Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,386,282 T→C E636G (GAA→GGA)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,386,2820TC100.0% 137.0 / NA 39E636G (GAA→GGA) aaeBaromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/14);  total (25/14)

CATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTCTGCTGTGCGGCG  >  NC_000913/3386169‑3386415
                                                                                                                 |                                                                                                                                     
cATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAg                                                                                                             <  1:105259/140‑1 (MQ=255)
cATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAg                                                                                                             <  1:484975/140‑1 (MQ=255)
    gCTAAGTATAGCCTTGAAAAACCCGACGTTTTTGGCGGCGGTTTTGACTTACCTATCGGTCAACGCATGTTGATGCTTAGGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGc                                                                                                         <  2:674826/140‑1 (MQ=255)
        ggTATAGCCATGAAAAAACCGACGCGTTTGGCGTCGGGTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAg                                                                                                     <  1:291836/139‑1 (MQ=255)
                   gAAAAAACCGACGCTTTTGGCGTCGGTTATGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGt                                                                                          >  2:620576/1‑140 (MQ=255)
                    aaaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa                                                                                         >  1:728273/1‑140 (MQ=255)
                    aaaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa                                                                                         >  2:157443/1‑140 (MQ=255)
                    aaaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGATCCCGTCAACTGCGGTGGCGCTTGCAAGATGCGTAGCTTTTCCTGGtc                                                                                         >  2:291836/1‑139 (MQ=255)
                                       cGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGc                                                                      <  1:157443/140‑1 (MQ=255)
                                         tCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGtt                                                                              <  1:81157/130‑1 (MQ=255)
                                         tCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGtt                                                                              >  2:81157/1‑130 (MQ=255)
                                         tCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGACGCGTAGCCTTTCCTGGTAGATATCCAGTTCTTCCAGCaa                                                                    >  2:301657/1‑140 (MQ=255)
                                                      aaCTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTAc                                                       >  2:145733/1‑140 (MQ=255)
                                                       ccTATCGGTCAACGCATGTTGATACTTATGGGGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTAcc                                                      <  1:718697/139‑1 (MQ=255)
                                                           tCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACcgacg                                                  >  2:127838/1‑140 (MQ=255)
                                                            cGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACt                                                                  >  2:89988/1‑123 (MQ=255)
                                                            cGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACt                                                                  <  1:89988/123‑1 (MQ=255)
                                                                   cGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTat                                          >  2:429923/1‑140 (MQ=255)
                                                                    gCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTatc                                         <  1:391466/140‑1 (MQ=255)
                                                                           gATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTa                                  <  2:452795/140‑1 (MQ=255)
                                                                           gATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTa                                  <  1:127838/140‑1 (MQ=255)
                                                                             tACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACg                                >  1:402031/1‑140 (MQ=255)
                                                                             tACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACg                                >  1:458802/1‑140 (MQ=255)
                                                                               cTTATGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACggg                              <  1:620576/140‑1 (MQ=255)
                                                                                  aTGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAg                           <  2:728273/140‑1 (MQ=255)
                                                                                   tGGAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGa                          >  2:66834/1‑140 (MQ=255)
                                                                                    ggAGCATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCTAAAGTACGGACGACGTTTATCGTCGCTACGGGCAGat                         >  1:191421/1‑140 (MQ=255)
                                                                                    ggAGCATCCCCGCCAGCCGATTCACTGGTGCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGat                         <  1:301657/140‑1 (MQ=255)
                                                                                        cATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATca                     >  2:13687/1‑140 (MQ=255)
                                                                                        cATCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATca                     >  2:307104/1‑140 (MQ=255)
                                                                                          tCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATcaca                   >  1:465528/1‑140 (MQ=255)
                                                                                          tCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATcaca                   >  2:559218/1‑140 (MQ=255)
                                                                                          tCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATcaca                   >  1:657477/1‑140 (MQ=255)
                                                                                          tCCCCGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATcaca                   >  1:130665/1‑140 (MQ=255)
                                                                                           ccccGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACAt                  >  2:102152/1‑140 (MQ=255)
                                                                                             ccGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGCCCAAAGTACCGAAGACATTTATCATCGCTAGGGGCAGATATCACATgg                >  2:200761/1‑140 (MQ=255)
                                                                                              cGCCAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGt               >  2:633770/1‑140 (MQ=255)
                                                                                                ccAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGc                             >  2:397733/1‑124 (MQ=255)
                                                                                                ccAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGc                             <  1:397733/124‑1 (MQ=255)
                                                                                                ccAGCCGATTTACCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTct             >  2:542268/1‑140 (MQ=255)
                                                                                                           aCCGGTCCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTCTGCTGTgcggcg  <  1:562165/140‑1 (MQ=255)
                                                                                                                 |                                                                                                                                     
CATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTCTGCTGTGCGGCG  >  NC_000913/3386169‑3386415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑