Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	505,107	G→A	G65D (GGT→GAT)	ushA →	5'‑nucleotidase/UDP‑sugar hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	505,107	0	G	A	100.0%	175.5 / NA	51	G65D (GGT→GAT)	ushA	5'‑nucleotidase/UDP‑sugar hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/24);  total (27/24)

AACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGGTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGG  >  NC_000913/504976‑505232                                                                                                                                    |                                                                                                                              aaCTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCa                                                                                                                       <  2:393065/140‑1 (MQ=255)   cTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCaaa                                                                                                                     >  1:52836/1‑140 (MQ=255)         tGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCACAAAAAACGCTGGTGGATGATATCCGCAAAGAGGtt                                                                                                               >  1:614117/1‑140 (MQ=255)           gCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACCCTGGTGAATGATACCCGCAAAGAGGTTGc                                                                                                             >  1:325900/1‑140 (MQ=255)                tGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTg                                                                                                        >  1:511830/1‑140 (MQ=255)                tGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGAAGATATCCGCAAAGAGGTTGCGGCTg                                                                                                        >  1:349961/1‑140 (MQ=255)                 gAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGa                                                                                                       <  1:41411/140‑1 (MQ=255)                     aGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGc                                                                                                   >  1:14750/1‑140 (MQ=255)                        aTAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCACAAAGAGGTCGCGGCTGAAGGCGGt                                                                                                >  1:305280/1‑140 (MQ=255)                         tAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTa                                                                                               <  2:542054/140‑1 (MQ=255)                              ccTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtg                                                                                          >  1:560928/1‑140 (MQ=255)                              ccTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtg                                                                                          <  2:732250/140‑1 (MQ=255)                               cTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgc                                                                                         <  2:15346/140‑1 (MQ=255)                               cTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgc                                                                                         <  2:511830/140‑1 (MQ=255)                                 aCAAAATTTCAGTTCTTCATACCAATGATCGTCGTGGGCATTTTTGGCTCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                       <  2:349961/140‑1 (MQ=255)                                 aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                       >  1:132085/1‑140 (MQ=255)                                 aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                       >  2:526242/1‑140 (MQ=255)                                 aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                       >  2:702920/1‑140 (MQ=255)                                 aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                       >  1:48821/1‑140 (MQ=255)                                 aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                       <  2:614117/140‑1 (MQ=255)                                 aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATAGCCGCAAAGAGGATGAGGCTGAAGGCGGTAGCGtgctg                                                                                       >  1:192470/1‑140 (MQ=255)                                 aCAAAATTACAGTTCTGAATACTAATGATCATCATAGGCCTTTTTGGCTCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                       <  2:192470/140‑1 (MQ=255)                                                tGCATACCAATGATCCTCATGGCCACTTTCGGCGCAACGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGc                                                                        <  1:221458/140‑1 (MQ=255)                                                tGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGc                                                                        <  2:373972/140‑1 (MQ=255)                                                tGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGc                                                                        <  2:14750/140‑1 (MQ=255)                                                tGCACACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGc                                                                        <  2:365763/140‑1 (MQ=255)                                                 gCATACCAATGATCACCATGGGCATATTTCGCGCAATCAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCg                                                                       <  1:209930/140‑1 (MQ=255)                                                    tACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACa                                                                    >  1:147863/1‑140 (MQ=255)                                                     aCCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACAt                                                                   <  2:132085/140‑1 (MQ=255)                                                     aCCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACAt                                                                   <  1:180944/140‑1 (MQ=255)                                                       cAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTa                                                                 >  2:52514/1‑140 (MQ=255)                                                               atcatGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGc                                                         >  1:415139/1‑140 (MQ=255)                                                               atcatGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCCGAAGGCGGAAGCGTGCAGCTACTTTACGGTGGCGACATTAACACTGGc                                                         >  2:232379/1‑140 (MQ=255)                                                                 catGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGt                                                       >  1:180629/1‑140 (MQ=255)                                                                     ggCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGccc                                                   <  2:52836/140‑1 (MQ=255)                                                                      gCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGC‑‑TAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAg                                                >  2:64743/1‑140 (MQ=255)                                                                       cATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGa                                                 >  2:634599/1‑140 (MQ=255)                                                                          ttttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTc                                              >  2:659926/1‑140 (MQ=255)                                                                          ttttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTc                                              <  1:526242/140‑1 (MQ=255)                                                                                 gcAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTa                                       <  2:492555/140‑1 (MQ=255)                                                                                 gcAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTa                                       <  2:180629/140‑1 (MQ=255)                                                                                         tatGGCGAATATGGTGTGGCGGAGCGAAAAACGCTGGGGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGc                               <  1:297950/140‑1 (MQ=255)                                                                                         tatGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGc                               <  2:48821/140‑1 (MQ=255)                                                                                                 atatGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTg                       >  1:434770/1‑140 (MQ=255)                                                                                                 atatGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTg                       >  1:482662/1‑140 (MQ=255)                                                                                                 atatGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTg                       >  1:618731/1‑140 (MQ=255)                                                                                                 atatGGTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCAACATTAACCCTGGCGTTCCCGAGTCTGACTCACAGGATGCCGAACCTg                       >  2:309138/1‑140 (MQ=255)                                                                                                      gTCTGGCGGCGCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGAtttt                  <  1:659926/140‑1 (MQ=255)                                                                                                         tGGCGGCGCAAAAAACACTGGTTGATGATATTCGCTAAGAGGTTGCGGGTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcgc               <  2:325900/140‑1 (MQ=255)                                                                                                            cggcgCAAAAAACGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGt            >  2:91404/1‑140 (MQ=255)                                                                                                                      aaCGCTGGTGGATGATATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCtgg  >  2:649576/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                              AACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGGTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGG  >  NC_000913/504976‑505232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑