| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,576,313 | A→G | V250A (GTG→GCG) | gntU ← | low‑affinity gluconate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,576,313 | 0 | A | G | 96.0% | 167.4 / NA | 50 | V250A (GTG→GCG) | gntU | low‑affinity gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (28/20); minor base C (0/2); total (28/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCACAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAA > NC_000913/3576186‑3576448 | cACTTTGTCTTTTGGCATGCCCCGACGCATTTCCATACCTTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCgg < 2:324189/140‑1 (MQ=255) tCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGt > 2:77704/1‑140 (MQ=255) tttGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGtttt < 2:647573/140‑1 (MQ=255) tGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCa > 1:61705/1‑140 (MQ=255) ggCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTGGTAAGCAGGTGAACCTGCCGGCGCAGAACGGGCGGCAATGGTTTTCAg > 2:207535/1‑140 (MQ=255) cATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGcc > 1:35958/1‑140 (MQ=255) tGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCgg > 2:710574/1‑123 (MQ=255) tGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCgg < 1:710574/123‑1 (MQ=255) tGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCt > 2:142928/1‑140 (MQ=255) cccTGACGCATTGCCGGACCGTGAAGCGCGACCAGACGAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTac < 1:190290/140‑1 (MQ=255) ccTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCGGTTGATCCTTCCGGCGTAAAACGCGGGGCACTGGTTTTCAGCCCTacc > 2:210500/1‑140 (MQ=255) aTTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTaccagcaccag < 1:605411/140‑1 (MQ=255) tGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCg < 1:632564/140‑1 (MQ=255) tGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCg > 2:632564/1‑140 (MQ=255) tGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTCCCGGCGCACAACGCGCGGCAATGGTCTTCACCCCTACCAGCACCAGCg > 2:190290/1‑140 (MQ=255) ccAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACACGCGGCAGTGGGTTACAGCCCGACCAGCACCAGCGgc > 1:214169/1‑140 (MQ=255) aCCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGgcagca > 2:70595/1‑140 (MQ=255) tAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGAt > 2:60756/1‑140 (MQ=255) tAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGAt > 1:462254/1‑140 (MQ=255) gCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGa < 2:675054/140‑1 (MQ=255) caccaGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACa > 1:629415/1‑140 (MQ=255) accaGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAg > 2:153665/1‑140 (MQ=255) ccaGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAgg > 2:710646/1‑140 (MQ=255) gACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGACCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTg > 2:241291/1‑140 (MQ=255) aCAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGa > 1:53567/1‑140 (MQ=255) gCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCt < 1:436225/134‑1 (MQ=255) gCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCt > 2:436225/1‑134 (MQ=255) gCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATcc > 2:508/1‑140 (MQ=255) aCCAGAATCGCGGTAAACGGATGATCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGaa > 2:485726/1‑140 (MQ=255) aaaCGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGc < 2:47712/140‑1 (MQ=255) aaCGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGcc < 1:469696/140‑1 (MQ=255) aCCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCCGGATCAGCGACATGCTGAATCCGAAGGATGGCATTTTGCCTTCGCCga > 2:300864/1‑140 (MQ=255) aTAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATg < 1:710646/140‑1 (MQ=255) aaaCTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCGGCGACAGGGTGATTCCAAAAGATGGAATTTAGCCTTCGCCGAGATGCg > 2:192961/1‑140 (MQ=255) cTCAAACCATTCGTATGCAGTTTATTCTTTCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGtt < 1:273895/140‑1 (MQ=255) cTCAAACCATTCGTACGCAGTTCATGCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGtt < 1:339652/140‑1 (MQ=255) cATTCGTAAGCAGTTGATCCTTGCGGCGCAACACGGGCGCCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGAt < 2:214169/140‑1 (MQ=255) gTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt < 1:142928/140‑1 (MQ=255) gTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt < 1:612045/140‑1 (MQ=255) gTAAGCAGTTGATCCTTCCGGCCCAAAACCCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt < 1:300864/140‑1 (MQ=255) aGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt > 1:692261/1‑137 (MQ=255) aGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt < 2:692261/137‑1 (MQ=255) cAGTTGATCCTTCCGGCCCAAACCGCGCGGCTATGCTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGa < 2:214542/140‑1 (MQ=255) ttGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCATTCGCCGGGATGCGCTTCGCTGATGTCGTCAGGAata > 1:214542/1‑140 (MQ=255) gATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATg < 1:438575/140‑1 (MQ=255) ccTTCCGGCGCAAAACGCGCGGCAATGGTCTTCAGCCGTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAg < 1:210500/140‑1 (MQ=255) ttCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCt > 1:442297/1‑140 (MQ=255) cGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGGGATCCGGTTCGCTGATATCGTCAGGAATATGCAGCTCaa > 2:297946/1‑140 (MQ=255) cGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt > 2:636209/1‑122 (MQ=255) cGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt < 1:636209/122‑1 (MQ=255) | CACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCACAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAA > NC_000913/3576186‑3576448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |