Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,362,424	T→C	F105L (TTC→CTC)	yhcA →	putative fimbrial chaperone YhcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,362,424	0	T	C	100.0%	250.4 / NA	68	F105L (TTC→CTC)	yhcA	putative fimbrial chaperone YhcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/39);  total (29/39)

GCCTGAAAGCAATAAATCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAATTCCTGGAATGGCGTAAA  >  NC_000913/3362288‑3362558                                                                                                                                         |                                                                                                                                       gCCTGAAAGCAATAAATCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCg                                                                                                                                     >  2:161691/1‑140 (MQ=255)       aaGCAATAAATCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTa                                                                                                                               >  2:682430/1‑140 (MQ=255)           aaTAAATCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTAtt                                                                                                                             <  2:401870/138‑1 (MQ=255)           aaTAAATCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTAtt                                                                                                                             >  1:401870/1‑138 (MQ=255)              aaaTCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTccacc                                                                                                                        <  2:542822/140‑1 (MQ=255)                  cAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCaaaa                                                                                                                    <  1:568584/140‑1 (MQ=255)                   aaTTCGTTTAATCCCGAGTCAGCCCGTTATTGGGGCTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAg                                                                                                                   <  2:347926/140‑1 (MQ=255)                   aaTTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGATGCCGGTCACGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAg                                                                                                                   <  2:216662/140‑1 (MQ=255)                     ttCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGAt                                                                                                                 >  2:156578/1‑140 (MQ=255)                      tCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATg                                                                                                                <  1:705279/140‑1 (MQ=255)                      tCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATg                                                                                                                <  1:702883/140‑1 (MQ=255)                        gTTTAATCCCGACTCAGTCCGTTATTCGTGTTGAAGCCGATCAAGTACTACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGAc                                                                                                              <  2:290162/140‑1 (MQ=255)                        gTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGAc                                                                                                              <  2:561759/140‑1 (MQ=255)                           tAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAag                                                                                                           <  1:719877/140‑1 (MQ=255)                              tCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGagc                                                                                                        <  1:622923/140‑1 (MQ=255)                                ccGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGagcag                                                                                                      <  1:425957/140‑1 (MQ=255)                                ccGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGagcag                                                                                                      <  1:114381/140‑1 (MQ=255)                                ccGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGagcag                                                                                                      <  1:577980/140‑1 (MQ=255)                                        gCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCAAAGTTATCCCACCAAAAGATGCCAAGAGCAGCAGAGTTa                                                                                              >  2:263262/1‑140 (MQ=255)                                         cccGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTAc                                                                                             >  2:667168/1‑140 (MQ=255)                                                 tCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCaa                                                                                     <  2:167758/140‑1 (MQ=255)                                                     gtTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCg                                                                                 >  2:185590/1‑140 (MQ=255)                                                      ttGAAGCCGGTCAAGTCCAACACGTTCGATTTTTACTTCGGGCGACTGCTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGt                                                                                <  2:340101/140‑1 (MQ=255)                                                      ttGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGt                                                                                <  1:407388/140‑1 (MQ=255)                                                      ttGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGt                                                                                <  2:481019/140‑1 (MQ=255)                                                      ttGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGt                                                                                <  2:495461/140‑1 (MQ=255)                                                      ttGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGt                                                                                <  1:667168/140‑1 (MQ=255)                                                      ttGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGt                                                                                <  1:709962/140‑1 (MQ=255)                                                      ttGAAGCCGGTCAAGTACAACAAGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGt                                                                                <  1:270240/140‑1 (MQ=255)                                                        gAAGCCGGTCAAGTACAACAGGCTCCATTTTTACTTCTGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCa                                                                              <  1:205360/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCTATTTTTACATCAGGCGTCTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  2:227521/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCGACCAAAAGTTGATAAGAGCAGCAGAGTTACGGTTTCAATTCGTCAGGa                                                                           >  2:196878/1‑140 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  1:682430/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  1:705141/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  2:474503/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  2:17272/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  2:406385/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCCATTTTTACTTCAGGGGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  1:280275/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAGGTTCCATCTTTCCTTCAGGTGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  1:263262/140‑1 (MQ=255)                                                           gCCGGTCAAGTACAACAAGTTCAATTTTTACTTCACGCGCCTGTTCCTTTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGa                                                                           <  2:208250/140‑1 (MQ=255)                                                            ccGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGAt                                                                          <  2:692025/140‑1 (MQ=255)                                                             cGGTCAAGAACAACAGGTTCGAATTTTACTTCAGGCGACGGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATc                                                                         <  2:336725/140‑1 (MQ=255)                                                                tCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTAc                                                                      <  1:699050/140‑1 (MQ=255)                                                                tCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTAc                                                                      <  1:185590/140‑1 (MQ=255)                                                                    gTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGt                                                                  >  2:365255/1‑140 (MQ=255)                                                                             gTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCa                                                         >  1:653101/1‑140 (MQ=255)                                                                              ttCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCAc                                                        >  1:371884/1‑140 (MQ=255)                                                                                             aGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCg                                         >  1:493045/1‑140 (MQ=255)                                                                                              ggCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCgg                                        >  2:602144/1‑140 (MQ=255)                                                                                               gCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGa                                       >  2:601806/1‑140 (MQ=255)                                                                                               gCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGa                                       >  2:697395/1‑140 (MQ=255)                                                                                                cGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGaa                                      <  1:161691/140‑1 (MQ=255)                                                                                                    tGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGc                                  >  2:614877/1‑140 (MQ=255)                                                                                                        ccTCTGCAATCAGAAGAGCTGATACGCGTTACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCgaga                              <  1:316659/140‑1 (MQ=255)                                                                                                         ctctGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCgagag                             >  1:680011/1‑140 (MQ=255)                                                                                                         ctctGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCAGTACTGAGCCACCCGGCTTCCCTGCCGGAAGAGCgagag                             >  2:291692/1‑140 (MQ=255)                                                                                                         ctctGCAATCAGAAGAGATGAACCGCGTAACTCTCAAAGTTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCgagag                             <  2:205065/140‑1 (MQ=255)                                                                                                            tGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGAcc                          >  2:142585/1‑140 (MQ=255)                                                                                                            tGCAATCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGAcc                          >  2:589244/1‑140 (MQ=255)                                                                                                                aTCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGa                      >  1:158202/1‑140 (MQ=255)                                                                                                                aTCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTAACTGTACTGGTCCGCCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGa                      >  2:206578/1‑140 (MQ=255)                                                                                                                aTCAGAAGAGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTAACTGTACTGATCCACACGGCTTCCCTTCCGGAAGAGCGAGAGACCTGGa                      >  2:316659/1‑140 (MQ=255)                                                                                                                   agaagaGCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAAt                   >  1:695497/1‑140 (MQ=255)                                                                                                                        agCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAATTCCTg              >  2:634020/1‑140 (MQ=255)                                                                                                                        agCTGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTAATCCACCCTGCTTCCCTGCCGGAAGCGCGAAAGACCTGGAAATTCCTg              >  2:298779/1‑140 (MQ=255)                                                                                                                           tGAAACGCGTAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATACACCCGGCATCCATGCCGGAAGAGCTAGAGACCTGGAAATTCCTGGaa           >  2:186865/1‑140 (MQ=255)                                                                                                                                    tAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAATTCCTGGAATGGCGTaaa  >  2:171854/1‑140 (MQ=255)                                                                                                                                    tAACGCTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCAGAAGAGCCAGAGACCTCGAAACTCCTGGAATGGCGTaaa  >  2:268724/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GCCTGAAAGCAATAAATCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGTCAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAATTCCTGGAATGGCGTAAA  >  NC_000913/3362288‑3362558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑