Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,463,197 A→G T92A (ACA→GCA)  gspK → Type II secretion system protein GspK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,463,1970AG100.0% 146.3 / NA 42T92A (ACA→GCA) gspKType II secretion system protein GspK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/17);  total (25/17)

GCAATATGATATTGAACTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATACAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGTGCGCTG  >  NC_000913/3463064‑3463313
                                                                                                                                     |                                                                                                                    
gCAATATGATATTGAACTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGa                                                                                                                >  1:728555/1‑140 (MQ=255)
     atGATATTGAACTGGTGGAGAACCTTGCGTTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTgg                                                                                                           <  1:244179/140‑1 (MQ=255)
        atatTGAACTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAg                                                                                                        >  1:166745/1‑140 (MQ=255)
         tatTGAACTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGt                                                                                                       >  1:71473/1‑140 (MQ=255)
              aaCTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGtt                                                                                                      >  1:165948/1‑136 (MQ=255)
              aaCTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGtt                                                                                                      <  2:165948/136‑1 (MQ=255)
              aaCTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTAcgc                                                                                                  >  1:27196/1‑140 (MQ=255)
              aaCTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGATGGTAGATGGCAGTGCAATGAAGTGGCAGTTAcgc                                                                                                  >  2:213714/1‑140 (MQ=255)
               aCTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTAcgcg                                                                                                 <  2:166745/140‑1 (MQ=255)
               aCTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTAcgcg                                                                                                 <  2:129264/140‑1 (MQ=255)
                cTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCTGCTGGAAGATGGCAATGCAGTCAAGTGGCAGTTACGCGa                                                                                                >  2:281116/1‑140 (MQ=255)
                    cGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGcc                                                                                            <  2:27196/140‑1 (MQ=255)
                    cGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGcc                                                                                            <  2:116902/140‑1 (MQ=255)
                       agaAACTTGCGCTTGTCAGCCTCACCCAGCACGTTAAGGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAg                                                                                         <  1:210094/140‑1 (MQ=255)
                         aaaCTTGCGCTTGCCAGCCTCCCCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCa                                                                                       <  2:610926/140‑1 (MQ=255)
                         aaaCTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCa                                                                                       <  2:73304/140‑1 (MQ=255)
                         aaaCTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCa                                                                                       <  1:380437/140‑1 (MQ=255)
                                          ccTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATg                                                                      >  2:672809/1‑140 (MQ=255)
                                               cccAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTa                                                                 >  2:603366/1‑140 (MQ=255)
                                                         tAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAAtct                                                       >  2:621124/1‑140 (MQ=255)
                                                                aaCGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTgacga                                                >  2:44168/1‑140 (MQ=255)
                                                                        acagacCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAACCTCAATGCGTTAGCAAAAATCACTGACGACCCGCTgg                                        >  1:314712/1‑140 (MQ=255)
                                                                          agacCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCt                                      >  2:561389/1‑140 (MQ=255)
                                                                            acCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAGTCTCTGTCGACCCGCTGGCTTc                                    >  1:196337/1‑140 (MQ=255)
                                                                            acCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCCTc                                    >  1:609460/1‑140 (MQ=255)
                                                                                        cagTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTccc                        >  1:715822/1‑140 (MQ=255)
                                                                                        cagTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTccc                        >  1:77270/1‑140 (MQ=255)
                                                                                          gTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAATATCTCTGTCGAGCCGCTGGCTGCCCCTGATTTCCCCg                      >  1:231947/1‑140 (MQ=255)
                                                                                          gTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCg                      <  2:609460/140‑1 (MQ=255)
                                                                                                  cacaGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTa              >  1:34232/1‑140 (MQ=255)
                                                                                                     aGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTc           >  1:701804/1‑140 (MQ=255)
                                                                                                     aGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTc           >  1:505578/1‑140 (MQ=255)
                                                                                                     aGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTc           >  1:621380/1‑140 (MQ=255)
                                                                                                     aGCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACCCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGCATTc           >  2:27778/1‑140 (MQ=255)
                                                                                                      gCCACAACAGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCa          <  1:561389/140‑1 (MQ=255)
                                                                                                        cacaACAGTTGCAGCTGCAAGAAGGCAATGCCGTGCAGTCGCAGTTACCCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGt        <  2:196337/140‑1 (MQ=255)
                                                                                                         acaacaGTTGCAGCTGGGAGATGGCAATGCAATGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGTg       <  2:308918/140‑1 (MQ=255)
                                                                                                          caacaGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTGTGACGACCGGCTGGCTGCCCCTGATGTCCCGGCCCAGGTATTCAGTgc      >  2:198923/1‑140 (MQ=255)
                                                                                                          caacaGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGTgc      >  2:146239/1‑140 (MQ=255)
                                                                                                           aacaGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGTgcg     <  1:183432/140‑1 (MQ=255)
                                                                                                              aGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGTGCgctg  <  1:44168/140‑1 (MQ=255)
                                                                                                              aGTTGCAGCTGGAAGATGGCAATGCAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGTGCgctg  <  1:603366/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                    
GCAATATGATATTGAACTGGCGGAGAAACTTGCGCTTGCCAGCCTCACCCAGGACGTTAAAGATAACGACAGACAGACCACGTTGCAGCAGTACTGGGCACAGCCACAACAGTTGCAGCTGGAAGATGGCAATACAGTGAAGTGGCAGTTACGCGATGCCCAGCACTGTTTTAATCTCAATGCGTTAGCAAAAATCTCTGACGACCCGCTGGCTTCCCCTGATTTCCCCGCCCAGGTATTCAGTGCGCTG  >  NC_000913/3463064‑3463313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑