| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,420,535 | T→C | I134I (ATT→ATC) | yhdX → | putative ABC transporter membrane subunit YhdX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,420,535 | 0 | T | C | 94.7% | 126.5 / NA | 38 | I134I (ATT→ATC) | yhdX | putative ABC transporter membrane subunit YhdX |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (24/12); minor base G (1/1); total (25/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGCTACTGGTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGG > NC_000913/3420403‑3420668 | tACGCTACTGGTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCgc > 2:709307/1‑140 (MQ=255) aCGCTACTGGTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCgct > 2:399591/1‑140 (MQ=255) aCGCTACTGGTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCgct > 2:104270/1‑140 (MQ=255) tACTGGTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGctgctg > 2:529016/1‑140 (MQ=255) tGGTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCaa > 1:486127/1‑140 (MQ=255) gTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCTGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAAt < 1:504453/140‑1 (MQ=255) ttCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAAtct < 2:457598/140‑1 (MQ=255) tGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAAtcttct > 2:469689/1‑140 (MQ=255) tgtattgtgtTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTa > 2:720042/1‑140 (MQ=255) tCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCgtg > 2:575435/1‑140 (MQ=255) tCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCgtg > 2:156394/1‑140 (MQ=255) tCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCgtg > 2:115649/1‑140 (MQ=255) tCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCgtg > 2:721542/1‑140 (MQ=255) tCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCgtg > 2:92893/1‑140 (MQ=255) tCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTACGTAATATGCCCTCGCTGCTGCAAATCTTCCTCTGGTACTTTGCCgtg > 2:203781/1‑140 (MQ=255) ttttGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGTAAAAATTCTTCTGGTACATTGCCGTGTTGCGCAAt > 2:341044/1‑140 (MQ=255) ttttGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAAt < 2:544346/140‑1 (MQ=255) ggCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGcc < 1:720042/140‑1 (MQ=255) cttcttTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCg < 1:529016/140‑1 (MQ=255) cttcttTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCg < 1:399591/140‑1 (MQ=255) gTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGAccctgtctc > 1:104946/1‑133 (MQ=255) cTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGc > 2:536511/1‑140 (MQ=255) tGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTGTTCTGGTAATTTACCGGGTTGCGCATTTTGCCCGGACCACGCCAGGCt > 2:254529/1‑140 (MQ=255) tGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCt > 2:392194/1‑140 (MQ=255) agaCTTTCGGATAACTGCCTGCTACGAAAGCTTTCCACACTTTATATTAAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAg < 2:193378/140‑1 (MQ=255) aCTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAgcg > 2:611743/1‑140 (MQ=255) ttCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGt < 1:92893/140‑1 (MQ=255) cTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTgg < 1:688255/140‑1 (MQ=255) cTGGCCGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTgg < 2:682053/140‑1 (MQ=255) gctgctACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCt > 1:713192/1‑140 (MQ=255) cGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAg > 2:60128/1‑140 (MQ=255) tCCACAATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTgg < 1:709307/140‑1 (MQ=255) acaATTTATATTGAGATCTTCCCTACTATGCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCt < 1:203781/140‑1 (MQ=255) caATTTATATTGAGATCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCtt > 2:672291/1‑140 (MQ=255) gagaTCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTc > 2:20860/1‑140 (MQ=255) gagaTCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTc > 2:13699/1‑140 (MQ=255) agaTCTTCCGTAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCa < 1:575435/140‑1 (MQ=255) tAATATCCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTgg > 1:58584/1‑140 (MQ=255) | TACGCTACTGGTTTCTGCATTGTGTATTGTGTTCGCTTCTGTTTTGGGCTTCTTTATCGGTCTGGCGAGACTTTCGGATAACTGGCTGCTACGAAAGCTTTCCACAATTTATATTGAGATCTTCCGTAATATTCCCCCGCTGCTGCAAATCTTCTTCTGGTACTTTGCCGTGTTGCGCAATTTGCCCGGACCACGCCAGGCTGTGAGCGCGTTTGATCTGGCCTTTTTGAGCAATCGTGGGCTTTATATTCCGTCACCGCAGCTGG > NC_000913/3420403‑3420668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |