Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,573,754	C→T	intergenic (‑252/+21)	glgB ← / ← asd	1,4‑alpha‑glucan branching enzyme/aspartate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,573,754	0	C	T	100.0%	211.8 / NA	63	intergenic (‑252/+21)	glgB/asd	1,4‑alpha‑glucan branching enzyme/aspartate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/27);  total (36/27)

TCACTTCAGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCCCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTCCCATATTCAGCTTACGCAGGCGGC  >  NC_000913/3573617‑3573890                                                                                                                                          |                                                                                                                                         tCACTTCAGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGctcc                                                                                                                                        >  1:696450/1‑140 (MQ=255) tCACTTCAGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACAGCTGCGCTTGCTCGTGTGTTACGCACGAACATGGGCGGCATCGCGctcc                                                                                                                                        >  2:200856/1‑140 (MQ=255)     ttCAGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGAt                                                                                                                                    >  2:50002/1‑140 (MQ=255)       cAGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGAttt                                                                                                                                  <  2:681679/140‑1 (MQ=255)       cAGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGAttt                                                                                                                                  <  1:391087/140‑1 (MQ=255)        aGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTa                                                                                                                                 <  2:47203/140‑1 (MQ=255)        aGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTa                                                                                                                                 <  2:388926/140‑1 (MQ=255)             aGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGaa                                                                                                                            >  1:150373/1‑140 (MQ=255)               gCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATa                                                                                                                          <  1:50002/140‑1 (MQ=255)               gCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATa                                                                                                                          >  2:382341/1‑140 (MQ=255)                   tgAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACT‑CCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGAt                                                                                                                     >  1:576331/1‑140 (MQ=255)                      aTACTCATGTATTCAGCCACCCCTAAAGAATAGCCTATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTa                                                                                                                   <  2:275663/140‑1 (MQ=255)                         cTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGc                                                                                                                >  2:498445/1‑140 (MQ=255)                         cTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGc                                                                                                                >  1:619425/1‑140 (MQ=255)                            aTGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAg                                                                                                             <  1:53498/140‑1 (MQ=255)                             tGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGt                                                                                                            >  2:533418/1‑140 (MQ=255)                             tGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGt                                                                                                            >  1:100300/1‑140 (MQ=255)                                 ttCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGAc                                                                                                        >  1:713504/1‑140 (MQ=255)                                    aGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGaa                                                                                                     >  1:718885/1‑140 (MQ=255)                                      ccACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGc                                                                                                   >  2:156663/1‑140 (MQ=255)                                                aGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCa                                                                                         >  1:380605/1‑140 (MQ=255)                                                    tAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATCAAGATTACGCCCGTTGACGAAGCATCCGACGCAGCgg                                                                                     >  1:284128/1‑140 (MQ=255)                                                     aGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAGAGATTACTCCAGTTGACGAAGAATCCGACGCAGCGGc                                                                                    >  1:279126/1‑140 (MQ=255)                                                            gCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCgg                                                                             <  2:588702/140‑1 (MQ=255)                                                            cagctATTTAACTCCCGTTAAACCATGAAACTTCTCCGCCTACTCCTGCATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCgg                                                                             <  2:205772/137‑1 (MQ=255)                                                              tctATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGcc                                                                           <  1:13738/140‑1 (MQ=255)                                                               ctATTTAACTCCCGGTAAATCATGAAACATCGGCGTTTACTCCTGTATAACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGccc                                                                          <  2:318187/140‑1 (MQ=255)                                                                tATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGcccc                                                                         <  2:701264/140‑1 (MQ=255)                                                                      aCTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGCAGCAGCGGCTCCGCGTCCCCCCAcag                                                                   >  1:317804/1‑140 (MQ=255)                                                                       cTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCAcagc                                                                  >  2:525147/1‑140 (MQ=255)                                                                       cTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCAcagc                                                                  >  2:442099/1‑140 (MQ=255)                                                                        tCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCATTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGATGAACCATTCGACGCAGCGGCTCCGCGGCCCCCCAcagca                                                                 >  2:284853/1‑140 (MQ=255)                                                                          ccGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGAGGCACCGGCTCCGCCGCCCCCCAcagcctg                                                               >  1:205772/1‑137 (MQ=255)                                                                          ccGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACagcagc                                                               >  1:411212/1‑140 (MQ=255)                                                                          ccGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACagcagc                                                               >  2:302/1‑140 (MQ=255)                                                                            ggTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAACAGCTg                                                             >  1:551537/1‑140 (MQ=255)                                                                             gTAAATCATGAAACACCTGCGCTTACTCCTGTATTACTCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTgg                                                            <  1:198852/140‑1 (MQ=255)                                                                                aaTCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCg                                                         <  2:380605/140‑1 (MQ=255)                                                                                  tCATGAAACATCTGCGCTTACTCCTCTATTACGCATTGACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGcc                                                       <  1:284853/140‑1 (MQ=255)                                                                                   cATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACCGGGGCGGCCTCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGccc                                                      <  2:307495/140‑1 (MQ=255)                                                                                   cATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGccc                                                      <  1:533418/140‑1 (MQ=255)                                                                                   cATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGccc                                                      <  2:150373/140‑1 (MQ=255)                                                                                   cATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGccc                                                      <  1:525147/140‑1 (MQ=255)                                                                                              tGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCCCGGCCCCCCACTGCAGCTGGTAGCCCACGGTAAAGGc                                           >  1:340103/1‑140 (MQ=255)                                                                                                     aCTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAACAGCTGGTCGCCCACGGTAAAGGCTGACAgg                                    <  2:551537/140‑1 (MQ=255)                                                                                                            tATTACGCACTAACAGGTGCGGCATCGCGCTACAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCTACGGTAAAGGCTGACAGGAACTCTg                             <  2:284128/140‑1 (MQ=255)                                                                                                            tATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTg                             <  1:498445/140‑1 (MQ=255)                                                                                                            tATTACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTg                             <  2:576331/140‑1 (MQ=255)                                                                                                            tATTACGCACTAACAGGCGCGGCATCGCGCTACAGGTTTTATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTg                             <  2:262655/140‑1 (MQ=255)                                                                                                               tACGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTCACAGGAAcctgtctc                          >  1:324505/1‑133 (MQ=255)                                                                                                                aCGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTcc                         <  1:382341/140‑1 (MQ=255)                                                                                                                aCGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTcc                         <  2:718885/140‑1 (MQ=255)                                                                                                                 cGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTTGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTccc                        >  1:319571/1‑140 (MQ=255)                                                                                                                 cGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTccc                        >  1:20917/1‑140 (MQ=255)                                                                                                                 cGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTccc                        >  1:560031/1‑140 (MQ=255)                                                                                                                 cGCACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGAAAAGGCTGACAGGAACTCTGGTccc                        >  1:320621/1‑140 (MQ=255)                                                                                                                   cACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGGCGCCCACCGTAAAGACTGACATGAACTCTGGTCCCat                      >  1:313907/1‑140 (MQ=255)                                                                                                                   cACTAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGCCGCCCACGGTAGAGTCTGACACGAACTCTGGTCCCat                      >  1:262655/1‑140 (MQ=255)                                                                                                                      tAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTCCCATAtt                   >  1:404421/1‑140 (MQ=255)                                                                                                                      tAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTCCCATAtt                   >  1:470283/1‑140 (MQ=255)                                                                                                                      tAACAGGGGCGGCATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTCCCATAtt                   >  2:586782/1‑140 (MQ=255)                                                                                                                                ggcATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTCAAGGCCGCCAGGAACTCTGGTGACATATTCAGCTTACGc         >  2:316949/1‑140 (MQ=255)                                                                                                                                ggcATCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTCAAGACTGACAGGAACTATGGTACCATATTCAGCTTACGc         >  2:281854/1‑140 (MQ=255)                                                                                                                                   aTCGCGCTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTCCCATATTCAGCTTACGCAgg      <  1:442099/140‑1 (MQ=255)                                                                                                                                      gcgcTCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGCCAGGAACTCT‑GACCAATATTCGGCTTACGCAggcggc  >  1:273345/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TCACTTCAGGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAATCATGAAACATCTGCGCTTACTCCTGTATTACGCACTAACAGGGGCGGCATCGCGCCCCAGATTTAATGAATAAAGATTACGCCAGTTGACGAAGCATCCGACGCAGCGGCTCCGCGGCCCCCCACAGCAGCTGGTCGCCCACGGTAAAGGCTGACAGGAACTCTGGTCCCATATTCAGCTTACGCAGGCGGC  >  NC_000913/3573617‑3573890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑