Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	493,451	A→G	T454A (ACC→GCC)	dnaX →	DNA polymerase III subunit tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	493,451	0	A	G	100.0%	111.6 / NA	32	T454A (ACC→GCC)	dnaX	DNA polymerase III subunit tau
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/15);  total (17/15)

CCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTG  >  NC_000913/493317‑493582                                                                                                                                       |                                                                                                                                    ccagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGAt                                                                                                                                >  1:48990/1‑140 (MQ=255)  cagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATc                                                                                                                               <  1:417580/140‑1 (MQ=255)            cTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGgc                                                                                                                     <  1:325739/140‑1 (MQ=255)              ggcggcGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGgcgc                                                                                                                   >  2:157702/1‑140 (MQ=255)                   cgcgcCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCg                                                                                                              <  1:95743/140‑1 (MQ=255)                                gCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGgcgc                                                                                                 >  2:556011/1‑140 (MQ=255)                                gCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGgcgc                                                                                                 >  2:140621/1‑140 (MQ=255)                                     gcgTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGaa                                                                                            >  2:107681/1‑140 (MQ=255)                                            gggAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAgcgc                                                                                     <  1:31223/140‑1 (MQ=255)                                                       aaGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCaaaaaa                                                                          >  2:494219/1‑140 (MQ=255)                                                         gCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAaaga                                                                        >  1:558441/1‑140 (MQ=255)                                                             aaaaGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCg                                                                    >  2:377992/1‑140 (MQ=255)                                                               aaGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTa                                                                  <  2:48990/140‑1 (MQ=255)                                                                           gCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGc                                                      <  1:391033/140‑1 (MQ=255)                                                                            cAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAGAGCGCCAGCCAGAAGAGAAGCATATCGCTGGAAGGCg                                                     >  1:215825/1‑140 (MQ=255)                                                                            cAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCg                                                     <  1:494219/140‑1 (MQ=255)                                                                              gCCGCTACCCGCGCGCGGCCGGGGAATAACGTTGCTCTGCAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGAc                                                   <  2:215825/140‑1 (MQ=255)                                                                               ccGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGAcc                                                  <  1:107681/140‑1 (MQ=255)                                                                                   tACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGag                                                         <  1:115745/129‑2 (MQ=255)                                                                                   tACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGaa                                                         >  2:115745/1‑129 (MQ=255)                                                                                           cgcgGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATg                                      <  2:610283/140‑1 (MQ=255)                                                                                             cgGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATgca                                    <  1:556011/140‑1 (MQ=255)                                                                                             cgGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATgca                                    <  1:377992/140‑1 (MQ=255)                                                                                             cgGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATgca                                    >  1:371506/1‑140 (MQ=255)                                                                                                              tgcgctgGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCg                   >  2:103937/1‑140 (MQ=255)                                                                                                                cgctgGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGGGACCACTACGGTGAAGCAGCAGAAAGAAGTGGTCGcc                 >  1:202489/1‑140 (MQ=255)                                                                                                                cgctgGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGcc                 >  1:689482/1‑140 (MQ=255)                                                                                                                cgctgGAAAGACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTAACGCTGGAAGGCGCCAAATCCGGTGATGCAGCAAAAAGAAGTGGTCGcc                 >  2:338967/1‑140 (MQ=255)                                                                                                                         gACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAg        <  2:371506/140‑1 (MQ=255)                                                                                                                         gACTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAg        <  1:157702/140‑1 (MQ=255)                                                                                                                          aCTGGCTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAgg       >  2:517597/1‑140 (MQ=255)                                                                                                                               cTTCGGTCGCCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTTATGCAGCAAAAAGAAGGGGTCGCCACGCCGAAGGCGCTg  >  2:285182/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    CCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTG  >  NC_000913/493317‑493582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑