Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,347,536	T→C	V141A (GTT→GCT)	rapZ →	RNase adaptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,347,536	0	T	C	100.0%	123.3 / NA	37	V141A (GTT→GCT)	rapZ	RNase adaptor protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/14);  total (23/14)

AGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGTGCGCTTCTT  >  NC_000913/3347403‑3347671                                                                                                                                      |                                                                                                                                        aGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGa                                                                                                                                   <  2:573920/140‑1 (MQ=255)        cgcgCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAgctggct                                                                                                                            >  2:484533/1‑140 (MQ=255)          cgcCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGAAAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGa                                                                                                                          >  2:32420/1‑140 (MQ=255)           gcCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGaa                                                                                                                         >  2:724284/1‑140 (MQ=255)            ccGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGaaa                                                                                                                        <  1:724284/140‑1 (MQ=255)               aCTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAg                                                                                                                                  <  2:518600/127‑1 (MQ=255)               aCTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAg                                                                                                                                  >  1:518600/1‑127 (MQ=255)                 tGCATCCGCTTTCCACCAAAAATCTTTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAAtgctg                                                                                                                   <  1:206102/140‑1 (MQ=255)                    aTCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGt                                                                                                                <  1:484533/140‑1 (MQ=255)                          tttCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGt                                                                                                          <  1:381736/140‑1 (MQ=255)                           ttCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTTCGCTCACGAGCTCGCTGAAATGCTGCGTACCCGTc                                                                                                         >  2:286916/1‑140 (MQ=255)                                      aCCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTaaa                                                                                              >  2:518056/1‑140 (MQ=255)                                           tCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGa                                                                                         >  1:682551/1‑140 (MQ=255)                                           tCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCAGGCTGAAATGCTGCGTACCCGATTGCTGGGTAAACGTGa                                                                                         >  2:344742/1‑140 (MQ=255)                                            cGCTGGAAAGTGCTACCGACAACGACAGCTATTTGCTTGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTaaa                                                                                        <  1:186899/140‑1 (MQ=255)                                                           tCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATg                                                                         <  2:104944/140‑1 (MQ=255)                                                                aaagaaagCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCtt                                                                    >  1:69957/1‑140 (MQ=255)                                                                        cGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCtt                                                            >  2:412716/1‑140 (MQ=255)                                                                         gATTTGCTGGAGCCTATGCGTTAGCTAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCttt                                                           <  1:344742/140‑1 (MQ=255)                                                                                     ccTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAaca                                               >  1:465681/1‑140 (MQ=255)                                                                                      ctctGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAacac                                              <  2:147822/140‑1 (MQ=255)                                                                                       tctGCGTTCGCGAGCCGATCTGTTTATCGGTACCTCAAAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACg                                             <  1:286916/140‑1 (MQ=255)                                                                                         tGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGt                                           >  2:362795/1‑140 (MQ=255)                                                                                         tGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGt                                           >  2:52303/1‑140 (MQ=255)                                                                                               cgcgAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCaaa                                                 >  2:228727/1‑128 (MQ=255)                                                                                               cgcgAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCaaa                                                 <  1:228727/128‑1 (MQ=255)                                                                                                       gATCTGATTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTAAGTCTTTGGGCCTCTAACACGTTATCCCTATCGATGc                             >  1:247597/1‑140 (MQ=255)                                                                                                             aTTGTCGATACCTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTa                       <  2:682551/140‑1 (MQ=255)                                                                                                                       ccTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGAc             >  1:469468/1‑140 (MQ=255)                                                                                                                       ccTCAGAAATGTCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTAAAAAACGCTATCCCTAACGATTGAGATTACCTCTTTGAc             >  2:361156/1‑140 (MQ=255)                                                                                                                                  tCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGt           <  2:163376/131‑1 (MQ=255)                                                                                                                                  tCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGt           >  1:163376/1‑131 (MQ=255)                                                                                                                                  tCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGTGCGcttctt  >  2:508892/1‑140 (MQ=255)                                                                                                                                  tCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGTGCGcttctt  >  2:472494/1‑140 (MQ=255)                                                                                                                                  tCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGTGCGcttctt  >  1:716259/1‑140 (MQ=255)                                                                                                                                  tCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGTGCGcttctt  >  2:717184/1‑140 (MQ=255)                                                                                                                                  tCCGCTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGAAGCACATCACGTCTTTGACGTGCGcttctt  >  1:360878/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        AGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCACGAGCTGGCTGAAATGCTGCGTACCCGTCTGCTGGGTAAACGTGAACGCGAACTGACCATGGTCTTTGAGTCTTTCGGCTTCAAACACGGTATCCCTATCGATGCAGATTACGTCTTTGACGTGCGCTTCTT  >  NC_000913/3347403‑3347671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑