Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 482,784 A→G R540R (CGT→CGC)  acrB ← multidrug efflux pump RND permease AcrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913482,7840AG100.0% 145.3 / NA 42R540R (CGT→CGC) acrBmultidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/21);  total (21/21)

CGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATG  >  NC_000913/482647‑482919
                                                                                                                                         |                                                                                                                                       
cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCGTGCCGACTACGTTGTTCAGATTCAGCACCAGGTAGCg                                                                                                                                       >  1:192238/1‑140 (MQ=255)
cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCg                                                                                                                                       >  2:166941/1‑140 (MQ=255)
  ttCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGcc                                                                                                                                     <  1:59639/140‑1 (MQ=255)
  ttCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGACGACCACGATGATCAGATACAGCACCAGGTAGCGcc                                                                                                                                     <  1:610534/140‑1 (MQ=255)
   tCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGccc                                                                                                                                    >  1:695125/1‑140 (MQ=255)
        cGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTAc                                                                                                                               >  1:659380/1‑140 (MQ=255)
                       cAGCTGAACCATGGTCATAAACCCGTCCTAGTCCTCAACTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGc                                                                                                                <  1:330873/140‑1 (MQ=255)
                           tGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTAc                                                                                                            <  2:665219/140‑1 (MQ=255)
                                cATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGt                                                                                                       <  2:172544/140‑1 (MQ=255)
                                acaggTCATAAACACGCCCTGGTCCTCATCTCGAAAGAAGGAGCTTGGGAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGt                                                                                                       <  2:313364/137‑1 (MQ=255)
                                  tGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGt                                                                                                       <  2:88569/138‑1 (MQ=255)
                                  tGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGt                                                                                                       >  1:88569/1‑138 (MQ=255)
                                         aaaCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAgt                                                                                              <  1:166941/140‑1 (MQ=255)
                                               gCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTCCGCAGAACACCGCCAACGCTGTCGGTGTAgtggctgt                                                                                        >  1:313364/1‑136 (MQ=255)
                                                 ccTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCGTGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGgtgcgtg                                                                                      >  1:533626/1‑140 (MQ=255)
                                                 ccTGGTCCCCATCTGGCAAGAACGAGCTTGCAATACGCACGAACAGATTGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGgtgcgtg                                                                                      <  2:337495/140‑1 (MQ=255)
                                                    ggTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCGGTCGGTGTAGTGGTGCGTGctc                                                                                   >  1:335033/1‑140 (MQ=255)
                                                     gTCCTCATCTGGCAAGAGGGAGTTTGGCAGACGCAGGAAGAGATAGGCTATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGctct                                                                                  <  2:335033/140‑1 (MQ=255)
                                                     gTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGctct                                                                                  >  1:311701/1‑140 (MQ=255)
                                                     gTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCATACACTGTCGGTGCAGTGGTGCGTGctct                                                                                  >  1:247706/1‑140 (MQ=255)
                                                              tGGCAAGAAGGAGCTTGGCAGACGCTCGAACAGATAGCCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACa                                                                         <  1:323930/140‑1 (MQ=255)
                                                               ggCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATAGCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACAt                                                                        >  1:456988/1‑140 (MQ=255)
                                                               ggCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACAt                                                                        <  2:695125/140‑1 (MQ=255)
                                                               ggCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACAt                                                                        >  2:609685/1‑140 (MQ=255)
                                                                    gaagGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGt                                                                   <  2:311701/140‑1 (MQ=255)
                                                                              ggCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGcc                                                         >  2:54849/1‑140 (MQ=255)
                                                                                      cACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGc                                                 <  2:5941/140‑1 (MQ=255)
                                                                                            cAGATAGGCCATGCCGACCACGATGATCAGATACAACACCAGGTAGCGACCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCttttt                                           <  2:192238/140‑1 (MQ=255)
                                                                                              gATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTa                                         <  1:147944/140‑1 (MQ=255)
                                                                                                tAGGCCATGCCGACCACAATGATCAGATACATCACTAGGTAGCGCCCCTTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTAcc                                       <  2:247706/140‑1 (MQ=255)
                                                                                                      aTGCCGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTcccc                                 >  2:717382/1‑140 (MQ=255)
                                                                                                         ccGACCACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTg                              >  2:552966/1‑140 (MQ=255)
                                                                                                              cACGATGATCAGATACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGc                         >  1:68080/1‑140 (MQ=255)
                                                                                                                           tACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTgg                   <  1:79709/133‑1 (MQ=255)
                                                                                                                           tACAGCACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTgg                   >  2:79709/1‑133 (MQ=255)
                                                                                                                                cACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTgg                   <  2:652583/128‑1 (MQ=255)
                                                                                                                                cACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTgg                   >  1:652583/1‑128 (MQ=255)
                                                                                                                                cACCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCa       >  1:569087/1‑140 (MQ=255)
                                                                                                                                 aCCAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAg      <  2:42662/140‑1 (MQ=255)
                                                                                                                                   cAGGTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCa    <  1:717382/140‑1 (MQ=255)
                                                                                                                                     ggTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCAtg  >  1:53214/1‑140 (MQ=255)
                                                                                                                                     ggTAGCGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCAtg  >  1:590925/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCGTGCTCTTCTCGAACATGCGGTTAAACCAGCCGAAGAAGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATG  >  NC_000913/482647‑482919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑