAATTCACTGAGCAATTCAAAATAGGTGGTGCGGGTAACAATCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCg                                     <  2:68913/140‑1 (MQ=255)                                                                                                    acaaaTTCTAAATAGGTGGTGCGGGTAACAACCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTcg                    <  2:716104/137‑1 (MQ=255)                                                                                                              aaTAGGTGGTGCGGGTAACAATCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATg          >  1:121132/1‑140 (MQ=255)                                                                                                                   gtggtgCGGGTAACAATCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTgcg     >  1:586754/1‑140 (MQ=255)                                                                                                                   gtggtgCGGGTAACAATCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTgcg     >  1:626738/1‑140 (MQ=255)                                                                                                                   gtggtgCGGGTAACAATCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTgcg     >  2:518391/1‑140 (MQ=255)                                                                                                                      gtgCGGGTAACAATCCCCGCCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACTTGACGTACTCGCGACCCGATGGTGCGCtt  >  1:309377/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                          TCATCCAGCAATAATGGATAACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGAATTCACTGAGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTT  >  NC_000913/3197909‑3198165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑