| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,457,425 | T→C | V350A (GTT→GCT) | gspD → | Type II secretion system protein GspD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,457,425 | 0 | T | C | 98.0% | 175.5 / NA | 50 | V350A (GTT→GCT) | gspD | Type II secretion system protein GspD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (26/23); minor base A (1/0); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATGGATAACGTCGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGTTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGAT > NC_000913/3457296‑3457561 | cgATGGATAACGTCGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAatc > 2:437608/1‑140 (MQ=255) agggATAACGTCGCAGTTACCGCCGCTGACCAGACTATCTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAatcat < 1:204760/138‑1 (MQ=255) aacggccGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTg < 2:462016/134‑1 (MQ=255) cGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGTACATTCGCGGTGAACAGGTGCTGGCAGAGGAAATCATCGTTGAAGTTc > 2:274693/1‑140 (MQ=255) cGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTc > 2:494620/1‑140 (MQ=255) cGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTc > 1:385919/1‑140 (MQ=255) cGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGAGCACAGGGGCTGGCTGAGGCAATCATCGTTGAAGTTc > 1:229052/1‑140 (MQ=255) gCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCAACGTTGAAGTTCa > 2:533484/1‑140 (MQ=255) ccATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGGACAGGTGATGGATGAGGCAATCATCGTTGAAGTTCAg > 1:262254/1‑140 (MQ=255) cATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAgg > 1:99252/1‑140 (MQ=255) aTTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGa < 1:496839/140‑1 (MQ=255) aTGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGa < 2:608255/140‑1 (MQ=255) aaCAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTa > 2:158220/1‑140 (MQ=255) aCTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCt > 2:606245/1‑140 (MQ=255) aCTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCt > 1:526615/1‑140 (MQ=255) ctctctGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCg < 1:500784/137‑1 (MQ=255) ctctctGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCg > 2:500784/1‑137 (MQ=255) ctGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCa < 1:384781/140‑1 (MQ=255) tGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCaa > 2:727367/1‑140 (MQ=255) tGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCaa > 2:488615/1‑140 (MQ=255) tGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCAACGTTGAAGTTCAGGATGGAAATGGACTAAACCTCAGCGTGCaa < 1:533484/140‑1 (MQ=255) aTTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCACCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGc < 1:130311/140‑1 (MQ=255) ttACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGCAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCg > 2:454959/1‑140 (MQ=255) aCCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAAc > 2:94193/1‑140 (MQ=255) cAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGt > 1:577918/1‑140 (MQ=255) cAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGt < 2:577918/140‑1 (MQ=255) cgtccAGGAAAAACTCGACACGCTAATTGCGCGTCTGGATATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGgcg < 2:262254/140‑1 (MQ=255) gtccAGGAAAACCTCGCCCCGGTAATTGCGCGTCTGGCCATCCGCCGTGCACAGGTGCTGGCTGAGGAATTCATCGTTAAAGTTCAGGATGGAAATGGCCT‑AACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGca < 2:99252/140‑1 (MQ=255) tccAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGca > 1:624073/1‑140 (MQ=255) ccAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGcac > 2:429472/1‑140 (MQ=255) ccAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGcac < 1:559832/140‑1 (MQ=255) ccAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGcac > 2:559832/1‑140 (MQ=255) aaaaCTCGCCACGGTCATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAAt < 1:274693/140‑1 (MQ=255) aaaCTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAAtt < 1:488615/140‑1 (MQ=255) tCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTAcc > 2:644229/1‑140 (MQ=255) ccACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAAt > 2:380234/1‑140 (MQ=255) ccACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAAt < 1:494620/140‑1 (MQ=255) tGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTAc < 2:679825/140‑1 (MQ=255) tGGACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGAttttt > 1:59286/1‑140 (MQ=255) ggACATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTa > 2:689619/1‑140 (MQ=255) aCATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAAc < 1:158220/140‑1 (MQ=255) aCATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAAc < 1:727367/140‑1 (MQ=255) cATTCGCCGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACg < 1:167055/140‑1 (MQ=255) ccGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTgcgc > 1:530125/1‑140 (MQ=255) cGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCa < 2:526615/140‑1 (MQ=255) cGTGCACAGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCa < 1:689619/140‑1 (MQ=255) cGTGCACAGGTGCTGGCTGAGGAAATCATCGCTGACGTTAAGGATGGACATGGACTAAACCTCGGCGTGCAATGGCCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCa < 2:229052/140‑1 (MQ=255) cacaGGTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGgt > 2:543541/1‑140 (MQ=255) gTGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGc < 2:530125/140‑1 (MQ=255) tGCTGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCt > 1:82748/1‑140 (MQ=255) tGGCTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGAt < 1:429472/140‑1 (MQ=255) | CGATGGATAACGTCGCCATTACCGCCGATGAACAGACTAACTCTCTGGTCATTACCGCTGACCAGTCCGTCCAGGAAAAACTCGCCACGGTAATTGCGCGTCTGGACATTCGCCGTGCACAGGTGCTGGTTGAGGCAATCATCGTTGAAGTTCAGGATGGAAATGGACTAAACCTCGGCGTGCAATGGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGAT > NC_000913/3457296‑3457561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |