Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,606,118 C→T T123M (ACG→ATG)  yhhN → conserved inner membrane enzyme YhhN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,606,1180CT100.0% 153.3 / NA 47T123M (ACG→ATG) yhhNconserved inner membrane enzyme YhhN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/20);  total (27/20)

GCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGACGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCGTTG  >  NC_000913/3605992‑3606251
                                                                                                                              |                                                                                                                                     
gcgcGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAagag                                                                                                                          <  2:731597/140‑1 (MQ=255)
gcgcGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAagag                                                                                                                          <  1:429065/140‑1 (MQ=255)
     tttttCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGATACTGGCAATTATCAGGATGCTCCTGGAAGAGTACCg                                                                                                                     >  1:219213/1‑140 (MQ=255)
                            cacCATCTATTTCGCCAGTCAGATGACGCTCTCCTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCg                                                                                              >  2:719753/1‑140 (MQ=255)
                               cATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCa                                                                                           <  2:664094/140‑1 (MQ=255)
                               cATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCa                                                                                           <  1:727853/140‑1 (MQ=255)
                                 tCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATg                                                                                         >  2:441577/1‑140 (MQ=255)
                                 tCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATg                                                                                         >  2:107829/1‑140 (MQ=255)
                                 tCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATg                                                                                         >  1:463499/1‑140 (MQ=255)
                                 tCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATg                                                                                         >  2:619315/1‑140 (MQ=255)
                                 tCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATg                                                                                         <  2:721218/140‑1 (MQ=255)
                                 tCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGAATGCGCCTAGAATAGTACCGTTGGCCTATCGGCACGTTTATCGGCATg                                                                                         >  2:202039/1‑140 (MQ=255)
                                 tCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGAATGCGCCTAGAAAAGTCCCGTTGGCATATCTGCACGTTTATCGGCATg                                                                                         >  2:272810/1‑140 (MQ=255)
                                        cGCCAGTCAGATGACCCTCTCTCTCTTCTCGCCTCTAACACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTgg                                                                                  <  1:202039/140‑1 (MQ=255)
                                             gTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATg                                                                             >  2:120523/1‑140 (MQ=255)
                                                   tGACGCTCTCTTTCTTCCGGCCGCTACCACTCGTGTTGCAGGTTCTGGATGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGtgg                                                                       <  2:219213/140‑1 (MQ=255)
                                                     aCGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGtggct                                                                     <  1:615106/140‑1 (MQ=255)
                                                       gCTCTCCTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGtggctgg                                                                   <  1:719753/140‑1 (MQ=255)
                                                        ctctctTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGtggctggc                                                                  >  2:409751/1‑140 (MQ=255)
                                                                     ggCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAAc                                                          <  2:6546/135‑1 (MQ=255)
                                                                     ggCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAAc                                                          >  1:6546/1‑135 (MQ=255)
                                                                     ggCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTgg                                                     >  1:658593/1‑140 (MQ=255)
                                                                     ggCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTgg                                                     >  1:727255/1‑140 (MQ=255)
                                                                     ggCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTAGCAGGTGAACTGTgg                                                     >  2:165376/1‑140 (MQ=255)
                                                                     ggCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGCTGAGGGGGTGGCTAGCAGGTGAACTGTgg                                                     >  1:239332/1‑140 (MQ=255)
                                                                           tACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGttcttc                                               >  1:577660/1‑140 (MQ=255)
                                                                           tACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGttcttc                                               >  2:542836/1‑140 (MQ=255)
                                                                           tACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCGGGTGATGGTGTGGCGGGCAGGAGAACTGTGGttcttc                                               >  1:217799/1‑140 (MQ=255)
                                                                                cTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTtccgtcc                                          >  1:512575/1‑140 (MQ=255)
                                                                                   gTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGAc                                       <  1:409751/140‑1 (MQ=255)
                                                                                   gTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGAc                                       >  2:538339/1‑140 (MQ=255)
                                                                                     gctgctGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCg                                     >  1:646440/1‑140 (MQ=255)
                                                                                      ctgctgGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGc                                    >  2:173265/1‑140 (MQ=255)
                                                                                       tgctgGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCt                                   >  1:421560/1‑140 (MQ=255)
                                                                                            gTTCTGGGTGCGCTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGgc                              >  1:697980/1‑140 (MQ=255)
                                                                                             ttCTGGGTGCGCTGTTATTGGCAATTATCTGAATGCGCCTGGAAGAGTCCCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGgcg                             <  2:217799/140‑1 (MQ=255)
                                                                                             ttCTGGGTGCGCTGTCACTGGCCATCATCCGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGgcg                             <  1:202352/140‑1 (MQ=255)
                                                                                                      cgcTGTTACTGGCGATTATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGcc                    >  1:736040/1‑139 (MQ=255)
                                                                                                                 gCGATTATCTGGATGAGCCTGGAAGAGTATCGTTGGCATATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGgcgc         <  1:272810/140‑1 (MQ=255)
                                                                                                                     ttATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCg                    <  1:441270/125‑1 (MQ=255)
                                                                                                                     ttATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCg     >  1:430451/1‑140 (MQ=255)
                                                                                                                     ttATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGGCCGACCGCTCCGGCGCTCTCTGCg                    >  2:441270/1‑125 (MQ=255)
                                                                                                                      tATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCGt    <  1:173265/140‑1 (MQ=255)
                                                                                                                      tATCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCGt    <  2:421560/140‑1 (MQ=255)
                                                                                                                        tCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCGTTg  <  2:374734/140‑1 (MQ=255)
                                                                                                                        tCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCGTTg  <  1:120523/140‑1 (MQ=255)
                                                                                                                        tCTGGATGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCGTTg  <  2:736040/140‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
GCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGACGCGCCTGGAAGAGTACCGTTGGCCTATCTGCACGTTTATCGGCATGACGCTGGTGATGGTGTGGCTGGCAGGTGAACTGTGGTTCTTCCGTCCGACCGCTCCGGCGCTCTCTGCGTTTGTTGGCGCTTCGTTG  >  NC_000913/3605992‑3606251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑