Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,501	G→A	A13V (GCG→GTG)	rbbA ←	ribosome‑associated ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,501	0	G	A	100.0%	112.3 / NA	33	A13V (GCG→GTG)	rbbA	ribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/18);  total (15/18)

AACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGAT  >  NC_000913/3629369‑3629635                                                                                                                                     |                                                                                                                                       aaCGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAgg                                                                                                                                 <  1:42236/140‑1 (MQ=255)        acaaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGcgggacg                                                                                                                          <  1:97030/140‑1 (MQ=255)             cTCGACTTCCCGACGTCGTCCGTGCCAATCAGCCCGACCATACAGCGGGCCGAAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAAc                                                                                                                     <  1:198977/140‑1 (MQ=255)                               tCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                                                                   >  2:160567/1‑140 (MQ=255)                               tCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                                                                   >  2:137220/1‑140 (MQ=255)                                        aTCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAc                                                                                          <  2:633852/140‑1 (MQ=255)                                        aTCAGCCCGACCATACAGCGGGACGGAATATCGAGTGTGTTATTGTTCTGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAc                                                                                          <  2:232133/140‑1 (MQ=255)                                         tCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTGCAGATGGGTCATTGCGGCAAcc                                                                                         >  1:232133/1‑140 (MQ=255)                                         tCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                                                         >  2:672078/1‑140 (MQ=255)                                              ccGACCATACAGCGGGCGGGAATATCGAGAGTGATCTTGTTCAGCGCAGCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacc                                                                                    <  2:272027/140‑1 (MQ=255)                                                 aCCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                                                                 <  2:572566/140‑1 (MQ=255)                                                 aCCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                                                                 <  1:503320/140‑1 (MQ=255)                                                            ggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAgg                                                                      >  1:686932/1‑140 (MQ=255)                                                            ggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGGCGTCAgg                                                                      >  2:675566/1‑140 (MQ=255)                                                               cGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCa                                                                   <  1:680779/140‑1 (MQ=255)                                                                  aaTATCGAGAGTGATATTGTTCTGCCCAACGGTTTTTTCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACgg                                                                <  1:324651/140‑1 (MQ=255)                                                                               atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGtt                                                           <  1:555597/132‑1 (MQ=255)                                                                               atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGtt                                                           >  2:555597/1‑132 (MQ=255)                                                                               atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattc                                                   >  2:139115/1‑140 (MQ=255)                                                                               atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAGTGCGGCAAGCTCACCACGAGGTCGTGAGGCGACGGAAGTTCttcattc                                                   >  2:215484/1‑140 (MQ=255)                                                                                  ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAcc                                                <  2:686932/140‑1 (MQ=255)                                                                                   tGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC‑AGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg                                              <  1:160567/140‑1 (MQ=255)                                                                                    gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg                                              <  1:394764/140‑1 (MQ=255)                                                                                       cAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAc                                           >  1:21134/1‑140 (MQ=255)                                                                                          cgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCa                                        >  1:636293/1‑140 (MQ=255)                                                                                                       ccATAATGCTGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGc                           >  2:607876/1‑140 (MQ=255)                                                                                                                tGGCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGtt                  <  2:636293/140‑1 (MQ=255)                                                                                                                  gCTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGtttt                >  1:124931/1‑140 (MQ=255)                                                                                                                   cTCACGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTg               >  1:442564/1‑140 (MQ=255)                                                                                                                      aCGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACa            <  2:442564/140‑1 (MQ=255)                                                                                                                      aCGCCCGCCAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACa            >  2:456930/1‑140 (MQ=255)                                                                                                                       cgcccgccAGTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACat           <  1:672078/140‑1 (MQ=255)                                                                                                                                gTTGCACGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGAt  <  1:160693/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       AACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGAT  >  NC_000913/3629369‑3629635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑