Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,316	T→C	A115A (GCA→GCG)	tdcD ←	propionate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,316	0	T	C	100.0%	165.7 / NA	48	A115A (GCA→GCG)	tdcD	propionate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/31);  total (17/31)

GGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTC  >  NC_000913/3263182‑3263439                                                                                                                                       |                                                                                                                            ggCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTACGCCGATTGAATACCACTTAAATTGACGTAGTTATGCAGGGGCGCCAg                                                                                                                        >  2:196546/1‑140 (MQ=255)      gCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTATTTATAGAGGGGCGCCAGTGgag                                                                                                                   >  2:353971/1‑140 (MQ=255)      gCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGgag                                                                                                                   >  1:393287/1‑140 (MQ=255)          tataAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAAc                                                                                                               <  2:104612/140‑1 (MQ=255)          tataAATAAGCTTCCGGAGCCATCGTCTGATGGAAACGGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAAc                                                                                                               <  1:263577/140‑1 (MQ=255)             aaataaGCTTCCGGATCCATCGTCTTGTGCAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAAcgcg                                                                                                            <  1:353971/140‑1 (MQ=255)             aaataaGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAAcgcg                                                                                                            <  1:642459/140‑1 (MQ=255)             aaataaGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAAcgcg                                                                                                            <  2:550552/140‑1 (MQ=255)             aaataaGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAAcgcg                                                                                                            >  1:515044/1‑140 (MQ=255)             aaataaGCTTCCGGAGCCATCGTCTGGTGCAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAAcgcg                                                                                                            <  1:333925/140‑1 (MQ=255)                  aGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGa                                                                                                       <  2:433407/140‑1 (MQ=255)                  aGCTTCCGGAGCCATCGGCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGa                                                                                                       <  2:311139/140‑1 (MQ=255)                      tCCGGAGCCATCGTCTGGTGGACACCGGTATCAAATATCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGAtatt                                                                                                   <  1:223990/140‑1 (MQ=255)                       ccGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAGTTGGCGTATTTATGCATGGGCGCCACTGGAGAAACGCGACGGAtatta                                                                                                  >  2:298219/1‑140 (MQ=255)                        cGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCGACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGAtattat                                                                                                 >  1:154098/1‑140 (MQ=255)                          gAGCCATCGTCTGGTGGAAACTGGTATCACATACCGCGACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCa                                                                                               <  1:360833/140‑1 (MQ=255)                               aTCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGAc                                                                                          <  2:535806/140‑1 (MQ=255)                                tCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACt                                                                                         <  1:603616/140‑1 (MQ=255)                                  gTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTc                                                                                       <  1:2894/140‑1 (MQ=255)                                   tCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCa                                                                                      <  2:10566/140‑1 (MQ=255)                                          gAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGtaa                                                                               <  1:569839/140‑1 (MQ=255)                                               tGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCa                                                                                      >  2:725887/1‑128 (MQ=255)                                               tGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAAGTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCa                                                                                      <  1:725887/128‑1 (MQ=255)                                                  tATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCg                                                                       >  1:397803/1‑140 (MQ=255)                                                    tCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGa                                                                     <  2:397803/140‑1 (MQ=255)                                                    tCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGa                                                                     <  1:174705/140‑1 (MQ=255)                                                       aaTACCGCCACCTGAATTACGCCCGTAAATAATTGCTCCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTc                                                                  <  1:296990/140‑1 (MQ=255)                                                           ccGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTa                                                              >  1:384766/1‑140 (MQ=255)                                                              ccACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTaaaa                                                           >  2:632597/1‑140 (MQ=255)                                                                 ccTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGCATATTATCCATGACATCATCGGTAATAATGTCGGACTCGGTAAAAATa                                                        >  2:311968/1‑140 (MQ=255)                                                                         aCGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTgccgcc                                                >  2:16878/1‑140 (MQ=255)                                                                           gCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGt                                              <  2:85352/140‑1 (MQ=255)                                                                              cGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAg                                           <  1:686797/140‑1 (MQ=255)                                                                              cGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAg                                           <  1:64501/140‑1 (MQ=255)                                                                                     aatTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCg                                    <  1:632597/140‑1 (MQ=255)                                                                                     aatTGCTGCGCCGATCCAATACCACATAAGTTGGCGTCATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCg                                    <  1:196546/140‑1 (MQ=255)                                                                                       ttGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGt                                  <  1:16878/140‑1 (MQ=255)                                                                                         gctgcGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTgg                                <  2:384766/140‑1 (MQ=255)                                                                                          ctgcGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGc                               <  2:531606/140‑1 (MQ=255)                                                                                            gcgcCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATCATGGCGGACTCGGTAAAAATAATGCGGCCGCGAGCCATGCGGTGGCCa                             >  2:296927/1‑140 (MQ=255)                                                                                                  aTTCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAg                       <  2:515044/140‑1 (MQ=255)                                                                                                   ttCAATACCACTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAgg                      <  2:465355/140‑1 (MQ=255)                                                                                                            aCTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCcaca                 <  1:100920/136‑1 (MQ=255)                                                                                                            aCTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCcaca                 >  2:100920/1‑136 (MQ=255)                                                                                                             cTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCTGCCGTGAGCGATGCGGGGGCCGATTAAGGCCACACTGTc            >  2:188625/1‑140 (MQ=255)                                                                                                             cTTAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTc            >  1:374866/1‑140 (MQ=255)                                                                                                              ttAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCcaca                 >  2:636697/1‑134 (MQ=255)                                                                                                              ttAAATTGGCGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCcaca                 <  1:636697/134‑1 (MQ=255)                                                                                                                       cGTAATTATGCAGGGGCGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCCCCGTGAGCAATGCGGTAGCCAATTATGGCCTCACTGTCATTTAAATTc  >  2:227777/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                            GGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTC  >  NC_000913/3263182‑3263439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑