Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,229,583	T→C	G232G (GGT→GGC)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,229,583	0	T	C	100.0%	121.1 / NA	34	G232G (GGT→GGC)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/21);  total (13/21)

AGCGCAAAATCAGCGCCACCCGTGATGGCCTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGTAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTAT  >  NC_000913/3229447‑3229718                                                                                                                                         |                                                                                                                                        aGCGCAAAATCAGCGCCACCCGTGATGGCCTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAAt                                                                                                                                      <  2:384658/140‑1 (MQ=255) aGCGCAAAATCAGCGCCACCCGTGATGGCCTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAAt                                                                                                                                      <  1:734618/140‑1 (MQ=255)     cAAAATCAGCGCCACCCGTGATGGCCTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAATTCCCTGCGGGTTCAGACTGAAATCAACGGCAATCGCt                                                                                                                                  >  1:199459/1‑140 (MQ=255)      aaaaTCAGCGCCACCCGTGATGGCCTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCtt                                                                                                                                 <  1:396832/140‑1 (MQ=255)             gcgcCACCCGTGATGGCCTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGt                                                                                                                          <  1:638775/140‑1 (MQ=255)                 cagccGTGATGGCCTGATAGTCCCCTTTGGCAAAGCGCGCGCCACCTGCGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAgg                                                                                                                      <  2:204437/137‑1 (MQ=255)                             ccTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACg                                                                                                          >  2:636958/1‑140 (MQ=255)                              cTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAACCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACgg                                                                                                         >  1:429003/1‑140 (MQ=255)                              cTGAAAGTCACCTTTAGCAAAGAGCTCGCCACCTGGGGTCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCGGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACgg                                                                                                         <  1:345316/140‑1 (MQ=255)                               tGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAGCGGCAATAGCTATAGCAGTAAGGCGCATATCAAcctg                                                                                                        >  1:204437/1‑137 (MQ=255)                                     tCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGAcc                                                                                                  <  1:404822/140‑1 (MQ=255)                                     tCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCAGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGAcc                                                                                                  <  1:649283/140‑1 (MQ=255)                                      cACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCa                                                                                                 <  1:624659/140‑1 (MQ=255)                                          tttGGCAAAGTGCGCGCTACCTCGGATCTGCCGACTTCCGGCGAATCGCAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGct                                                                                             <  2:199459/140‑1 (MQ=255)                                                              cTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATc                                                                         >  1:376308/1‑140 (MQ=255)                                                                     ctgctgACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCATTAATGCGCATATCACCGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGAc                                                                  <  2:729783/140‑1 (MQ=255)                                                                     ctgctgACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGAc                                                                  <  2:605962/140‑1 (MQ=255)                                                                           aCCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCGCGCTCTACACCACCGATTCCCGTCTGCTGACCGCTCa                                                            >  1:341981/1‑140 (MQ=255)                                                                            ccTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAg                                                           <  1:665617/140‑1 (MQ=255)                                                                              tCCGGCGACTCGGAATCCCAGGAGCATAAAGCCCTGCCGGTGCAGACTAAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGa                                                         <  2:341981/140‑1 (MQ=255)                                                                               ccGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGaa                                                        >  1:376395/1‑140 (MQ=255)                                                                                      aTCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCa                                                 <  2:376395/140‑1 (MQ=255)                                                                                       tCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCaa                                                >  1:370055/1‑140 (MQ=255)                                                                                                 acaggCATAAATCCCGGCCGGTTCACACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATg                                      <  1:275991/136‑1 (MQ=255)                                                                                                    tGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAg                                   <  2:370055/140‑1 (MQ=255)                                                                                                      cATAAATCCCTGCCGGTGCAGACTGAAACCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGAt                                 <  2:429003/140‑1 (MQ=255)                                                                                                          aaTCCCTGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCcgc                             <  2:109628/140‑1 (MQ=255)                                                                                                                tGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATAtt                       >  1:676012/1‑140 (MQ=255)                                                                                                                tGCCGGTGCAGACTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCGCAGGAAGGTAGCAAAGTGCAAATGCAGATCCGCGactgt                       >  2:275991/1‑136 (MQ=255)                                                                                                                           aCTGAAATCAACGGCAACCGCTCTACCAGTACGGCCCATCTCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTcc            <  2:193232/140‑1 (MQ=255)                                                                                                                            cTGAAATCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCGGATCCGCGATATTCTGGCGCGTCCg           <  1:636958/140‑1 (MQ=255)                                                                                                                                aaTCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCTGGCGt       >  1:180982/1‑140 (MQ=255)                                                                                                                                aaTCAACGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGt       >  1:65705/1‑140 (MQ=255)                                                                                                                                     aCGGCAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCGGACGCCTCAGGAAGTAAGCAAAGACCAAATGCAGATCCCGGATATTCTGGCGCGTCCGCCGtttttt  >  2:44554/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCGCAAAATCAGCGCCACCCGTGATGGCCTGAAAGTCACCTTTGGCAAAGTGCGCGCCACCTGGGATCTGCTGACCTCCGGCGAATCGGAATATCAGGTGCATAAATCCCTGCCGGTGCAGACTGAAATCAACGGTAATCGCTTTACCAGTAAGGCGCATATCAACGGTTCGACCACGCTCTACACCACCTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTAT  >  NC_000913/3229447‑3229718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑