| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,518,153 | C→T | G112S (GGC→AGC) | aroB ← | 3‑dehydroquinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,518,153 | 0 | C | T | 97.9% | 157.4 / NA | 47 | G112S (GGC→AGC) | aroB | 3‑dehydroquinate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (24/22); minor base G (1/0); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTCAGGGAGGAT > NC_000913/3518019‑3518285 | cgcgCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAAGGGAGACCACGCTGATAGCTCGGCGCCGCGAAGCTGGTCa > 2:204267/1‑140 (MQ=255)cgcgCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAAGGGACATCAGGCTGATACCTCGATGCCGCGAAGCTGGTCa > 2:357211/1‑140 (MQ=255)cgcgCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCa > 1:127343/1‑140 (MQ=255)cgcgCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCa > 1:555631/1‑140 (MQ=255)cgcgCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTGGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCa > 2:327769/1‑140 (MQ=255) ttACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAAGGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTAcgcc < 1:357211/140‑1 (MQ=255) aGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACgccgccgcc > 2:734023/1‑140 (MQ=255) aGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTAATAACTCGACGCCGCGAAGCTGGTCTGATCACCCACTACgccgccgcc > 2:284709/1‑140 (MQ=255) ggggATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCTACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCa > 2:172104/1‑140 (MQ=255) ggggATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCa > 2:576572/1‑140 (MQ=255) gggATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCaa > 2:116891/1‑140 (MQ=255) ttGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGccac < 2:555631/140‑1 (MQ=255) aCCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAg > 2:626425/1‑140 (MQ=255) aCCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAg > 2:29506/1‑140 (MQ=255) aCCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCGGGTAAGAACGCCCACTACGCCGCCGCCAAGCGCCACCAg > 2:280775/1‑140 (MQ=255) aCGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATg < 1:29506/140‑1 (MQ=255) cTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCGGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTaaca > 1:546955/1‑140 (MQ=255) gCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTaacaac < 2:472602/140‑1 (MQ=255) gCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTaacaac < 1:734023/140‑1 (MQ=255) gCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTaacaac < 1:116891/140‑1 (MQ=255) gCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTaacaac > 1:472602/1‑140 (MQ=255) cGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCCCGCCCATGCTGTTTTTGTAACAACg > 1:243831/1‑140 (MQ=255) aCAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGcc > 2:548758/1‑140 (MQ=255) aCAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGcc < 2:418559/140‑1 (MQ=255) cgtcgtCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGa < 1:462075/140‑1 (MQ=255) cgtcgtCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGa < 1:633881/140‑1 (MQ=255) tcgtcgGGACTTGAATGCAACGAACACCGCGGTGACAACTCGCCGCCGAGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACg < 2:244309/140‑1 (MQ=255) tcgGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTa < 1:719175/140‑1 (MQ=255) tcgGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTa > 1:704702/1‑140 (MQ=255) gACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGa > 1:69374/1‑140 (MQ=255) ttGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTa > 2:73190/1‑140 (MQ=255) gaaACGGACACCGCGTTGATAAATCACCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCa < 1:204267/140‑1 (MQ=255) gaaACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCa < 2:69374/140‑1 (MQ=255) aaaCGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCC‑CCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAgg > 1:138481/1‑140 (MQ=255) aaCGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAgg > 1:406558/1‑140 (MQ=255) aaCGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAgg < 2:127343/140‑1 (MQ=255) aaCGGACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAgg < 1:576572/140‑1 (MQ=255) ggACACCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCtt < 1:548758/140‑1 (MQ=255) cacCGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAGAGAGGGGATCGAGAACAGCCAGGCTTTta > 1:237626/1‑140 (MQ=255) ccGCGCTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTtata < 2:406558/140‑1 (MQ=255) cgcTGATAACTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTCTAACAACGACGTGAAGACGGTATCGCGTACAGCCAGGCTTTTATACTg > 2:236746/1‑140 (MQ=255) aaCTCGCCGCCGCGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCg < 2:76445/140‑1 (MQ=255) cTCGCCGCCGCGAAGCTGGTCAGATCGCCAACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTc < 1:327769/140‑1 (MQ=255) gccgccgcGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTCAgg < 1:694463/140‑1 (MQ=255) gccgcGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTCAgg > 2:56168/1‑137 (MQ=255) gccgcGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTCAgg < 1:56168/137‑1 (MQ=255) gccgcGAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTCAGggag > 2:54189/1‑140 (MQ=255) gcgAAGCTGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTCAGGGAGGAt < 1:620485/140‑1 (MQ=255) | CGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCATGCGGTTTTTGTAACAACGCCGTAAAGACGGTATCGAGTACAGCCAGGCTTTTATACTGCTCGCCGTCAGGGAGGAT > NC_000913/3518019‑3518285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |